LOCUS       sequence-427286-        1187 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CRY2olig-mCherry fused to a codon-optimised poly-GP 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1187)
  AUTHORS   Hodgson RE, Rayment JA, Huang WP, Sanchez Avila A, Ellis BCS, Lin 
            YH, Soni N, Hautbergue GM, Shelkovnikova TA
  TITLE     C9orf72 poly-PR forms anisotropic condensates causative of nuclear 
            TDP-43 pathology.
  JOURNAL   iScience. 2024 Sep 14;27(10):110937. doi: 
            10.1016/j.isci.2024.110937. eCollection 2024 Oct 18.
  PUBMED    39391721
REFERENCE   2  (bases 1 to 1187)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-09-14"; volume: "27(10):110937. doi"; pages: " 
            10.1016/j.isci.2024.110937. eCollection 2024 Oct 18"
COMMENT     Sequence Label: CRY2olig-GP
FEATURES             Location/Qualifiers
     source          1..1187
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(24..43)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(25..43)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(50..67)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(50..66)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(59..81)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(774..793)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     1044..1062
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gnnncatcta gagggccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
       61 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
      121 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
      181 agttgcgcag cctatacgta cggcagttta aggtttacac ctataaaaga gagagccgtt
      241 atcgtctgtt tgtggatgta caagtaaagc ggccgcttaa gggccaggtc ctggtccggg
      301 tcctgggccg ggaccagggc cgggtccagg gcctggtccg gggccagggc cagggccagg
      361 gcctgggcca ggtccgggtc ctggtccggg acctgggcct ggtcctggtc ctgggcctgg
      421 tccagggccg ggaccggggc ctgggccggg acctggaccg ggtccggggc caggtcctgg
      481 tccagggcca gggcccatct tgtacagctc gtccatgccg ccggtggagt ggcggccctc
      541 ggcgcgttcg tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccaactt
      601 gatgttgacg ttgtaggcgc cgggcagctg cacgggcttc ttggccttgt aggtggtctt
      661 gacctcagcg tcgtagtggc cgccgtcctt cagcttcagc ctctgcttga tctcgccctt
      721 cagggcgccg tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt
      781 ctgcattacg gggccgtcgg aggggaagtt ggtgccgcgc agcttcacct tgtagatgaa
      841 ctcgccgtcc tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt
      901 catcacgcgc tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc
      961 ggcggggtgc ttcacgtagg ccttggagcc gtacatgaac tgaggggaca ggatgtccca
     1021 ggccaaaggg cagggggcca cccttggtca ccttcagctt ggcggtctgg gtgccctcgt
     1081 aggggcggcc ctcgccctcg ccctcgatct cgaactcgtg gccgttcacg gaaccctcca
     1141 tgtgcacctt gaaacccatg aaactccttg aagaagggcc aggttat
//
