LOCUS       sequence-427287-        1231 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CRY2olig-mCherry fused to a codon-optimised poly-GR 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1231)
  AUTHORS   Hodgson RE, Rayment JA, Huang WP, Sanchez Avila A, Ellis BCS, Lin 
            YH, Soni N, Hautbergue GM, Shelkovnikova TA
  TITLE     C9orf72 poly-PR forms anisotropic condensates causative of nuclear 
            TDP-43 pathology.
  JOURNAL   iScience. 2024 Sep 14;27(10):110937. doi: 
            10.1016/j.isci.2024.110937. eCollection 2024 Oct 18.
  PUBMED    39391721
REFERENCE   2  (bases 1 to 1231)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-09-14"; volume: "27(10):110937. doi"; pages: " 
            10.1016/j.isci.2024.110937. eCollection 2024 Oct 18"
COMMENT     Sequence Label: CRY2olig-GR
FEATURES             Location/Qualifiers
     source          1..1231
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(214..921)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(489..508)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     758..776
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      918..927
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctnnnaggt ccgcggcctc tgcctcgtcc cctgccccgt ccccttcccc ttccacgtcc
       61 ccgccctcta ccccgtcctc ttcctcgccc tcggccgcgt cccctgcctc ggcctcgccc
      121 tcttcctctt ccgcgtcctc gtccccttcc tctacctctg cccctgccac gtcctcgacc
      181 acgtcctctg cctcttcccc ggcctctgcc catcttgtac agctcgtcca tgccgccggt
      241 ggagtggcgg ccctcggcgc gttcgtactg ttccacgatg gtgtagtcct cgttgtggga
      301 ggtgatgtcc aacttgatgt tgacgttgta ggcgccgggc agctgcacgg gcttcttggc
      361 cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg tccttcagct tcagcctctg
      421 cttgatctcg cccttcaggg cgccgtcctc ggggtacatc cgctcggagg aggcctccca
      481 gcccatggtc ttcttctgca ttacggggcc gtcggagggg aagttggtgc cgcgcagctt
      541 caccttgtag atgaactcgc cgtcctgcag ggaggagtcc tgggtcacgg tcaccacgcc
      601 gccgtcctcg aagttcatca cgcgctccca cttgaagccc tcggggaagg acagcttcaa
      661 gtagtcgggg atgtcggcgg ggtgcttcac gtaggccttg gagccgtaca tgaactgagg
      721 ggacaggatg tcccaggcga agggcagggg gccacccttg gtcaccttca gcttggcggt
      781 ctgggtgccc tcgtaggggc ggccctcgcc ctcgccctcg atctcgaact cgtggccgtt
      841 cacggagccc tccatgtgca ccttgaagcg catgaactcc ttgatgatgg ccatgttatc
      901 ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggctgctg ctccgatcat
      961 gatctgtgct ccacgggttc ttgaaatagc tttagctagt agctcacgag ctgtgtcgat
     1021 gtctacaatg ggtttcgcat agtttgttcc gagttccaca ccaaaagctt tgagtacggt
     1081 taaaggaccg tcccatggat gatggatcca ttcagttggc aatctcgcaa gctcgggaaa
     1141 ccattgcctt aagtaccccc cttcggggtc aaatttgggc gcccttgtaa ccggggattg
     1201 tccaaccaat taaaccccgg gtccttccgg g
//
