LOCUS       sequence-427245-        1485 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  AAV vector with hSynapsin promoter and LoxFAS sites for Cre-Off 
            expression of GCaMP6f (GCaMP6f will not be expressed in neurons that
            express Cre recombinase).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Meng C, Zhou J, Papaneri A, Peddada T, Xu K, Cui G
  TITLE     Spectrally Resolved Fiber Photometry for Multi-component Analysis of
            Brain Circuits.
  JOURNAL   Neuron. 2018 May 16;98(4):707-717.e4. doi: 
            10.1016/j.neuron.2018.04.012. Epub 2018 May 3.
  PUBMED    29731250
REFERENCE   2  (bases 1 to 1485)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2018.04.012"; journalName: "Neuron"; date: 
            "2018-05-16- 16"; volume: "98"; issue: "4"; pages: "707-717.e4"
COMMENT     Sequence Label: pAAV-hSyn-FAS-GCaMP6f-WPRE
FEATURES             Location/Qualifiers
     source          1..1485
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(38..46,47..64,65..67,68..100,101..103,104..127,
                     128..154,155..211,212..217,218..487,488..505,506..940,
                     941..946,947..1387)
                     /codon_start=1
                     /product="improved fluorescent protein-based calcium sensor
                     (Chen et al., 2013)"
                     /label=GCaMP6f
                     /translation="MGSHHHHHHGMASMTGGQQMGRDLYDDDDKDLATMVDSSRRKWNK
                     TGHAVRAIGRLSSLENVYIKADKQKNGIKANFKIRHNIEDGGVQLAYHYQQNTPIGDGP
                     VLLPDNHYLSVQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYKGGTGGSMVSKGEE
                     LFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLT
                     YGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFKDDGNYKTRAEVKFEGDTLVNRIE
                     LKGIDFKEDGNILGHKLEYNLPDQLTEEQIAEFKEEFSLFDKDGDGTITTKELGTVMRS
                     LGQNPTEAELQDMINEVDADGDGTIDFPEFLTMMARKMKYRDTEEEIREAFGVFDKDGN
                     GYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK"
     regulatory      134..143
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     424..445
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(551..572)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(812..831)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ttcgtatata cctttctata cgaagttgtg gcgcgccatg ggttctcatc atcatcatca
       61 tcatggtatg gctagcatga ctggtggaca gcaaatgggt cgggatctgt acgacgatga
      121 cgataaggat ctcgccacca tggtcgactc atcacgtcgt aagtggaata agacaggtca
      181 cgcagtcaga gctataggtc ggctgagctc actcgagaac gtctatatca aggccgacaa
      241 gcagaagaac ggcatcaagg cgaacttcaa gatccgccac aacatcgagg acggcggcgt
      301 gcagctcgcc taccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
      361 cgacaaccac tacctgagcg tgcagtccaa actttcgaaa gaccccaacg agaagcgcga
      421 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
      481 gtacaagggc ggtaccggag ggagcatggt gagcaagggc gaggagctgt tcaccggggt
      541 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      601 cgagggtgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
      661 caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt
      721 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
      781 ctacatccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
      841 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
      901 ggaggacggc aacatcctgg ggcacaagct ggagtacaac ctgccggacc aactgactga
      961 agagcagatc gcagaattta aagaggaatt ctccctattt gacaaggacg gggatgggac
     1021 aataacaacc aaggagctgg ggacggtgat gcggtctctg gggcagaacc ccacagaagc
     1081 agagctgcag gacatgatca atgaagtaga tgccgacggt gacggcacaa tcgacttccc
     1141 tgagttcctg acaatgatgg caagaaaaat gaaatacagg gacacggaag aagaaattag
     1201 agaagcgttc ggtgtgtttg ataaggatgg caatggctac atcagtgcag cagagcttcg
     1261 ccacgtgatg acaaaccttg gagagaagtt aacagatgaa gaggttgatg aaatgatcag
     1321 ggaagcagac atcgatgggg atggtcaggt aaactacgaa gagtttgtac aaatgatgac
     1381 agcgaaggat aacggctagc acaacttcgt atataccttt ctatacgaag ttgtgaattc
     1441 gatatcaagc ttatcgataa tcaacctctg gattacaaaa tttgt
//
