LOCUS       sequence-427148-        9033 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of V5 tagged USP47.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9033)
  AUTHORS   Kassel S, Hanson AJ, Benchabane H, Saito-Diaz K, Cabel CR, Goldsmith
            L, Taha M, Kanuganti A, Ng VH, Xu G, Ye F, Picker J, Port F, Boutros
            M, Weiss VL, Robbins DJ, Thorne CA, Ahmed Y, Lee E
  TITLE     USP47 deubiquitylates Groucho/TLE to promote Wnt-beta-catenin 
            signaling.
  JOURNAL   Sci Signal. 2023 Feb 7;16(771):eabn8372. doi: 
            10.1126/scisignal.abn8372. Epub 2023 Feb 7.
  PUBMED    36749823
REFERENCE   2  (bases 1 to 9033)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-02-7"; volume: "16(771):eabn8372. doi"; pages:
            " 10.1126/scisignal.abn8372. Epub 2023 Feb 7"
COMMENT     Sequence Label: Human V5-USP47
FEATURES             Location/Qualifiers
     source          1..9033
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(57..98)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          159..634
                     /label=beta-globin intron
                     /note="internally truncated intron from human beta-globin"
     promoter        complement(768..970)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(797..817)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        972..1275
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     polyA_signal    1356..1404
                     /note="synthetic polyadenylation signal"
     terminator      1422..1468
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     misc_feature    1473..1544
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(1696..1832)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     CDS             complement(join(1845..2636,2637..2705))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     2468..2487
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3451..4039
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3940..3959
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     misc_feature    4265..4336
                     /label=3' beta-globin insulator
                     /note="insulator downstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(4381..4517)
                     /label=rrnG terminator
                     /note="transcription terminator from the E. coli ribosomal 
                     RNA rrnG operon (Albrechtsen et al., 1991)"
     terminator      complement(4547..4593)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    4708..4829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4745..4764)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4799..4818
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tcctttggaa ccagttggtt ttcttcccct ggcaccatgg agccgctgcc actgcctgtg
       61 ctatcgaggc cgagtaaagg attggggatg ggttttccca tgcggccgcc tttgccaaag
      121 tgatgggcca gcacacagac cagcacgttg cccaggagct gtgggaggaa gataagaggt
      181 atgaacatga ttagcaaaag ggcctagctt ggactcagaa taatccagcc ttatcccaac
      241 cataaaataa aagcagaatg gtagctggat tgtagctgct attagcaata tgaaacctct
      301 tacatcagtt acaatttata tgcagaaata tttatatgca gaaatattgc tattgcctta
      361 acccagaaat tatcactgtt attctttaga atggtgcaaa gaggcatgat acattgtatc
      421 attattgccc tgaaagaaag agattaggga aagtattaga aataagataa acaaaaaagt
      481 atattaaaag aagaaagcat tttttaaaat tacaaatgca aaattaccct gatttggtca
      541 atatgtgtac cctgttactt ctccccttcc tatgacatga acttaaccat agaaaagaag
      601 gggaaagaaa acatcaaggg tcccatagac tcaccctgaa gttctcagga tccgagctcg
      661 gtaccacatg taagcttcga ggggaggctg gatcggtccc ggtgtcttct atggaggtca
      721 aaacagcgtg gatggcgtct ccaggcgatc tgacggttca ctaaaccagc tctgcttata
      781 tagacctccc accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga
      841 cattttggaa agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg
      901 tggagacttg gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa
      961 aaccgcatca gcatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag
     1021 tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca
     1081 atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt
     1141 aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac
     1201 gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc
     1261 gtaagttatg taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat
     1321 tactagatct ggctacactc gatcagcagt tagataataa aatcgctatc catcgaagat
     1381 ggatgtgtgt tggttttttg tgtgtgtaac gcaacgattg atagcataac cccttggggc
     1441 ctctaaacgg gtcttgaggg gttttttgaa ttgagaggta gctgaagctg ctgttatgac
     1501 cactagaggg aagaagatac ctgtggagct aatggtccaa gatgtcggga gccgcgatcg
     1561 ctgccaaaaa ggtaagctga cgatagctga cgagattcga tgcccgacga gcaaatgagc
     1621 gccacggctg ttcgcgtaag ctcgaaacga cggagattcg atagagccag agtcgagttt
     1681 aaacgattcg ggatcttaag gataaatttc tggtaaggag gacacgtatg gaagtgggca
     1741 agttggggaa gccgtatccg ttgctgaatc tggcatatgt gggagtataa gacgcgcagc
     1801 gtcgcatcag gcattttttt ctgcgccaat gcacctagaa tcggttacca atgcttaatc
     1861 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     1921 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     1981 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     2041 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat caactgttgc
     2101 cgggaagcta gagtaagtag ttcgccagtt aatagtttac gcaaggttgt tgccattgct
     2161 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     2221 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     2281 cctccaatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     2341 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgaatac
     2401 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     2461 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaagcgt
     2521 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     2581 actcgcgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     2641 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     2701 ctcatgcgaa agcctcctgc tgatcctgtc tcttgataga tgtttgcaaa agctaggact
     2761 cgattatacg ttgaccgatc agactatcaa tatctaggtt taaacagatc ggtacgctgc
     2821 cgtgaccagg cttgagtaca atatctgacg attggacttc ctgggtccca agctggattc
     2881 cgactggctg actgccaagc aacgatatgg atcgtcgaga ctcgcactga gaggatcgtt
     2941 ctcggtacac caggcgcgcc ccgatttcga tattcctcgg atctcggata gatcgggatt
     3001 gaggatgtag gttgaacttc tctcgggtga tcccgatctc ggatattcgg gattggagta
     3061 atacctggcg ataggcgctg ccaagcaata gccaaggcgc aaggccggcc aagagacttg
     3121 cgatagttcg ttcgagaggt agcctgcaag atggatcgga cgaaaggttt gaagattttc
     3181 cgacgagggc cttggagagt tcggaaattt aaatgcaact atggatgaac gaaatagaca
     3241 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3301 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3361 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3421 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3481 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3541 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3601 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3661 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3721 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3781 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3841 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     3901 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     3961 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4021 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4081 ctggcctttt gctcacatga tttaaatgaa actcgtaatc ggagaacggg tgcaatagca
     4141 aggaccagat cgaacttgac tgacgcaatt ctgagcgtag tcgaaaccca gacaagtcga
     4201 tctcgccaag tagctgactt gactggttaa ttaagtttcg atagcccaag gtaaccaatg
     4261 agattaggga gggaagaaag tttgatgaac tacttctgac ccctagtggt gtccagaaaa
     4321 gaccattaaa ggaatgtctt gatcgaaatc gagagatcgc ctgcaggata gctgacgacc
     4381 ttaaggataa atttctggta aggaggacac gtatggaagt gggcaagttg gggaagccgt
     4441 atccgttgct gaatctggca tatgtgggag tataagacgc gcagcgtcgc atcaggcatt
     4501 tttttctgcg ccaatgccgg tagatagcaa tccggcccga ggggcacaaa aaacccctca
     4561 agacccgttt agaggcccca aggggttatg ctacgatacc gatagagatg ggccaatacc
     4621 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     4681 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4741 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4801 ggtttgtcca aactcatcaa tgtatcttat catgtctgtg agctgaaggt acgctgtatc
     4861 tcagtcagtc aagctagcag aggcctgcgg atccagaaat cgattgtatc agtcagtcag
     4921 tgcaggagga gacaacttct agatcagtcc tgcgtcaaat ccttgttggg agcaccatcc
     4981 agatagattt tcaaggcttt ctctttcctt ggactatatg tcacgcgatg accggtcttt
     5041 tgcagtctgg agctctcctt cttcatgagt tcgttgcgtt gctcgtcggt caactccata
     5101 agttcttctg ttttatcgcg ataaaagatg accgctccat catcgcatat atacagaggc
     5161 cacacgttaa gtgtactcac tttagggttc caatccaagt cttggtggat atcaaggaca
     5221 gagatgtcgc aagggaaggt acctctgccc tttgcaaatt cgatatcatc aagcggtatc
     5281 ccgcttattt cgctcaactt ctctcgcagc tcatccacgg agctagattc cagcacgact
     5341 tcctggaatg ggtctaactt catctcactc ggcttccatc gccttgaaag taccgcgagc
     5401 tgactcatag atttcatctt ttccacacca tccaagactt ctaagaatac ttcccagttg
     5461 cttgaaatgt taatgtcttc ttcgtatatg tggtaatcaa ggaagacggt gccgggattc
     5521 ttccaagtct tcttcctcag acgaaatcga tcaatggata actcaagtcc gcattgctct
     5581 ctgagttgcg ggatcagctc ttccttgctc tgtctgacgg tcatcccctt agcgaagacg
     5641 gcgtcaagga gaaatttaca gggttcctgt tcgttcacca gcaactgata aactttcaca
     5701 cgatattctc ccttcttcag tgctctgccc agccgtatag tgatcttgtt gtcgtctgag
     5761 aatgagctca gagtttcgtt taagcggaca gattcgaact cttggttaga ggcgtaaact
     5821 cggaacactt tgaaatgaga actaaggacc ccgacgaatg gttctaagtg ttgcttaaag
     5881 gctgcaaggg taatccgttt atccacgtgg accatcaacc acttgtgacc ctcacctgac
     5941 ccttcgtccg cggcgtaagg ctcagctttg aaatacatat attgcatctc tcccctggac
     6001 ttaccggatt catcatattc gctatcggtt ccggaatctt cctctgcggt gtcccaagtc
     6061 tctttcttcc cttcattcgc tttagtgcgt ctgctgctgg tgattgagtg ggaatccaat
     6121 ccgttcgcca aggggatctg tgcattgtca gcattacaga gcgtgtcgct actatgagag
     6181 gatgagagaa tatcggagtc cacagagctg gtagaacggt cgttgtttac gtcactatca
     6241 gatctctctt cactttgaaa attctcaggg tcagacgtct gtatatgttg ttccaactct
     6301 ctattgtcaa cagatgcaga ggaatccctt tcgtttaagg gtgattcgat attctcgaag
     6361 tcgctagttt cggttgattt agaagagtct ccgttatcgc cgtcctgttg ttgttgcaag
     6421 cttaaggatt tcaacttttc cgtgctttct tctaaaatag cctcgacact tttcaaggat
     6481 cccacggggc ctttgacccg ctcacagtcg tcatctacat tcccagaatc gccgccggcc
     6541 ttttgatatg ctgtcctttt agagtaactg actggggact gttcaggcaa gagaacgaaa
     6601 agtctgattg tgttagcatg cctatccaaa agtttccaga gatgggagtc tgcgaaggcc
     6661 atttggtagt cgagcgtttc ggagctctcg acgaaaacct tattggacct gaagaagcct
     6721 tcagctttga gagtcttact tgagacggac aagagacgga gatcattgta acaacgttcg
     6781 aggactatcc tcatcgtctc tgcagggaga tggattgctt tacttatcag ttgcttgaat
     6841 tcagtcaccg tttgattcaa gtatgccctc acagtaattg gagcagcgac ggattcggcc
     6901 ttcaggtcaa ctacatgtac tttaaccatg acctcgcccg gtttatatga ttgaaatact
     6961 tggtcgggct tccgggtctc aaggagcaaa tcaaacatat aagttgactt gacgccgccc
     7021 agcagcagcc ccatgggggt atcttcttcg ccctcataag agcgctccag ataatcatga
     7081 aactcgtcat atttcacaag gcgacaacag tccaatggaa taacttcctc gagatccatc
     7141 atcttgtaag ccatctcaac tgcctctttt aaagttttgt ctttatgcac ttccaacttg
     7201 ttttccatca tcacttgttt ggtgggatgg agacaaaaca gcttgatttt acaggtgttt
     7261 ctttcgattt ctcgttgtcg cttttcctgc tcctcgagct ctcgttcttt ctgcactaaa
     7321 ttcttaatat gctctgggta ttcatccact tccagaaact tagcgttccg ggccggatct
     7381 ttcaaccgat atatgagcat ataggcattt gtgcttgagg cgaaagcgga gctatagtac
     7441 ccacgacttc ctgaggatcc gccatgcgtt ttctttatat cttcttgggt gatgcgggat
     7501 acatgttgat cgttgaaact ataccattgc tcatcggaga atgactttat acaggcgtag
     7561 tagtgcccgc cagcggcgga tccggaatga accatgacgg agaataactc gtatatgagg
     7621 gagttcttct caagtccact tttagagatc ttttcagtcc cagagtttgt ttccaggcag
     7681 attccctcgt cgactccatc gtcattggag aagtcattag acatttggtc agaatggcag
     7741 ctgccttcgt tttccgctcc tgaatctgta cagctctcag tctgaggact tttctcatct
     7801 tctacatcaa tgaacgtact catgtcgagt tcctctggaa atgtcatgcg atcgtttaat
     7861 ttaatacgat gcatggtggt ataatcaaaa tcgaagcgtt tgagctggag tgtcaaaagg
     7921 tatggaaagt gaagaaaacg caagccttta cgcgcgtcgc atttcttctt gcacctttcg
     7981 cagaaatact ggttggggcc gtccaggatc tctggttgga taaaagcatg caaagcttct
     8041 tccacggacg cgaaagcctg gctagatccg tatgggcgta ttacaagggg tatatccagg
     8101 taggtgtcta tgcgccaacc ttcatatccg cattccagac aacgtacgta gtcttttaac
     8161 ttgccttgat agagctcgtt gattaaatct gcttgttcag tttgtttcca cttttgctcg
     8221 agtgcgtcaa acatcaccct gcaaagctct tggacatcgt gttgttgcca agcctcagaa
     8281 gagtcccatc cgaaggacct cgtcacgtcg gtggtttcaa tagcccgctt cttagaagtc
     8341 tgcaggagaa caaacagcct ctggagttga taagggatag atgtcactgg atcttcctcg
     8401 ctctcctcaa attcccattt atagagagcg ttccggaact cgggtgtcat aaacagggtc
     8461 tgcagcaggg aatttaaata gcaagtcatc gcttgattga cgagaccgac ataaccggtt
     8521 tcagacttat ttaatatgct agaataggag tagctttgtg acacatagtc gcttgtagac
     8581 ccgccagatc cctcgcgagg aagtgggccg ataaaacgat catgtacgct atcttctcct
     8641 gcggagctat cctcgagcaa aatttgaggc tgttctccat ctttatctgt cagatgcaag
     8701 aaattcttct tcccgggttc aaaattggcg tctaataaag acttatctga cgtatgatcc
     8761 aggggagcca tgtcagcagt attaattccg ttaccccata cgagatcgaa agttccatta
     8821 atgtaaccaa ctttgttggc gacatcctca aacagtttgc gtaccggagt actagcagga
     8881 aggttgaggg tgattctctc attcacggtt ttagaattgg tggtgtcttg aataatgcac
     8941 agtacccttg gctcttctgc tgcattttct atttgcagga atttcttctt catctcatcg
     9001 aaaatatttt ggcgacaccg ccaaaacaca tct
//
