LOCUS       sequence-42727-e         895 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 895)
  AUTHORS   Lin CC, Liu LZ, Addison JB, Wonderlin WF, Ivanov AV, Ruppert JM
  TITLE     A KLF4-miRNA-206 autoregulatory feedback loop can promote or inhibit
            protein translation depending upon cell context.
  JOURNAL   Mol Cell Biol. 2011 Jun;31(12):2513-27. Epub 2011 Apr 25.
  PUBMED    21518959
REFERENCE   2  (bases 1 to 895)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2011-06"; volume: "31(12)"; pages: "2513-27. Epub 
            2011 Apr 25"
COMMENT     Sequence Label: pMIR-Report-Luc-KLF4-K5S-Mt206B
FEATURES             Location/Qualifiers
     source          1..895
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(57..260)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(86..106)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        261..564
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     complement(685..704)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(686..704)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(716..734)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(717..734)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(754..770)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(754..770)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(767..789)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    778..794
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(802..808,809..826,827..832))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    847..868
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gggcctttct ttatgttttt ggcgtcttcc atggtggatc cacggttcac taaaccagct
       61 ctgcttatat agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag
      121 ttgttacgac attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg
      181 tcaatggggt ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg
      241 tactgccaaa accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa
      301 gtaggaaagt cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca
      361 ttgacgtcaa tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca
      421 gtttaccgta aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg
      481 ggaacatacg tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg
      541 ccatttaccg taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc
      601 gtaattgatt actattaata actaagatct ggtaccttga attcatgctt ctcctccctt
      661 tagtgagggt aattctctct ctctccctat agtgagtcgt attaattcct tctcttctat
      721 agtgtcacct aaatcgttgc aattcgtaat catgtcatag ctgtttcctg tgtgaaattg
      781 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
      841 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccc gcttt
//
