LOCUS       sequence-427152-        7770 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of USP47.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7770)
  AUTHORS   Kassel S, Hanson AJ, Benchabane H, Saito-Diaz K, Cabel CR, Goldsmith
            L, Taha M, Kanuganti A, Ng VH, Xu G, Ye F, Picker J, Port F, Boutros
            M, Weiss VL, Robbins DJ, Thorne CA, Ahmed Y, Lee E
  TITLE     USP47 deubiquitylates Groucho/TLE to promote Wnt-beta-catenin 
            signaling.
  JOURNAL   Sci Signal. 2023 Feb 7;16(771):eabn8372. doi: 
            10.1126/scisignal.abn8372. Epub 2023 Feb 7.
  PUBMED    36749823
REFERENCE   2  (bases 1 to 7770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2023-02-7"; volume: "16(771):eabn8372. doi"; pages:
            " 10.1126/scisignal.abn8372. Epub 2023 Feb 7"
COMMENT     Sequence Label: Human USP47
FEATURES             Location/Qualifiers
     source          1..7770
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        33..1010
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        1045..1063
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     1045..1062
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(1333..1350)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1333..1349)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(1351..1375)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(4808..4827)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4809..4827)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     polyA_signal    4832..4966
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4856..4875)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4910..4929
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5079..5099)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5079..5097)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5118..5134)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5118..5134)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5131..5153)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5142..5158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5166..5172,5173..5190,5191..5196))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5211..5232
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5349..5366)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5520..6108)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5600..5619)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6279..7070,7071..7139))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6902..6921
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7140..7244)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7270..7725)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7407..7428)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7619..7638
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tgcgggcctc ttcgctatta cgccagtcga ccgccaattc aatatggcgt atatggactc
       61 atgccaattc aatatggtgg atctggacct gtgccaattc aatatggcgt atatggactc
      121 gtgccaattc aatatggtgg atctggaccc cagccaattc aatatggcgg acttggcacc
      181 atgccaattc aatatggcgg acctggcact gtgccaactg gggaggggtc tacttggcac
      241 ggtgccaagt ttgaggaggg gtcttggccc tgtgccaagt ccgccatatt gaattggcat
      301 ggtgccaata atggcggcca tattggctat atgccaggat caatatatag gcaatatcca
      361 atatggccct atgccaatat ggctattggc caggttcaat actatgtatt ggccctatgc
      421 catatagtat tccatatatg ggttttccta ttgacgtaga tagcccctcc caatgggcgg
      481 tcccatatac catatatggg gcttcctaat accgcccata gccactcccc cattgacgtc
      541 aatggtctct atatatggtc tttcctattg acgtcatatg ggcggtccta ttgacgtata
      601 tggcgcctcc cccattgacg tcaattacgg taaatggccc gcctggctca atgcccattg
      661 acgtcaatag gaccacccac cattgacgtc aatgggatgg ctcattgccc attcatatcc
      721 gttctcacgc cccctattga cgtcaatgac ggtaaatggc ccacttggca gtacatcaat
      781 atctattaat agtaacttgg caagtacatt actattggaa gtacgccagg gtacattggc
      841 agtactccca ttgacgtcaa tggcggtaaa tggcccgcga tggctgccaa gtacatcccc
      901 attgacgtca atggggaggg gcaatgacgc aaatgggcgt tccattgacg taaatgggcg
      961 gtaggcgtgc ctaatgggag gtctatataa gcaatgctcg tttagggaac cgccattctg
     1021 cctggggacg tcggagcaag cttgatttag gtgacactat agaatacaag ctacttgttc
     1081 tttttgcagg atccaacttc atctctgaag gcttccaccg tcttgacaaa actgcaagct
     1141 gtgacatgga cttcatcttc tctacccctc ccaacttaac ttgtcaatta atcagaattt
     1201 caaccattta aatttatggt tgaattagac ctgaagcagc catcaagaac ttcaaggaaa
     1261 acctcccagt tgctggaaat cctcgtgccg aattcgccac ccaggcctcg ctaccttagg
     1321 accgttatag ttactggccg tcgttttaca agcctgcttt tttgtacaaa cttgtggatc
     1381 ccttccagga ggttgtattg gaaagcagta gtgtggacga attgcgagag aagcttagtg
     1441 aaatcagtgg gattcctttg gatgatattg aatttgctaa gggtagagga acatttccct
     1501 gtgatatttc tgtccttgat attcatcaag atttagactg gaatcctaaa gtttctaccc
     1561 tgaatgtctg gcctctttat atctgtgatg atggtgcggt catattttat agggataaaa
     1621 cagaagaatt aatggaattg acagatgagc aaagaaatga actgatgaaa aaagaaagca
     1681 gtcgactcca gaagactgga catcgtgtaa catactcacc tcgtaaagag aaagcactaa
     1741 aaatatatct ggatggagca ccaaataaag atctgactca agactgactc tgatagtgta
     1801 gcattttccc tgggggagtt ttggttttaa ttagatggtt cactaccact gggtagtgcc
     1861 attttggccg gacatggttg gggtaaccca gtgacaccag cactgattgg actgccctac
     1921 accaatcaga agctcagtgc ccaatgggcc actgttttga ctcggaatca tgttgtgcac
     1981 tatagtcaaa tgtactgtaa agtgaaaagg gatgtgcaaa aaaataaaaa aaaacaacaa
     2041 aaaaagctaa ccttctatta gaaaagggga caggggaatg agtaaacttc ttttattgcg
     2101 gacaaatgtg cacatagccg ctagtaaaac tagcctcaaa caggatgctc atagcttaat
     2161 aataaaagct gtgcaaaggc catgaatgaa tgaattttct gtttatttca ctgatgcaca
     2221 cattacctca ttgacaattc agaagtaaat ccaacgtgtg ttgactcttg gaaagcagca
     2281 aaaacaggag ctgaagaaaa gaaattcttg gaaccagccg taacccagta aggaattgtg
     2341 aagttgtgtt tttattttgt ttcatttttt gcagagtatt aagaacatta ttctggaaca
     2401 tcagaacgtt tcccttagac cgatcccagc aggtggcagc tcagattgct gcagtgttgt
     2461 aattataact gattgtactt aagttatgga tgtagagaat atgtttcatt catttattca
     2521 gcatgtaaat aaaattgatc ctgttgagtt atcataattg cagttcaact atctgccatg
     2581 attattcttt tcacgtatca ttcattctgt acatttgtgt acattgagaa gtatagcaat
     2641 ctatgtaaat gtaatcctca gtgaggttcc tcagtgctag gtcccatagg attgtcgttg
     2701 cccttgttaa tgaggtttct ctgttcagcg gcttcaattt ttttctcttt gtacatctag
     2761 ttttgaagat ttacttcaag tttgaatctt ctagaatgct tgtaagtcca gttttaattt
     2821 ttagagtcaa tttgtagtta catgtagttt aacttttggg aaacgtctta acattgttct
     2881 gaataaactt gctaatgagg tcaggtcatg gtacagactg atgcagtcaa catgatttca
     2941 ttgcagagtt tattagtatc agcaagtttt tgctttgcta aataaaagta ctcaatgaac
     3001 acaattctac ataaattttg acataccatc taatttataa aaatcaataa aaaaggtttt
     3061 ggtaaaactt tttcatgcca gatgctgttt acaacaatga acatgccaat aaaacatttg
     3121 ttcattctgt tgtgttattt tagtcattaa acttctgtgg atgaagaatc tgggttaaga
     3181 atagatttgt catctttaaa tatgacattt tgtaatgtgt attggatatc tcatttctat
     3241 gataaaggta tatttacagt aaagttctca taagagaaat gaaaagctgt gttaatatct
     3301 aactttgggg aaccctgtca gtatttcaga tccgattttt accctttttt tcttataaga
     3361 aagataaaat tagaaaatac tgttagcaaa tgtggctctg ccatttgaat ataatcaccg
     3421 agaattccat gtcttaaaag tctcctggaa tccacaatga aaaaaaaaat cttttctaag
     3481 gtatttttct ggctaatttt tatttgaaga aagctatagc atttagcgaa atttgactga
     3541 agtaatgttc tgagtttgca ttagtgggat tggtgatgtt ctcagaagaa aattggaaac
     3601 acttgtgatg aattgtcttt cagatcactt agattttctg atgtaagagg acagctattt
     3661 ggttctgata caggcctgct tacttgggat gtagggttag taaatggggt ttctgcttta
     3721 aaggactgac ttgctatcgc acaaaagagg cagacttgta aacacaatgg gctttggagt
     3781 ttggtctgat tgggtttggt ttagtattcc tatgagcgta aatggtaaaa ttcttctgat
     3841 acccactctt tagactgtgc cttctgctct gttctttgtt ttatgtttaa ctgctgtttc
     3901 taattgcagg tgtattacag atacaaataa gagtaaagaa aatatatttc attatagaaa
     3961 agaaaaaatt aaaagcttct tgcttttcag tgcctgatag agtgaaaaca caaagttgca
     4021 ctttaataat ttcaataaaa gctaatctgt gtcagcctcc ctctgcttca gagagtcagg
     4081 tgagcatcca taacctaaca ggcagagccc tagcgatgtg gatcaagttt cctgagcccg
     4141 ggggcggtgg agcctcatga tctcttatct tttgaggctg aggcaggtca catgcaacaa
     4201 attgtgaccc tgctccccac aagtcatgca aaggttttga agagctttta ccgtggggca
     4261 gatgaacttg tgtcaaccat gcacaccctg tgagaaccaa gtacctgtgt ttctaaggcg
     4321 ggcactcaag gtgaggggtg cattctggcc aaagaaacaa aagctgtggt ttcaggacca
     4381 tgccgtgtgt agctgatctg tacgggacgt gtatgtaagg aagagcaatc atgatagata
     4441 agaacagtgt gtgaagcagc cctcacacta gagtgtttgg tcatctctta taatgtaagg
     4501 gaaggtactt taaaattctg ggaagatgcg atgaactcat gtcccagtca gaaaataatc
     4561 caatgaaata agcattggtt gccaggccac agttaggaat tgtattgtga tacatctaga
     4621 ggccaagaga gcaggagaga gctaccaact tacactgtgg tttaagctaa atgaccgcac
     4681 agcatcatag cattgcagtg ttgttactaa atctggaagt gacctgtgaa tgtatggaat
     4741 acaataaagt cttttattct ggttcaaaaa aaaaaaaaaa aaaaaactcg agggcgcgcc
     4801 atctagaccc tatagtgagt cgtattacgt agatccagac atgataagat acattgatga
     4861 gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga
     4921 tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg
     4981 cattcatttt atgtttcagg ttcaggggga ggtgtgggag gttttttaat tcgcggccgc
     5041 ggcgccaatg cattgggccc ggtacccagc ttttgttccc tttagtgagg gttaattgcg
     5101 cgcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5161 ccacacaaca tacgagccgg gagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5221 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5281 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5341 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5401 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5461 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5521 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5581 cgaaacccga caggattata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5641 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5701 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5761 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     5821 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     5881 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     5941 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6001 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6061 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6121 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6181 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6241 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6301 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6361 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6421 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6481 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6541 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6601 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6661 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6721 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6781 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     6841 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     6901 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     6961 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7021 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7081 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7141 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7201 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7261 gtgccaccta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
     7321 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
     7381 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
     7441 tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac
     7501 catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta
     7561 aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag
     7621 ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg
     7681 taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcccat tcgccattca
     7741 ggctgcgcaa ctgttgggaa gggcgatcgg
//
