LOCUS       sequence-427151-        5844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Myc tagged XIAP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Kassel S, Hanson AJ, Benchabane H, Saito-Diaz K, Cabel CR, Goldsmith
            L, Taha M, Kanuganti A, Ng VH, Xu G, Ye F, Picker J, Port F, Boutros
            M, Weiss VL, Robbins DJ, Thorne CA, Ahmed Y, Lee E
  TITLE     USP47 deubiquitylates Groucho/TLE to promote Wnt-beta-catenin 
            signaling.
  JOURNAL   Sci Signal. 2023 Feb 7;16(771):eabn8372. doi: 
            10.1126/scisignal.abn8372. Epub 2023 Feb 7.
  PUBMED    36749823
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-02-7"; volume: "16(771):eabn8372. doi"; pages:
            " 10.1126/scisignal.abn8372. Epub 2023 Feb 7"
COMMENT     Sequence Label: Human Myc-XIAP
FEATURES             Location/Qualifiers
     source          1..5844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             31..60
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             70..99
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             133..162
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     polyA_signal    1707..1841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1731..1750)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1785..1804
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(1954..1974)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(1954..1972)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1993..2009)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1993..2009)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2006..2028)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2017..2033
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2041..2047,2048..2065,2066..2071))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2086..2107
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2224..2241)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2395..2983)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2475..2494)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3154..3945,3946..4014))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3777..3796
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4015..4119)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(4145..4600)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4282..4303)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4494..4513
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4671..5655
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        5690..5708
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     5690..5707
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             5758..5787
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             5797..5826
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(5836..5844,1..21)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
ORIGIN
        1 ctcatttctg aagaggactt gaatgaaatg gagcaaaagc tcatttctga agaggacttg
       61 aatgaaatgg agcaaaagct catttctgaa gaggacttga atgaaatgga gagcttgggc
      121 gacctcacca tggagcaaaa gctcatttct gaagaggact tgaattcagg ccggcctatg
      181 acttttaaca gttttgaagg atctaaaact tgtgtacctg cagacatcaa taaggaagaa
      241 gaatttgtag aagagtttaa tagattaaaa acttttgcta attttccaag tggtagtcct
      301 atttcagcat caacactggc acgagcaggg tttctttata ctggtgaagg agataccgtg
      361 cggtgcttta gttgtcatgc agctgtagat agatggcaat atggagactc agcagttgga
      421 agacacagga aagtatcccc aaattgcaga tttatcaacg gcttttatct tgaaaatagt
      481 gccacgcagt ctacaaattc tggtatccag aatggtcagt acaaagttga aaactatctg
      541 ggaagcagag atcattttgc cttagacagg ccatctgaga cacatgcaga ctatcttttg
      601 agaactgggc aggctgtaga tatatcagac accatatacc cgaggaaccc tgccatgtat
      661 agtgaagaag ctagattaaa gtcctttcag aactggccag actatgctca cctaacccca
      721 agagagttag caagtgctgg actctactac acaggtattg gtgaccaagt gcagtgcttt
      781 tgttgtggtg gaaaactgaa aaattgggaa ccttgtgatc gtgcctggtc agaacacagg
      841 cgacactttc ctaattgctt ctttgttttg ggccggaatc ttaatattcg aagtgaatct
      901 gatgctgtga gttctgatag gaatttccca aattcaacaa atcttccaag aaatccatcc
      961 atggcagatt atgaagcacg gacctttact tttgggacat ggatatactc agttaacaag
     1021 gagcagcttg caagagctgg attttatgct ttaggtgaag gtgataaagt aaagtgcttt
     1081 cactgtggag gagggctaac tgattggaag cccagtgaag acccttggga acaacatgct
     1141 aaatggtatc cagggtgcaa atatctgtta gaacagaagg gacaagaata tataaacaat
     1201 attcatttaa ctcattcact tgaggagtgt ctggtaagaa ctactgagaa aacaccatca
     1261 ctaactagaa gaattgatga taccatcttc caaaatccta tggtacaaga agctatacga
     1321 atggggttca gtttcaagga cattaagaaa ataatggagg aaaaaattca gatatctggg
     1381 agcaactata aatcacttga ggttctggtt gcagatctag tgaatgctca gaaagacagt
     1441 atgcaagatg agtcaagtca gacttcatta cagaaagaga ttagtactga agagcagcta
     1501 aggcgcctgc aagaggagaa gctttgcaaa atctgtatgg atagaaatat tgctatcgtt
     1561 tttgttcctt gtggacatct agtcacttgt aaacaatgtg ctgaagcagt tgacaagtgt
     1621 cccatgtgct acacagtcat tactttcaag caaaaaattt ttatgtctag gcgcgcctct
     1681 agaactatag tgagtcgtat tacgtagatc cagacatgat aagatacatt gatgagtttg
     1741 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     1801 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     1861 attttatgtt tcaggttcag ggggaggtgt gggaggtttt ttaattcgcg gccgcggcgc
     1921 caatgcattg ggcccggtac ccagcttttg ttccctttag tgagggttaa ttgcgcgctt
     1981 ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca
     2041 caacatacga gccgggagca taaagtgtaa agcctggggt gcctaatgag tgagctaact
     2101 cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct
     2161 gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc
     2221 ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     2281 ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     2341 agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     2401 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     2461 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     2521 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     2581 gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     2641 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     2701 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     2761 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     2821 cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     2881 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     2941 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     3001 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     3061 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     3121 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     3181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     3241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     3301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     3361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     3421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     3481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     3541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     3601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     3661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     3721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     3781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     3841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     3901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     3961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     4021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     4081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     4141 acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg ttaaatcagc
     4201 tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc
     4261 gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac
     4321 tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca
     4381 ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg
     4441 agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag
     4501 aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc
     4561 accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc attcaggctg
     4621 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gtcgaccgcc
     4681 aattcaatat ggcgtatatg gactcatgcc aattcaatat ggtggatctg gacctgtgcc
     4741 aattcaatat ggcgtatatg gactcgtgcc aattcaatat ggtggatctg gaccccagcc
     4801 aattcaatat ggcggacttg gcaccatgcc aattcaatat ggcggacctg gcactgtgcc
     4861 aactggggag gggtctactt ggcacggtgc caagtttgag gaggggtctt ggccctgtgc
     4921 caagtccgcc atattgaatt ggcatggtgc caataatggc ggccatattg gctatatgcc
     4981 aggatcaata tataggcaat atccaatatg gccctatgcc aatatggcta ttggccaggt
     5041 tcaatactat gtattggccc tatgccatat agtattccat atatgggttt tcctattgac
     5101 gtagatagcc cctcccaatg ggcggtccca tataccatat atggggcttc ctaataccgc
     5161 ccatagccac tcccccattg acgtcaatgg tctctatata tggtctttcc tattgacgtc
     5221 atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat tacggtaaat
     5281 ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccaccattg acgtcaatgg
     5341 gatggctcat tgcccattca tatccgttct cacgccccct attgacgtca atgacggtaa
     5401 atggcccact tggcagtaca tcaatatcta ttaatagtaa cttggcaagt acattactat
     5461 tggaagtacg ccagggtaca ttggcagtac tcccattgac gtcaatggcg gtaaatggcc
     5521 cgcgatggct gccaagtaca tccccattga cgtcaatggg gaggggcaat gacgcaaatg
     5581 ggcgttccat tgacgtaaat gggcggtagg cgtgcctaat gggaggtcta tataagcaat
     5641 gctcgtttag ggaaccgcca ttctgcctgg ggacgtcgga gcaagcttga tttaggtgac
     5701 actatagaat acaagctact tgttcttttt gcaggatccc atcgatttaa agctatggag
     5761 caaaagctca tttctgaaga ggacttgaat gaaatggagc aaaagctcat ttctgaagag
     5821 gacttgaatg aaatggagca aaag
//
