LOCUS       sequence-427213-        1710 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression of improved GABA sensor (positive change in 
            fluorescence).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Kolb I, Hasseman JP, Matsumoto A, Arthur BJ, Zhang Y, Tsang A, Reep 
            D, Tsegaye G, Zheng J, Patel R, Looger LL, Marvin JS, Korff WL, 
            Yonehara K, Turner GC
  TITLE     iGABASnFR2: Improved genetically encoded protein sensors of GABA
  JOURNAL   bioRxiv 2025.03.25.644953
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.03.25.644953"
COMMENT     Sequence Label: pGP-RSET-iGABASnFR2-6xHis
FEATURES             Location/Qualifiers
     source          1..1710
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1044..1065
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1168..1189)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             1690..1707
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atgggagaaa gcatcaattt cgtgagctgg ggcggtagca cccaggatgc gcagaagcag
       61 gcctgggccg acccgttcag caaggccagc ggcattaccg tggtccagga tgggcccacc
      121 gactacggca aactcaaggc catggtcgaa agcggcaacg tgcagtggga cgtggtggat
      181 gtagaggccg acttcgcctt gcgcgccgcc gccgaaggcc tgctcgaacc cctggatttc
      241 tcggtgatcc agcgcgacaa gatcgatccg cggttcgtga gtgaccatgg agtgggggcc
      301 ttcctgtact cttacgtgct cggctacaac gaaggcaagc tcggagccag caagccccag
      361 gactggaccg cgctgttcga caccaagacc taccccggca aacgcgccct ctacaaatgg
      421 ccgagccctg gcgtgctcga actggccctg ctcgccgacg gcgtaccggc cgacaagctc
      481 tacccgctgg acctggaccg cgccttcaag aaactcgaca ccatcaagaa agacatcgtc
      541 tggtggggcg gcggtgcaca gtcgcagcag ctgctggcct ccggcgaagt cagcatgggc
      601 cagttctgga acggtcgcat ccacgccctg caagaagacg gcgcgccagt gggcgtgagc
      661 tggaagcaga acctggtgat ggccgacatc ctcgtcgtgc ctaaaggcac gaagaacaaa
      721 gccgccgcga tgaagttcct ggccagtgcc agcagcgcca aaggccagga cgacttttcc
      781 gccctgaccg cctatgctcc agtgaacatc gacagtgtgc agcgcctcga ccttgcgcag
      841 gtccggatca ccgccgacaa gcagaagaac ggcatcatgg cgaacttcaa gatccgccac
      901 aacgtggagg acggcagcgt gcagagcgcc gaccactacc agcagaacac ccccatcggc
      961 gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccgt gctgagcaaa
     1021 gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc
     1081 actctcggca tggacgagct gtacaagggc ggtaccggag ggagcatgag caagggcgag
     1141 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1201 aagttcagcg tgcgcggcga gggcgagggc gatgccacca acggcaagct gaccctgaag
     1261 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1321 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1381 tccgccatgc ccgaaggcta cgtccaggag cgcaccatca gcttcaagga cgacggcacc
     1441 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     1501 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactgg
     1561 aacgccaacc tggcaccgaa cctgccgacc gcctacgtca aggatcagat caccctcgat
     1621 ttcgcctact gggccaagaa cggtccggcc attgcgacac ggtggaatga atggctagtc
     1681 aaaggtagcc atcatcacca tcaccactag
//
