LOCUS       sequence-42725-e         838 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 838)
  AUTHORS   Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM
  TITLE     A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit
            protein translation depending upon cell context.
  JOURNAL   Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
  PUBMED    21518959
REFERENCE   2  (bases 1 to 838)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2011-06"; volume: "31(12)"; pages: "2513-27. Epub 
            2011 Apr 25"
COMMENT     Sequence Label: pMIR-Report-Luc-KLF4-K5S
FEATURES             Location/Qualifiers
     source          1..838
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(59..262)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(88..108)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        263..566
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(687..706)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(688..706)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(718..736)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(719..736)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(756..772)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(756..772)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(769..791)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    780..796
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(804..810,811..828,829..834))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 ccgggccttt ctttatgttt ttggcgtctt ccatggtgga tccacggttc actaaaccag
       61 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
      121 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
      181 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
      241 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
      301 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
      361 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
      421 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
      481 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
      541 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
      601 ccgtaattga ttactattaa taactaagat ctggtacctt gaattcatgc ttctcctccc
      661 tttagtgagg gtaattctct ctctctccct atagtgagtc gtattaattc cttctcttct
      721 atagtgtcac ctaaatcgtt gcaattcgta atcatgtcat agctgtttcc tgtgtgaaat
      781 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcct
//
