LOCUS       sequence-42723-e         770 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 770)
  AUTHORS   Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM
  TITLE     A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit
            protein translation depending upon cell context.
  JOURNAL   Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
  PUBMED    21518959
REFERENCE   2  (bases 1 to 770)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2011-06"; volume: "31(12)"; pages: "2513-27. Epub 
            2011 Apr 25"
COMMENT     Sequence Label: pcDNA3.1 - HA-KLF4 FL delta K5S
FEATURES             Location/Qualifiers
     source          1..770
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        40..58
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             127..153
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             127..153
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggctactaga gaacccactg cttactggct tatcgaaatt aatacgactc actataggga
       61 gacccaagct ggctagcgtt taaacttaag cttggtaccg agctcggatc cggcgagtct
      121 gacatgtacc cctacgacgt gcccgactac gccgctgtca gcgacgcgct gctcccatct
      181 ttctccacgt tcgcgtctgg cccggcggga agagagaaga cactgcgtca agcaggtgcc
      241 ccgaataacc gctggcggga ggagctctcc cacatgaagc gacttccccc agtgcttccc
      301 ggccgcccct atgacctggc ggcggcgacc gtggccacag acctggagag cggcggagcc
      361 ggtgcggctt gcggcggtag caacctggcg cccctacctc ggagagagac cgaggagttc
      421 aacgatctcc tggacctgga ctttattctc tccaattcgc tgacccatcc tccggagtca
      481 gtggccgcca ccgtgtcctc gtcagcgtca gcctcctctt cgtcgtcgcc gtcgagcagc
      541 ggccctgcca gcgcgccctc cacctgcagc ttcacctatc cgatccgggc cgggaacgac
      601 ccgggcgtgg cgccgggcgg cacgggcgga ggcctcctct atggcaggga gtccgctccc
      661 cctccgacgg ctcccttcaa cctggcggac atcaacgacg tgagcccctc gggcggcttc
      721 gtggccgagc tcctgcggcc agaattggac ccggtgtaca ttccgccgca
//
