LOCUS       sequence-427282-       12190 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral mammalian vector with firefly luciferase reporter for 
            dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12190)
  AUTHORS   Orr JC, Laali A, Durrenberger PF, Lazarus KA, El Mdawar MB, Janes 
            SM, Hynds RE
  TITLE     A lentiviral toolkit to monitor airway epithelial cell 
            differentiation using bioluminescence.
  JOURNAL   Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L587-L599. 
            doi: 10.1152/ajplung.00047.2024. Epub 2024 Aug 13.
  PUBMED    39137525
REFERENCE   2  (bases 1 to 12190)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajplung.00047.2024"; journalName: "Am J Physiol Lung Cell 
            Mol Physiol"; date: "2024-10-1- 1"; volume: "327"; issue: "4"; 
            pages: "L587-L599"
COMMENT     Sequence Label: pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2
FEATURES             Location/Qualifiers
     source          1..12190
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    291..412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(328..347)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     382..401
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      452..587
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(607..626)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(608..626)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(636..653)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(636..652)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(645..667)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      794..1249
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(881..900)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1091..1112
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1275..1379
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1380..1448,1449..2240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1598..1617)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2411..2999
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2900..2919
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3153..3170
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3287..3308
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3323..3328,3329..3346,3347..3353)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3361..3377
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3366..3388
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3385..3401
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3385..3401
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3420..3440
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3422..3440
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        3541..3920
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3921..4124
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             4139..4319
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4366..4491
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4984..5217
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5402..5446
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5713..5830
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      7561..7570
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7567..9219
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(7633..7651)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     enhancer        9306..9609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9610..9813
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             9842..10774
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             10817..11494
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMKKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(10997..11017)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     11409..11432
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    11501..12089
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(11554..11574)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(11972..11983)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(12176..12190,1..219)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
       61 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
      121 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
      181 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca tgtcatctta
      241 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
      301 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
      361 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
      421 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
      481 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
      541 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
      601 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
      661 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
      721 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
      781 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      841 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      901 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      961 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     1021 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     1081 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     1141 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     1201 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     1261 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     1321 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     1381 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     1441 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     1501 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     1561 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     1621 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     1681 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     1741 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     1801 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     1861 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     1921 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     1981 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     2041 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     2101 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     2161 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     2221 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     2281 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     2341 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     2401 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     2461 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     2521 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     2581 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     2641 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     2701 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     2761 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     2821 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     2881 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     2941 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     3001 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     3061 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     3121 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     3181 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     3241 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     3301 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     3361 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     3421 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttggcca ttgcatacgt
     3481 tgtatccata tcataatatg tacatttata ttggctcatg tccaacatta ccgccatgtt
     3541 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     3601 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     3661 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     3721 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     3781 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     3841 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     3901 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     3961 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     4021 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     4081 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccggg
     4141 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4201 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4261 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4321 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     4381 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     4441 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     4501 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     4561 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     4621 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     4681 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     4741 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     4801 aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg gaggaggaga
     4861 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     4921 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     4981 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     5041 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
     5101 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
     5161 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
     5221 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     5281 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     5341 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     5401 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     5461 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     5521 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     5581 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     5641 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
     5701 gtatcggtta acttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     5761 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     5821 tcaaaatttt atcgatcacg agactagcct cgagactgta ttttgtaagt aggttttttt
     5881 ttttttgcct tctaggcagt gctcatttgg ttaattatag gtagaaagag agatcattct
     5941 tcccaatata tgtgtgaaga aatgaatgtt ttgtctgata taaatttgta gtgttacaga
     6001 gtactgtcat attcttgcat actcaagatc aaattatatt atgggagttt tttggttttt
     6061 gtgtataaaa gaaggaagca ttattaccag taacctatgg gtcttatata tcagccaatg
     6121 accacgtcat cctttaagtg cataaatttt atggatgcaa aagtagaagt aaaggggagg
     6181 caaatccgaa gtcgtgaacg tatttgcaaa ctttgtttct gaaatgtatg ttctaatctc
     6241 ttctggcttc caattctctt ctaaactttg tgtcttctaa attttagaag aaatcacaat
     6301 tttcacttat agttatcttg gccacttaca acctttcaag caaaatagag gaaattgggt
     6361 ccctgagtgg ggggtggggg tgtaggatga tgtcaagaaa taaatccaac tgaattacaa
     6421 atactcagca gtaaagagag tattttaatg aatcagatga taaccatcag ggtgcatttc
     6481 atctccacta aatcttcgta ccaaggccag attctacatg aatgttggtt cgtatttatg
     6541 taaatgtatt tttaaaacaa aagccaattg atatcttatg ctttaatact tattccacta
     6601 atcatttacg tagatgcatc acgtgcagta atcattttta ttacctattc acaagcaaaa
     6661 atattagtat aaactgggta caataaaata gagaaaagaa atattcaaat agataagcgt
     6721 tttgttaaaa aaaaaaaaaa gaagaaagaa aggacacatt tatcaggatt cctatttccc
     6781 gtacataata tggatgtttg tttgtttttg taagttaacg ggaccggtgg tttaacttgt
     6841 tattgaaaca tgctcgaaaa aatcaggtag cttattttgt aattgcttgt tatgaaacca
     6901 ctggcatttc tctggggaaa ataagttaaa aactctttag ctatcaggca gtgggtttta
     6961 attttttata ttggttaaat gtaacagtgg atttgcgtac tctctcctaa tttctaactt
     7021 tgtgtaatca ttcttgaaac cccaaatcta gattttaaaa aagaagcctt ctaaaagttt
     7081 tcctgaagtt tacttttcag ttacaaagag taaaataact ttctgaaatg ccttctgtaa
     7141 atcgtggtgg tggtgcggtt tgtttgggga gatttgtttt gtttttaaaa gacagtgcac
     7201 tttcttatga aagagacagg gaaagtttta cctgtctgtc tcctgggttt gttttttttt
     7261 tctttctttc tttttctttt aaagattggt gataaggaat tctaactact taatgagatg
     7321 ggagaggcct cactccattg gagtggagga gtccaggtgg aagttgatgg attggacagg
     7381 taaagagaag agtcccgcct cctcatgcct atagttgggt atatattagg aaaccttaaa
     7441 ttatgtacag agagagaaag agagagaggg acttgagttc tgttatcttc ttaagtagat
     7501 tcatattggg atccagatct gcgatctaag taagcttggc attccggtac tgttggtaaa
     7561 gccaccatgg aagacgccaa aaacataaag aaaggcccgg cgccattcta tccgctggaa
     7621 gatggaaccg ctggagagca actgcataag gctatgaaga gatacgccct ggttcctgga
     7681 acaattgctt ttacagatgc acatatcgag gtggacatca cttacgctga gtacttcgaa
     7741 atgtccgttc ggttggcaga agctatgaaa cgatatgggc tgaatacaaa tcacagaatc
     7801 gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg tgttgggcgc gttatttatc
     7861 ggagttgcag ttgcgcccgc gaacgacatt tataatgaac gtgaattgct caacagtatg
     7921 ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg ggttgcaaaa aattttgaac
     7981 gtgcaaaaaa agctcccaat catccaaaaa attattatca tggattctaa aacggattac
     8041 cagggatttc agtcgatgta cacgttcgtc acatctcatc tacctcccgg ttttaatgaa
     8101 tacgattttg tgccagagtc cttcgatagg gacaagacaa ttgcactgat catgaactcc
     8161 tctggatcta ctggtctgcc taaaggtgtc gctctgcctc atagaactgc ctgcgtgaga
     8221 ttctcgcatg ccagagatcc tatttttggc aatcaaatca ttccggatac tgcgatttta
     8281 agtgttgttc cattccatca cggttttgga atgtttacta cactcggata tttgatatgt
     8341 ggatttcgag tcgtcttaat gtatagattt gaagaagagc tgtttctgag gagccttcag
     8401 gattacaaga ttcaaagtgc gctgctggtg ccaaccctat tctccttctt cgccaaaagc
     8461 actctgattg acaaatacga tttatctaat ttacacgaaa ttgcttctgg tggcgctccc
     8521 ctctctaagg aagtcgggga agcggttgcc aagaggttcc atctgccagg tatcaggcaa
     8581 ggatatgggc tcactgagac tacatcagct attctgatta cacccgaggg ggatgataaa
     8641 ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga aggttgtgga tctggatacc
     8701 gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg tgagaggtcc tatgattatg
     8761 tccggttatg taaacaatcc ggaagcgacc aacgccttga ttgacaagga tggatggcta
     8821 cattctggag acatagctta ctgggacgaa gacgaacact tcttcatcgt tgaccgcctg
     8881 aagtctctga ttaagtacaa aggctatcag gtggctcccg ctgaattgga atccatcttg
     8941 ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc ttcccgacga tgacgccggt
     9001 gaacttcccg ccgccgttgt tgttttggag cacggaaaga cgatgacgga aaaagagatc
     9061 gtggattacg tcgccagtca agtaacaacc gcgaaaaagt tgcgcggagg agttgtgttt
     9121 gtggacgaag taccgaaagg tcttaccgga aaactcgacg caagaaaaat cagagagatc
     9181 ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat tctagagtcg ggatatcatg
     9241 caggaattcg attaatagta atcaattacg gggtcattag ttcatagccc atatatggag
     9301 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc
     9361 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga
     9421 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat
     9481 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc
     9541 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct
     9601 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca
     9661 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat
     9721 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg
     9781 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag
     9841 catggcttcc aaggtgtacg accccgagca acgcaaacgc atgatcactg ggcctcagtg
     9901 gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc atcaactact atgattccga
     9961 gaagcacgcc gagaacgccg tgatttttct gcatggtaac gctgcctcca gctacctgtg
    10021 gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc atcatccctg atctgatcgg
    10081 aatgggtaag tccggcaaga gcgggaatgg ctcatatcgc ctcctggatc actacaagta
    10141 cctcaccgct tggttcgagc tgctgaacct tccaaagaaa atcatctttg tgggccacga
    10201 ctggggggct tgtctggcct ttcactactc ctacgagcac caagacaaga tcaaggccat
    10261 cgtccatgct gagagtgtcg tggacgtgat cgagtcctgg gacgagtggc ctgacatcga
    10321 ggaggatatc gccctgatca agagcgaaga gggcgagaaa atggtgcttg agaataactt
    10381 cttcgtcgag accatgctcc caagcaagat catgcggaaa ctggagcctg aggagttcgc
    10441 tgcctacctg gagccattca aggagaaggg cgaggttaga cggcctaccc tctcctggcc
    10501 tcgcgagatc cctctcgtta agggaggcaa gcccgacgtc gtccagattg tccgcaacta
    10561 caacgcctac cttcgggcca gcgacgatct gcctaagatg ttcatcgagt ccgaccctgg
    10621 gttcttttcc aacgctattg tcgagggagc taagaagttc cctaacaccg agttcgtgaa
    10681 ggtgaagggc ctccacttca gccaggagga cgctccagat gaaatgggta agtacatcaa
    10741 gagcttcgtg gagcgcgtgc tgaagaacga gcagggcagc ggcagcgacg aggttgacgg
    10801 cagcggcagc gaattcatgg cctcctccga gaacgtcatc accgagttca tgcgcttcaa
    10861 ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag atcgagggcg agggcgaggg
    10921 ccgcccctac gagggccaca acaccgtgaa gctgaaggtg accaagggcg gccccctgcc
    10981 cttcgcctgg gacatcctgt ccccccagtt ccagtacggc tccaaggtgt acgtgaagca
    11041 ccccgccgac atccccgact acaagaagct gtccttcccc gagggcttca agtgggagcg
    11101 cgtgatgaac ttcgaggacg gcggcgtggc gaccgtgacc caggactcct ccctgcagga
    11161 cggctgcttc atctacaagg tgaagttcat cggcgtgaac ttcccctccg acggccccgt
    11221 gatgaagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
    11281 cgtgctgaag ggcgagaccc acaaggccct gaagctgaag gacggcggcc actacctggt
    11341 ggagttcaaa tccatctaca tggccaagaa gcccgtgcag ctgcccggct actactacgt
    11401 ggacgccaag ctggacatca cctcccacaa cgaggactac accatcgtgg agcagtacga
    11461 gcgcaccgag ggccgccacc acctgttcct gtaggtcgac aatcaacctc tggattacaa
    11521 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
    11581 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
    11641 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
    11701 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
    11761 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
    11821 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
    11881 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
    11941 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
    12001 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
    12061 tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc tttaagacca
    12121 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
    12181 gggctaattc
//
