LOCUS       sequence-427283-       11589 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral mammalian vector with firefly luciferase reporter for 
            MUC5AC expression; renilla luciferase and dsRed2 reporter under CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11589)
  AUTHORS   Orr JC, Laali A, Durrenberger PF, Lazarus KA, El Mdawar MB, Janes 
            SM, Hynds RE
  TITLE     A lentiviral toolkit to monitor airway epithelial cell 
            differentiation using bioluminescence.
  JOURNAL   Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L587-L599. 
            doi: 10.1152/ajplung.00047.2024. Epub 2024 Aug 13.
  PUBMED    39137525
REFERENCE   2  (bases 1 to 11589)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajplung.00047.2024"; journalName: "Am J Physiol Lung Cell 
            Mol Physiol"; date: "2024-10-1- 1"; volume: "327"; issue: "4"; 
            pages: "L587-L599"
COMMENT     Sequence Label: pCCL-MUC5ACPromoter-FLuc-CMV-RLuc-dsRed2
FEATURES             Location/Qualifiers
     source          1..11589
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      441..450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             447..2099
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(513..531)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     enhancer        2186..2489
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2490..2693
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2722..3654
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             3697..4374
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMKKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(3877..3897)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     4289..4312
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    4381..4969
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4434..4454)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4852..4863)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5056..5289
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5361..5482
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5398..5417)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5452..5471
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5522..5657
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5677..5696)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5678..5696)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5706..5723)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5706..5722)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5715..5737)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5864..6319
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5951..5970)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6161..6182
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6345..6449
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6450..6518,6519..7310)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6668..6687)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7481..8069
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7970..7989
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8223..8240
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8357..8378
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8393..8398,8399..8416,8417..8423)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8431..8447
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8436..8458
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8455..8471
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8455..8471
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8490..8510
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8492..8510
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        8611..8990
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8991..9194
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             9209..9389
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9436..9561
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10054..10287
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10472..10516
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10783..10900
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ctgttctgag cactcacttc tgggcaccag gaactcacag gctgctgggc atggcacggt
       61 gcccagggag agtctagggt ggggtatgtg gggaggaccc ctgcaggcca gggcttgggg
      121 gggccctcgg aaactgggct ctacccggca gacacaccca tctccgcctg ccaccggccg
      181 ctggccagcc cgcagtgagc acccactgtt tacttgggtg agggggaacc acaggccccg
      241 ccctgcccac ccacgtgaag cacggggctg gagccagctc tggggctaca aaaagctcct
      301 gccaccttgg gtccctcctc agaggctgct gagggacagg gcactcttcc ccgccgtcca
      361 cacaatgagt gttggccgga ggaagctggg atccagatct gcgatctaag taagcttggc
      421 attccggtac tgttggtaaa gccaccatgg aagacgccaa aaacataaag aaaggcccgg
      481 cgccattcta tccgctggaa gatggaaccg ctggagagca actgcataag gctatgaaga
      541 gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag gtggacatca
      601 cttacgctga gtacttcgaa atgtccgttc ggttggcaga agctatgaaa cgatatgggc
      661 tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc tttatgccgg
      721 tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt tataatgaac
      781 gtgaattgct caacagtatg ggcatttcgc agcctaccgt ggtgttcgtt tccaaaaagg
      841 ggttgcaaaa aattttgaac gtgcaaaaaa agctcccaat catccaaaaa attattatca
      901 tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc acatctcatc
      961 tacctcccgg ttttaatgaa tacgattttg tgccagagtc cttcgatagg gacaagacaa
     1021 ttgcactgat catgaactcc tctggatcta ctggtctgcc taaaggtgtc gctctgcctc
     1081 atagaactgc ctgcgtgaga ttctcgcatg ccagagatcc tatttttggc aatcaaatca
     1141 ttccggatac tgcgatttta agtgttgttc cattccatca cggttttgga atgtttacta
     1201 cactcggata tttgatatgt ggatttcgag tcgtcttaat gtatagattt gaagaagagc
     1261 tgtttctgag gagccttcag gattacaaga ttcaaagtgc gctgctggtg ccaaccctat
     1321 tctccttctt cgccaaaagc actctgattg acaaatacga tttatctaat ttacacgaaa
     1381 ttgcttctgg tggcgctccc ctctctaagg aagtcgggga agcggttgcc aagaggttcc
     1441 atctgccagg tatcaggcaa ggatatgggc tcactgagac tacatcagct attctgatta
     1501 cacccgaggg ggatgataaa ccgggcgcgg tcggtaaagt tgttccattt tttgaagcga
     1561 aggttgtgga tctggatacc gggaaaacgc tgggcgttaa tcaaagaggc gaactgtgtg
     1621 tgagaggtcc tatgattatg tccggttatg taaacaatcc ggaagcgacc aacgccttga
     1681 ttgacaagga tggatggcta cattctggag acatagctta ctgggacgaa gacgaacact
     1741 tcttcatcgt tgaccgcctg aagtctctga ttaagtacaa aggctatcag gtggctcccg
     1801 ctgaattgga atccatcttg ctccaacacc ccaacatctt cgacgcaggt gtcgcaggtc
     1861 ttcccgacga tgacgccggt gaacttcccg ccgccgttgt tgttttggag cacggaaaga
     1921 cgatgacgga aaaagagatc gtggattacg tcgccagtca agtaacaacc gcgaaaaagt
     1981 tgcgcggagg agttgtgttt gtggacgaag taccgaaagg tcttaccgga aaactcgacg
     2041 caagaaaaat cagagagatc ctcataaagg ccaagaaggg cggaaagatc gccgtgtaat
     2101 tctagagtcg ggatatcatg caggaattcg attaatagta atcaattacg gggtcattag
     2161 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     2221 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2281 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2341 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2401 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2461 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2521 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2581 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2641 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     2701 tgaaccgtca gatccgctag catggcttcc aaggtgtacg accccgagca acgcaaacgc
     2761 atgatcactg ggcctcagtg gtgggctcgc tgcaagcaaa tgaacgtgct ggactccttc
     2821 atcaactact atgattccga gaagcacgcc gagaacgccg tgatttttct gcatggtaac
     2881 gctgcctcca gctacctgtg gaggcacgtc gtgcctcaca tcgagcccgt ggctagatgc
     2941 atcatccctg atctgatcgg aatgggtaag tccggcaaga gcgggaatgg ctcatatcgc
     3001 ctcctggatc actacaagta cctcaccgct tggttcgagc tgctgaacct tccaaagaaa
     3061 atcatctttg tgggccacga ctggggggct tgtctggcct ttcactactc ctacgagcac
     3121 caagacaaga tcaaggccat cgtccatgct gagagtgtcg tggacgtgat cgagtcctgg
     3181 gacgagtggc ctgacatcga ggaggatatc gccctgatca agagcgaaga gggcgagaaa
     3241 atggtgcttg agaataactt cttcgtcgag accatgctcc caagcaagat catgcggaaa
     3301 ctggagcctg aggagttcgc tgcctacctg gagccattca aggagaaggg cgaggttaga
     3361 cggcctaccc tctcctggcc tcgcgagatc cctctcgtta agggaggcaa gcccgacgtc
     3421 gtccagattg tccgcaacta caacgcctac cttcgggcca gcgacgatct gcctaagatg
     3481 ttcatcgagt ccgaccctgg gttcttttcc aacgctattg tcgagggagc taagaagttc
     3541 cctaacaccg agttcgtgaa ggtgaagggc ctccacttca gccaggagga cgctccagat
     3601 gaaatgggta agtacatcaa gagcttcgtg gagcgcgtgc tgaagaacga gcagggcagc
     3661 ggcagcgacg aggttgacgg cagcggcagc gaattcatgg cctcctccga gaacgtcatc
     3721 accgagttca tgcgcttcaa ggtgcgcatg gagggcaccg tgaacggcca cgagttcgag
     3781 atcgagggcg agggcgaggg ccgcccctac gagggccaca acaccgtgaa gctgaaggtg
     3841 accaagggcg gccccctgcc cttcgcctgg gacatcctgt ccccccagtt ccagtacggc
     3901 tccaaggtgt acgtgaagca ccccgccgac atccccgact acaagaagct gtccttcccc
     3961 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggc gaccgtgacc
     4021 caggactcct ccctgcagga cggctgcttc atctacaagg tgaagttcat cggcgtgaac
     4081 ttcccctccg acggccccgt gatgaagaag aagaccatgg gctgggaggc ctccaccgag
     4141 cgcctgtacc cccgcgacgg cgtgctgaag ggcgagaccc acaaggccct gaagctgaag
     4201 gacggcggcc actacctggt ggagttcaaa tccatctaca tggccaagaa gcccgtgcag
     4261 ctgcccggct actactacgt ggacgccaag ctggacatca cctcccacaa cgaggactac
     4321 accatcgtgg agcagtacga gcgcaccgag ggccgccacc acctgttcct gtaggtcgac
     4381 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4441 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     4501 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     4561 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4621 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4681 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4741 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4801 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4861 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4921 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     4981 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5041 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5101 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5161 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5221 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5281 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     5341 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     5401 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     5461 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     5521 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5581 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     5641 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     5701 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     5761 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     5821 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5881 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5941 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     6001 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     6061 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     6121 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     6181 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     6241 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     6301 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     6361 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     6421 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     6481 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     6541 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     6601 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     6661 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     6721 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     6781 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6841 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6901 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6961 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     7021 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     7081 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     7141 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     7201 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     7261 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     7321 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     7381 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     7441 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     7501 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     7561 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     7621 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     7681 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     7741 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     7801 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7861 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7921 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7981 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     8041 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     8101 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     8161 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     8221 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     8281 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     8341 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     8401 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     8461 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     8521 tggagctgca agcttggcca ttgcatacgt tgtatccata tcataatatg tacatttata
     8581 ttggctcatg tccaacatta ccgccatgtt gacattgatt attgactagt tattaatagt
     8641 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     8701 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     8761 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     8821 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     8881 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     8941 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     9001 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     9061 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     9121 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     9181 atataagcag agctcgttta gtgaaccggg gtctctctgg ttagaccaga tctgagcctg
     9241 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     9301 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     9361 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     9421 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     9481 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     9541 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     9601 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     9661 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     9721 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     9781 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     9841 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     9901 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     9961 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
    10021 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
    10081 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
    10141 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
    10201 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
    10261 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
    10321 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
    10381 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
    10441 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
    10501 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
    10561 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
    10621 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
    10681 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
    10741 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg
    10801 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    10861 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt atcgatcacg agactagcct
    10921 cgagactcac tgggaccttt ctgtgctggg actgctcgga ccagtcaaca gcttcctgtc
    10981 cagagggtac tgagcatttc tggatcttgg tggccagaga ccatcaagtg acttgaactg
    11041 gccctgcccg cctggggtca ggagacagaa gcacaggtgg actcctgggc aatgctggga
    11101 gggggctgca tggtgaggga ggggttctat catttgcctg gaggctgctg ccaggagccc
    11161 ctctccaggg agggtgaggc tggctggcgc tacttcagtg gcagcatgtg gctggcctga
    11221 gggacgcctt ggctcactca ctcctcaatc actcatttac tcattcattc actcactcaa
    11281 tcatttttcc tttcgccaca ctcctgccgc atgtgctctc tctctaggca tccgggtaag
    11341 acaagacatg gggagtaaaa ggcacagaaa tggagaaata ggtgaccata aggagctttg
    11401 gatggggctg gggctggcct ctccctccca ggcagccaca cattccccag ccaaggtcgg
    11461 cagctccact gcccatgtgg agggtccaac caggaggtcg gccatgggag gaatggcagg
    11521 aaagggaaag gctgggctgg gccccctgtc ctgctgcaga gggattagtg tcaaagaggt
    11581 gccttaagg
//
