LOCUS       sequence-427284-       11628 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral mammalian vector with firefly luciferase reporter for 
            FOXJ1 expression; renilla luciferase and dsRed2 reporter under CMV 
            promoter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11628)
  AUTHORS   Orr JC, Laali A, Durrenberger PF, Lazarus KA, El Mdawar MB, Janes 
            SM, Hynds RE
  TITLE     A lentiviral toolkit to monitor airway epithelial cell 
            differentiation using bioluminescence.
  JOURNAL   Am J Physiol Lung Cell Mol Physiol. 2024 Oct 1;327(4):L587-L599. 
            doi: 10.1152/ajplung.00047.2024. Epub 2024 Aug 13.
  PUBMED    39137525
REFERENCE   2  (bases 1 to 11628)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1152/ajplung.00047.2024"; journalName: "Am J Physiol Lung Cell 
            Mol Physiol"; date: "2024-10-1- 1"; volume: "327"; issue: "4"; 
            pages: "L587-L599"
COMMENT     Sequence Label: pCCL-FOXJ1Promoter-FLuc-CMV-RLuc-dsRed2
FEATURES             Location/Qualifiers
     source          1..11628
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      759..1347
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1248..1267
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1501..1518
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1635..1656
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1671..1676,1677..1694,1695..1701)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1709..1725
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1714..1736
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1733..1749
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1733..1749
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1768..1788
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1770..1788
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     enhancer        1889..2268
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2269..2472
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             2487..2667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2714..2839
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3332..3565
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3750..3794
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4061..4178
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      5347..5356
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5353..7005
                     /codon_start=1
                     /gene="luc+"
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="enhanced luc+ version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     primer_bind     complement(5419..5437)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     enhancer        7092..7395
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7396..7599
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             7628..8560
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     CDS             8603..9280
                     /codon_start=1
                     /product="improved tetrameric variant of DsRed fluorescent 
                     protein"
                     /label=DsRed2
                     /note="mammalian codon-optimized"
                     /translation="MASSENVITEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTVK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     ATVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMKKKTMGWEASTERLYPRDGVLKGETHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDAKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(8783..8803)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     9195..9218
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     misc_feature    9287..9875
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(9340..9360)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9758..9769)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9962..10195
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    10267..10388
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10304..10323)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10358..10377
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      10428..10563
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10583..10602)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10584..10602)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10612..10629)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10612..10628)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10621..10643)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10770..11225
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10857..10876)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11067..11088
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11251..11355
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11356..11424,11425..11628,1..588)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11574..11593)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
       61 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
      121 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
      181 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
      241 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
      301 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
      361 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
      421 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
      481 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
      541 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
      601 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
      661 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
      721 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
      781 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
      841 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
      901 actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
      961 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1021 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1081 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     1141 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     1201 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     1261 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     1321 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     1381 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     1441 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     1501 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg
     1561 cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt
     1621 gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc tttacacttt
     1681 atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac
     1741 agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg
     1801 gagctgcaag cttggccatt gcatacgttg tatccatatc ataatatgta catttatatt
     1861 ggctcatgtc caacattacc gccatgttga cattgattat tgactagtta ttaatagtaa
     1921 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
     1981 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
     2041 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
     2101 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt
     2161 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac
     2221 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
     2281 tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
     2341 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
     2401 cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
     2461 ataagcagag ctcgtttagt gaaccggggt ctctctggtt agaccagatc tgagcctggg
     2521 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2581 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     2641 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     2701 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     2761 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     2821 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     2881 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     2941 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     3001 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     3061 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     3121 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     3181 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     3241 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     3301 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     3361 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     3421 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     3481 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     3541 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     3601 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     3661 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     3721 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     3781 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     3841 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     3901 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     3961 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     4021 ccattcgatt agtgaacgga tctcgacggt atcggttaac ttttaaaaga aaagggggga
     4081 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4141 aagaattaca aaaacaaatt acaaaaattc aaaattttat cgatcacgag actagcctcg
     4201 agtgagccga gccgggactt agcggcgggt agggggttgg gtggcgcggg agagggcggc
     4261 agaaggaggg agttcgaaga gagttgccgc cagggagagt ggcaagtgcc cgcggtctcc
     4321 ttggagacgt gctggccaat ggcagaggcg gaagctgttt atgggggcgg agcgtcgcca
     4381 tggcagcagg agaagccttt ggagcggcta cttaatgccg gtcccggggc cgcggtagct
     4441 cagagaagcg ccgtcgggcg ggggggcggc aagaggcgct ggcagggccc ccgagctggg
     4501 ggcagagagc cgcagggggt gggagcaact tcttccagaa ccttcctctg gcccgggctg
     4561 cgctctgagc caggtaaggg atggggaggg ctgcaggcca gggggaaggg tgggcaacgg
     4621 taccaacccg ctctctcatt cgtccttaac ggaagtggtg ggcccaggga ctgacatcgg
     4681 agaagtctgg gggaaactcg cctcctgccc cccgttactg ttccgtcccc ttccttccca
     4741 atgatcccct atcataatat tccttttcaa ggatcagagg acctccaccg cccccgcccc
     4801 gtcgtaccgc cctcccaaac ttctcgcccc acttccattt gttgcgagga ggaagggggt
     4861 cctgatttcc tgctcttcgg gagaaaagga tagcctcgga tgcccctacc ctcttcattt
     4921 ggctaataaa acctcgaaga ggaaaggaag agggaggcag atctgatggg ggtgggaccc
     4981 cagggccttg cttctgtttc ccatacctcg gcactaaaag atcctcccca gctgcgggga
     5041 tgatgttaga gagggcgtcc ccgtacacac actgtcccct cgggggtggg gtagtgctca
     5101 gctcccaccg tggtcctcgc aggcaccaca tacttattcg gaggaggcgc ccccggggtc
     5161 gcgagatgga agcgcgcgtc cgggaggccg tgtaacggga gctgcgggac tcagcgggcc
     5221 agagagcgcg gcgggccacc cccggctcag cccgtggatg ctgaccgccc cctcggagag
     5281 tccccgcaga catgggatcc agatctgcga tctaagtaag cttggcattc cggtactgtt
     5341 ggtaaagcca ccatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg
     5401 ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt
     5461 cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac
     5521 ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac
     5581 agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta
     5641 tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac
     5701 agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt
     5761 ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg
     5821 gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt
     5881 aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg
     5941 aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc
     6001 gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg
     6061 attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg
     6121 atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc
     6181 cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc
     6241 aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc
     6301 gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc
     6361 aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat
     6421 gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg
     6481 gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg
     6541 attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga
     6601 tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac
     6661 cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc
     6721 atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac
     6781 gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa
     6841 gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt
     6901 gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga
     6961 gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaattcta gagtcgggat
     7021 atcatgcagg aattcgatta atagtaatca attacggggt cattagttca tagcccatat
     7081 atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
     7141 ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc
     7201 cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg
     7261 tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat
     7321 tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc
     7381 atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt
     7441 gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac
     7501 caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc
     7561 ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa ccgtcagatc
     7621 cgctagcatg gcttccaagg tgtacgaccc cgagcaacgc aaacgcatga tcactgggcc
     7681 tcagtggtgg gctcgctgca agcaaatgaa cgtgctggac tccttcatca actactatga
     7741 ttccgagaag cacgccgaga acgccgtgat ttttctgcat ggtaacgctg cctccagcta
     7801 cctgtggagg cacgtcgtgc ctcacatcga gcccgtggct agatgcatca tccctgatct
     7861 gatcggaatg ggtaagtccg gcaagagcgg gaatggctca tatcgcctcc tggatcacta
     7921 caagtacctc accgcttggt tcgagctgct gaaccttcca aagaaaatca tctttgtggg
     7981 ccacgactgg ggggcttgtc tggcctttca ctactcctac gagcaccaag acaagatcaa
     8041 ggccatcgtc catgctgaga gtgtcgtgga cgtgatcgag tcctgggacg agtggcctga
     8101 catcgaggag gatatcgccc tgatcaagag cgaagagggc gagaaaatgg tgcttgagaa
     8161 taacttcttc gtcgagacca tgctcccaag caagatcatg cggaaactgg agcctgagga
     8221 gttcgctgcc tacctggagc cattcaagga gaagggcgag gttagacggc ctaccctctc
     8281 ctggcctcgc gagatccctc tcgttaaggg aggcaagccc gacgtcgtcc agattgtccg
     8341 caactacaac gcctaccttc gggccagcga cgatctgcct aagatgttca tcgagtccga
     8401 ccctgggttc ttttccaacg ctattgtcga gggagctaag aagttcccta acaccgagtt
     8461 cgtgaaggtg aagggcctcc acttcagcca ggaggacgct ccagatgaaa tgggtaagta
     8521 catcaagagc ttcgtggagc gcgtgctgaa gaacgagcag ggcagcggca gcgacgaggt
     8581 tgacggcagc ggcagcgaat tcatggcctc ctccgagaac gtcatcaccg agttcatgcg
     8641 cttcaaggtg cgcatggagg gcaccgtgaa cggccacgag ttcgagatcg agggcgaggg
     8701 cgagggccgc ccctacgagg gccacaacac cgtgaagctg aaggtgacca agggcggccc
     8761 cctgcccttc gcctgggaca tcctgtcccc ccagttccag tacggctcca aggtgtacgt
     8821 gaagcacccc gccgacatcc ccgactacaa gaagctgtcc ttccccgagg gcttcaagtg
     8881 ggagcgcgtg atgaacttcg aggacggcgg cgtggcgacc gtgacccagg actcctccct
     8941 gcaggacggc tgcttcatct acaaggtgaa gttcatcggc gtgaacttcc cctccgacgg
     9001 ccccgtgatg aagaagaaga ccatgggctg ggaggcctcc accgagcgcc tgtacccccg
     9061 cgacggcgtg ctgaagggcg agacccacaa ggccctgaag ctgaaggacg gcggccacta
     9121 cctggtggag ttcaaatcca tctacatggc caagaagccc gtgcagctgc ccggctacta
     9181 ctacgtggac gccaagctgg acatcacctc ccacaacgag gactacacca tcgtggagca
     9241 gtacgagcgc accgagggcc gccaccacct gttcctgtag gtcgacaatc aacctctgga
     9301 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     9361 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     9421 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     9481 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     9541 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     9601 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     9661 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     9721 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     9781 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     9841 gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc ggtaccttta
     9901 agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga
     9961 ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct
    10021 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
    10081 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
    10141 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtagt
    10201 agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt
    10261 gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    10321 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    10381 gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa
    10441 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
    10501 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag
    10561 gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt
    10621 cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc
    10681 acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca
    10741 acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc
    10801 gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc
    10861 tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa
    10921 tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact
    10981 tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt
    11041 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
    11101 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
    11161 aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac
    11221 aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa
    11281 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat
    11341 tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg
    11401 gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa
    11461 gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt
    11521 gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt
    11581 ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgc
//
