LOCUS       sequence-42713-e         875 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 875)
  AUTHORS   Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N
  TITLE     Abr and Bcr, two homologous Rac GTPase-activating proteins, control 
            multiple cellular functions of murine macrophages.
  JOURNAL   Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.
  PUBMED    17116687
REFERENCE   2  (bases 1 to 875)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2007-02"; volume: "27(3)"; pages: "899-911. Epub 2006
            Nov 20"
COMMENT     Sequence Label: pCDNA3.1Xpress-AbrR683AN795A-HisC
FEATURES             Location/Qualifiers
     source          1..875
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             119..136
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             140..172
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(176..184,185..199)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagctctctg gctanctaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagcgtt taaacttaag cttaccatgg ggggttctca
      121 tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg gtcgggatct
      181 gtacgacgat gacgataagg taccaggatc catggaggag gaagaggagg cgataggctt
      241 gctggacaag gttctggagg acgaagatgt gtttctcctg ggggagtgcg agctgggaac
      301 cccgaccagc cccggctcag ggtccccctt cctggtggct gtgaaggtgg aagcagggaa
      361 aggcctggag atgaggaagc tggttctctc ggggttcttg gccagcgaag agatctacat
      421 taaccagctg gaagccctgt tgctgcccat gaaacccctg aaggccaccg ccaccacctc
      481 ccagcccgtg ctcaccatcc agcagatcga gaccatcttc tacaagatcc aggacatcta
      541 tgagatccac aaggagttct atgacaacct gtgccccaag gtgcaacagt gggacagcca
      601 ggtcaccatg ggccacctct tccagaagct ggccagccag ctcggtgtgt acaaagcgtt
      661 tgtcgataac tataaagtcg ctctggagac agctgagaag tgcagccagt ccaacaacca
      721 gttccagaag atctcagagg aactcaaagt gaaaggtccc aaggactcca aggacagcca
      781 cacgtctgtc accatggaag ctctgctcta caagcccatt gaccgggtca ctcggagcac
      841 cctagtccta cacgacctgc tgaagcacac acctg
//
