LOCUS       sequence-42705-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Muona M, Aranko AS, Raulinaitis V, Iwai H
  TITLE     Segmental isotopic labeling of multi-domain and fusion proteins by 
            protein trans-splicing in vivo and in vitro.
  JOURNAL   Nat Protoc. 2010  . 5(3):574-87.
  PUBMED    20203672
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Protoc.
            2010  . 5(3):574-87."
COMMENT     Sequence Label: pHYRSF1
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..65
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             75..92
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             604..648
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(686..704)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      700..747
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aataattttg tttaacttta ataaggagat ataccatggg cagcagccat catcatcatc
       61 atcacagcag cggcctggtg ccgcgcggca gccatatgca gtacaaactt atcctgaacg
      121 gtaaaaccct gaaaggtgaa accaccaccg aagctgttga cgctgctacc gcggaaaaag
      181 ttttcaaaca gtacgctaac gacaacggtg ttgacggtga atggacctac gacgacgcta
      241 ccaaaaccta caccgtaacg gaaggatcct gtttaagcta tgaaacggaa atattgacag
      301 tagaatatgg attattaccg attggtaaaa ttgtagaaaa gcgcatcgaa tgtactgttt
      361 atagcgttga taataatgga aatatttata cacaacctgt agcacaatgg cacgatcgcg
      421 gagaacaaga ggtgtttgag tattgtttgg aagatggttc attgattcgg gcaacaaaag
      481 accataagtt tatgactgtt gatggtcaaa tgttgccaat tgatgaaata tttgaacgtg
      541 aattggattt gatgcgggtt gataatttgc cgaattaagc ttgcggccgc actcgagtct
      601 ggtaaagaaa ccgctgctgc gaaatttgaa cgccagcaca tggactcgtc tactagcgca
      661 gcttaattaa cctaggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc
      721 tctaaacggg tcttgagggg ttttttgctg aaacc
//
