LOCUS       sequence-427267-       13922 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  dual-enSERT-2.2 vector for site-specific integration of.a two-color 
            enhancer-reporter construct (human beta-globin promoter) into the 
            H11 locus (separated by a synthetic insulator). *See Comments Sect.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13922)
  AUTHORS   Hollingsworth EW, Liu TA, Alcantara JA, Chen CX, Jacinto SH, Kvon EZ
  TITLE     Rapid and quantitative functional interrogation of human enhancer 
            variant activity in live mice.
  JOURNAL   Nat Commun. 2025 Jan 6;16(1):409. doi: 10.1038/s41467-024-55500-7.
  PUBMED    39762235
REFERENCE   2  (bases 1 to 13922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2025-01-6"; volume: "16(1):409. doi"; pages: " 
            10.1038/s41467-024-55500-7"
COMMENT     Sequence Label: PCR4-HBGP::mCherry-SI-HBGP::eGFP-H11
FEATURES             Location/Qualifiers
     source          1..13922
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     1303..1319
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    1316..1450
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(join(1605..2318,2319..2321,2322..2324))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(1608..2318,2319..2321,2322..2324))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1650..1671)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     1999..2018
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2258..2279
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      2321..2330
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5530..6240
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(5675..5693)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     5943..5962
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    6389..6523
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6520..6536)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8947..8966)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8948..8966)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8973..8990)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8973..8989)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8982..9004)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             9127..9429
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     9308..9327
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     CDS             9778..10572
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             9778..10572
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(9832..9851)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     10442..10461
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     CDS             complement(join(10822..11613,11614..11682))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11445..11464
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11806..12394
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12295..12314
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12548..12565
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12682..12703
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12718..12723,12724..12741,12742..12748)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12756..12772
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12761..12783
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12780..12796
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12780..12796
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        12817..12835
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aaaaaaaaaa aaaaaagagt aaaggcatag tctaccacat ccactgaggt gtgttcttac
       61 agccaaagac tttttctcct gaggtctaag ggaacctcgt agtatgagtc tcatgacatg
      121 gaatgaattt cctgttttgt tttcttggcc ttagttaagc taagcagacc cactattctg
      181 caccactcat tagttaggat gctaacactc atttaagagg ttaatgattg aaaaggcatt
      241 ttcacagaaa ccatatggcg ctccagtggc tttcattagg tcatttgaga atccagattt
      301 tctcatcttc tctattcact acaggttgtt ggcagttttg gccagttagc tcaccttgaa
      361 aatggaaaca tgtctgacaa gagccttgag ctgaatatca tccagagtat ttgctagaga
      421 cagggtctta agtctcatta aattgcttag aagtgtgttt agtcctataa actatgtctc
      481 atttgtgtgt ccttcacaaa gagcgctagt gtcgatcatc aattagccta gcctataaag
      541 gtgacacaac ctggtcagtc cctctgtatg tctactattt ccccttctga tttctactat
      601 cttctcaaga actggttctt cagcttcctt tgggaaatgt cactttttaa tgatgtgtgt
      661 tttgcttatg tatatgtcta catactaccc atgtgcttgg tcactgcaga ggccagaaga
      721 cggtgtcagg tgcactggaa ctagagttaa tgacagatgt gagccatagg gtgctttgtt
      781 cacatctttc cagtcccaaa tgcgtctaaa cttatgtgat acatactaga ttaccacaaa
      841 attgctccaa agacaccttt ctccctctga gatgaatctc tggctggcct tgctctaggc
      901 aatcctgtgt tcaattcaag gactgaattt gattacatcg gtaatccagt gccccaggca
      961 tttctgcttt ttctgtaagg ttcttatccc ctggaagact gtttacgtat tcattttttt
     1021 ttgagactaa gtttcaggta gaaaaagctt gccttgaact tcactatata gggcttatct
     1081 tgaactcttg tgggtcttcc acctttcttc agttagcttc tgtacactgc cagacatgaa
     1141 aatcagatcc atttatagat agatggtcat gagccaccat gtgggtgtct aaaattgatc
     1201 tcaggacctc tgaaagacca gctagttctc ttaactgctg agccatctct ctagcgtgtc
     1261 tatacacatt taatatcccc ttgttccctt tctgcttgcc gctctagaac tagtggatcc
     1321 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     1381 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1441 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg
     1501 ggaggttttt taaagcaagt aaaacctcta cagatgtgat atggtgagtg ctgattatga
     1561 tcattactta tctagagctt agatcccccc tgccctagtt attactactt gtacagctcg
     1621 tccatgccga gagtgatccc ggcggcggtc acgaactcca gcaggaccat gtgatcgcgc
     1681 ttctcgttgg ggtctttgct cagggcggac tgggtgctca ggtagtggtt gtcgggcagc
     1741 agcacggggc cgtcgccgat gggggtgttc tgctggtagt ggtcggcgag ctgcacgctg
     1801 ccgtcctcga tgttgtggcg gatcttgaag ttcaccttga tgccgttctt ctgcttgtcg
     1861 gccatgatat agacgttgtg gctgttgtag ttgtactcca gcttgtgccc caggatgttg
     1921 ccgtcctcct tgaagtcgat gcccttcagc tcgatgcggt tcaccagggt gtcgccctcg
     1981 aacttcacct cggcgcgggt cttgtagttg ccgtcgtcct tgaagaagat ggtgcgctcc
     2041 tggacgtagc cttcgggcat ggcggacttg aagaagtcgt gctgcttcat gtggtcgggg
     2101 tagcggctga agcactgcac gccgtaggtc agggtggtca cgagggtggg ccagggcacg
     2161 ggcagcttgc cggtggtgca gatgaacttc agggtcagct tgccgtaggt ggcatcgccc
     2221 tcgccctcgc cggacacgct gaacttgtgg ccgtttacgt cgccgtccag ctcgaccagg
     2281 atgggcacca ccccggtgaa cagctcctcg cccttgctca ccatggtggc ggcgccgcgc
     2341 tctgcttctg gaaggctgcg ctcctcgacc tgcaggctag aagcaaatgt aagcaataga
     2401 tggctctgcc ctgactttta tgcccagccc accggtaccc tgcacatagc agggggttgg
     2461 agctgggtga gcactgtgct cctttgcaac ccaggccatt ctatgattct gtcattctaa
     2521 atctctcttt cagcctaaag ctttttcccc gtatcccccc aggtgtctgc aggctcaaag
     2581 ggcagcgaga agcgttcaga ggaaagcgat cccgtgccac cttccccgtg cccgggctgt
     2641 ccccgcacgc tgccggctcg gggatgcggg gggagcgtcg ggccggagcg gagccccggg
     2701 cggctcgctg ctgcccccta gcgggagagg gacgtaatta catccctggg ggctttgggg
     2761 ggggggctct ccccgtgagc tcccgcggac gcccccttcc aggaccttcc aggaaatctt
     2821 cgctattacg ccagctggcg aaaccctgca catagcaggg ggttggagct gggtgagcac
     2881 tgtgctcctt tgcaacccag gccattctat gattctgtca ttctaaatct ctctttcagc
     2941 ctaaagcttt ttccccgtat ccccccaggt gtctgcaggc tcaaagagca gcgagaagcg
     3001 ttcagaggaa agcgatcccg tgccaccttc cccgtgcccg ggctgtcccc gcacgctgcc
     3061 ggctcgggga tgcgggggga gcgccggacc ggagcggagc cccgggcggc tcgctgctgc
     3121 cccctagcgg gagaggaacg caattacagc cctgggggct ttgggggggg gctctcccca
     3181 tgagctcccg cggacgcccc cttccaggac cttccaggaa agatcatagg cctgcatcca
     3241 tcccagaagc taagacctga tggtaactgc tgaagcaata tttcgtttct tcataacatc
     3301 tctattattt cgtttcttca taacatctct attttaacat gttttcaaat gttagctcaa
     3361 cttccaacat atttaaataa tttattgtac gttctcttaa cactgtgtta taggactcaa
     3421 ttatccggta ggaaaagcac aaataattgg tagtgtccta gtttactttt tttttttgtc
     3481 gtattatctt gggtcatagg agtatcccct ctgtgaaaat caattaacaa tattcaacat
     3541 gctgatatta aaagaaaagc tttaaggttc caccgagatt tgaactcgga tcgctggatt
     3601 cagagtccag agtgctaacc attacaccat ggaacctccc tcgtgggggc tttcaagatt
     3661 gtgtacatta ctggacattc tattttgcgt gtcttccaca cccgtcactt aaaacgaaaa
     3721 caaaacaaag tctgctgttg gatatggtag acaccgttat cgagcacttg aaatatatga
     3781 attcagatgt attgccagct tctaaataca catcttcttt tgaaggaagg ctttgtacaa
     3841 acaaaaagac ttaaaatttc attaatattt ttagaatgtt gattatatgt taaaataaga
     3901 ttttggtata ttagggtaag taaaatagat ccgtgaaatt aatttcgctt gctttttatt
     3961 ctttgtaatg tggctaccag aaaatgtaaa ttcgataata cggctcgcgt ttgtactgtt
     4021 ttaaagtatg tttattaccc tccaccgtcg tttgctgtat tttgtggaaa tcaagtttct
     4081 gtatcaccta gctttaaaaa ctagtaacta tttttgttac cattttatgc gaagtgcggt
     4141 acccaaaagc aaagacatgc cgcccgaaca gggacttgaa ccctggaccc tcagattaaa
     4201 agtctgatgc tctaccgact gagctatccg ggctcttcac gagagcttta ctttttgctt
     4261 ataagagggt tctctatagg aaaagccagg cttgtagaac cgacagagga ttttatctgt
     4321 gcagcataga atattttggc acagatttgg aagcagcggg tgaagctcgc ctgctgctga
     4381 ttgagctttt tctgcctccc gttcttagag cccccgccga ggctgcgacg cagggactgt
     4441 accatagtag aggctggaac agtgcggcgc cggaaccggc cgcgcggggc cgctgcgggc
     4501 tatgggcttc tctgagaggt tcctccccag tccctagtgg cccagatccc ggacacctgg
     4561 gctcccgccc aggatcctgc aggcccaggg cggtcctgga gcggaaagaa tgccacgcgg
     4621 ggcattcaga ccctgtttgc cggcgctgta tttcgctttc ctgacctgcc ctactccaga
     4681 gcagagaatg aggtcgggcg tgccaaaggc tggggacaaa gagggccatg cgtgggggga
     4741 tcggtgtatg gggtgacagg gagctgttcc tgaccatttg acatgtgctg gaatgcgtgt
     4801 gcgactcacc tgcgtgtggc tgcagtcacg tgtgatttat gaccaagtgc aactctccac
     4861 tgtttagtga ttcacacgga tgattcacac gtaggaatgc aggtgccctg tgctcatctc
     4921 tgcctgggaa tggggaggaa cagatcttgg gggacattgg ggagtgggcg gacaagcact
     4981 ccagggcatc agtccgggcc gctgaccagg cggagggcag tttagaacaa tggctggccc
     5041 atagtaaatg ccgtgttagt gtgttagttg ctgttcttcc acgtcagaag aggcacagac
     5101 aaattaccac caggtggcgc tcagagtctg cggaggcatc acaacagccc tgaatttgaa
     5161 tcctgctctg ccactgccta gttgagacct tttactacct gactagctag ggggatgtgc
     5221 tgcaaggcga ttaattagaa caatggctgg cccatagtaa atgccgtgtt agtgtgttag
     5281 ttgctgttct tccacgtcag aagaggcaca gacaaattac caccaggtgg cgctcagagt
     5341 ctgcggaggc atcacaacag ccctgaattt gaatcctgct ctgccactgc ctagttgaga
     5401 ccttttacta cctgactagc tgcggccgcg ggctgggcat aaaagtcagg gcagagccat
     5461 ctattgctta catttgcttc tagcctgcag gtcgaggagc gcagccttcc agaagcagag
     5521 cgcggcgcca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     5581 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     5641 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     5701 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     5761 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     5821 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     5881 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     5941 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     6001 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     6061 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     6121 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     6181 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     6241 taataactag ggcagggggg atctaagctc tagataagta atgatcataa tcagccatat
     6301 cacatctgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     6361 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     6421 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     6481 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atccactagt tctagagcgg
     6541 cagtaagtag ttctctattg gcagtttgac acatcctgcc cttaccttac taccactgtt
     6601 ggctcagcag acacccagga taagtgcact agtgttcctt tcctgaccag tgggactgct
     6661 ttttccagat tcctcttgaa gctgaggagg cagtgagagc atgatggcat ctaatgagct
     6721 tggaagtacc agactgccct gatccacagc caggttttgc tgaaaagtga ccagtttgtc
     6781 ctcctccagt agagtgggca gctgaaggat tataatctac tgtcaagact tggaggcccc
     6841 tgcagtcaaa gtccaataga atattatgaa atggagaatg gcttatttta atctctatag
     6901 tggaattaaa atagcattta tggccccaga tccataatta atccaatgac tggtcaaatt
     6961 agcttgaacc tgactgaaaa acctgcaatc agtatagtat ctttcagaat gctttacatt
     7021 caatttataa taccagactt caagttgtaa gttaacaatt ttgagagaaa ctaatgcagc
     7081 agaggcaagg gaaagactta aattaatgtg accattaatt agttggagtc aggctggatt
     7141 aacattgtgc cagtaaattt cagaaaaatt actatatgac ttctctgtaa actttgattt
     7201 tgtagagtat aatattgatt ccttgatact tgccacaagc tactttcagg gttagtccag
     7261 cttaaactaa tgctatcaga actttatgtg cagaagaaat tccagaagtc ctaaggtaaa
     7321 attaaaacgt gaaaggaact catttagact gtcttagttc atggaagaaa taaacacagt
     7381 accagccata tcgttcacac acgtggcata tgtaactttt aataaaccaa aagaaaaagt
     7441 cccaaatatt caagtgaaaa aaatccaaac agttgacaca agcctaactg atagttactt
     7501 tatgtacagc tgtatgtata agttcaaaaa aagctaccct atttgtatgt acaaaagttt
     7561 atacacaggt ctgtacataa gggtctatac attttatttt ttcagaaccc ttaggtgtca
     7621 cctctagaag acaccaacac ttcattcaca tattttataa aagaaagact tccaggactg
     7681 acaatcttgt atcccttgta tttgaaccat gtaggttcat tcgatttaac agccttctgt
     7741 cataatgtag atcatggtag gtatgtggat cccctaaaca tcttcacata gtgtgctcca
     7801 atgtaaaagc ccattacaaa gagggaatga gagtcatgac cagggtgtca ccacaggtcc
     7861 aggtatgggt gagccagcct gccctcttca ctgtctatat tccagttcat ctacactgat
     7921 gactttggtg ggcatggagg gcagaggctg ctgtagcaca tctgagccaa accatttggc
     7981 aaggcccaca ggggagctgc tcctttggct ggtacgatgc tccagctggg agtgcatgtg
     8041 gggcatgccc gaccggctgg gtacattctg aggagtctga acgctgatag ctgctgcctg
     8101 ggaactagag cctggaggat gcagaactat ggagagaaga gtgtcagtgc agcaaagtag
     8161 ctctactcta cacctcaccc acagaacccc acaagtctac taagaccacc cttggatact
     8221 cactcccaag tcctgttctc agtattgaca atattttctg acctactttg cttgaatggt
     8281 ttgttacttt gggggatggg aagtctacca gaggatttaa ccaaaggctt cctaaagtgc
     8341 aaaatgcaac gtaagaaaat gcaacgtaag aaagcccacc aaggctgaca tgttcacatc
     8401 tcagcaaaaa ttctcaggct tataagaagt gaaaaacaga gctagagcat ggctggctcc
     8461 gactgtcctt gcagtagtaa taatccctga gactgcacag gtcagttcac ttcacctgtt
     8521 tttctagtga cacctaagaa acaaaggggc agtgataact attgctcctg gaaatctcag
     8581 aactttccag tgtgatcagc taacctggcc tatttctaaa ccttacctga gcgctgtagc
     8641 tgctgctgca tcaccgccaa atgcagaggg gtcccaggac gagggtttag gagagggtga
     8701 ctagcagcag agaccaaagg gtacaagggt tgacccagga gtgggccaga tagcccctgt
     8761 aaaggagcca tgtctatccc aggaggaagc acacctgatg gggagaaaga agaaactcat
     8821 gagttgtaga tgttcctaaa aatgtcaaag tttactactt ttgttgattt ggatacttac
     8881 aaggaggtgg atgtggtgaa cacttgccac catacatgta ccaggggcgc gcctaaattc
     8941 aattcgccct atagtgagtc gtattacaat tcactggccg tcgttttaca acgtcgtgac
     9001 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     9061 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctatac
     9121 gtacggcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     9181 gtacagagtg atattattga cacgccgggg cgacggatgg tgatccccct ggccagtgca
     9241 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     9301 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     9361 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     9421 ggaatataaa tgtcaggcat gagattatca aaaaggatct tcacctagat ccttttcacg
     9481 tagaaagcca gtccgcagaa acggtgctga ccccggatga atgtcagcta ctgggctatc
     9541 tggacaaggg aaaacgcaag cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg
     9601 cgatagctag actgggcggt tttatggaca gcaagcgaac cggaattgcc agctggggcg
     9661 ccctctggta aggttgggaa gccctgcaaa gtaaactgga tggctttctt gccgccaagg
     9721 atctgatggc gcaggggatc aagctctgat caagagacag gatgaggatc gtttcgcatg
     9781 attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc
     9841 tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg
     9901 caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa
     9961 gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc
    10021 gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat
    10081 ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg
    10141 cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc
    10201 gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag
    10261 catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc
    10321 gaggatctcg tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc
    10381 cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata
    10441 gcgttggcta cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc
    10501 gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac
    10561 gagttcttct gaattattaa cgcttacaat ttcctgatgc ggtattttct ccttacgcat
    10621 ctgtgcggta tttcacaccg catcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
    10681 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagat tatcaaaaag
    10741 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
    10801 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
    10861 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
    10921 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
    10981 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
    11041 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
    11101 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
    11161 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
    11221 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
    11281 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
    11341 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
    11401 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca
    11461 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
    11521 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
    11581 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
    11641 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
    11701 ttattgaagc atttatcagg gttattgtct catgaccaaa atcccttaac gtgagttttc
    11761 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
    11821 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
    11881 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
    11941 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
    12001 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
    12061 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
    12121 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
    12181 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
    12241 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
    12301 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
    12361 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
    12421 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
    12481 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
    12541 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
    12601 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
    12661 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
    12721 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
    12781 aggaaacagc tatgaccatg attacgccaa gctcagaatt aaccctcact aaagggacta
    12841 gtcctgcagg tttaaacgaa ttcgcgcgca ttgactgcag gctgagtgag tgggggtacg
    12901 taaggaccca aagctatgta gcctagcttc tcaaggtgtc taaagaaagg aagggtagat
    12961 taccaatgag cagagtttgg gcttctctat ttttaaagtt ttaaaatgtt tatatcaagg
    13021 caaggtttct gttgtccttg ctggtctgga actagattgg ccttgaccta agagatgatg
    13081 cgacttctgc ctcccaagtg ctaattaaag gtgtgagtca ccacgcttgc cttggtgttt
    13141 tctatttata ggttatatat tcatttttcc tattgcatgt cacttcccaa attacatctc
    13201 atctagtcat ctgtgcaccc aatcacaaat aggcataacc tggtagattt ccactaaaag
    13261 ttacttagag gaagtttgtg aatttattat tacaggcaag ccatggaaat gtgtgtagct
    13321 gttgcagttg gtttgaggag gtgtaagtgc atggtgtatt tggtagagag actaggctgc
    13381 ctagttcatt atgagaagat atgtacaaca caaacgaagt agggtctcag gttcaactgt
    13441 taagctttct tgcctcaggc tgctggagag ttgcactcgt acttattcac tcaattcatt
    13501 ctttggccag gtgggtggtg gtggtggtgg ttcacacctt tagtcatagt gttgaggagg
    13561 cagaggcaag aggttctgtg tttgaggcca gctgggtcta tagattgaat tctaggaaag
    13621 ccagggctac atagagaaac ctgttttcaa aagaaaaaag aaaaaaagaa aagacgaatg
    13681 gtgtatgtgt gtgtttgtca gtcatctgtc tggaattgaa tttgagatcc ccctgcctcc
    13741 caagtgtcct gattaaaggc atagtctata tagctgggag tggtggcgca cacttttaat
    13801 tctagcactt gagaggcaga ggcaggcgga tctctgagtt cgaggccagc ctggtcaaca
    13861 aagtgagttc caggacagcc aaggctacac agagaaaccc tgtctcgaaa aaacccaaaa
    13921 aa
//
