LOCUS       sequence-427289-        1206 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of CRY2olig-mCherry fused to a codon-optimised poly-PR 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1206)
  AUTHORS   Hodgson RE, Rayment JA, Huang WP, Sanchez Avila A, Ellis BCS, Lin 
            YH, Soni N, Hautbergue GM, Shelkovnikova TA
  TITLE     C9orf72 poly-PR forms anisotropic condensates causative of nuclear 
            TDP-43 pathology.
  JOURNAL   iScience. 2024 Sep 14;27(10):110937. doi: 
            10.1016/j.isci.2024.110937. eCollection 2024 Oct 18.
  PUBMED    39391721
REFERENCE   2  (bases 1 to 1206)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-09-14"; volume: "27(10):110937. doi"; pages: " 
            10.1016/j.isci.2024.110937. eCollection 2024 Oct 18"
COMMENT     Sequence Label: CRY2olig-PR
FEATURES             Location/Qualifiers
     source          1..1206
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(221..928)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(496..515)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     765..783
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      925..934
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ctnngntctt gggtcttggc cgtggcctag gtcgtggccg tggccgtggt cgaggtctag
       61 gcctcgggcg tggcctcggc ctaggtctag gacgaggtcg aggccttggc ctgggccttg
      121 gacgtggacg tggcctggga cggggccttg gccgaggtct tggccttggt cttggtctgg
      181 ggcgaggtct aggtctgggt ctaggccggg gtctgggcat cttgtacagc tcgtccatgc
      241 cgccggtgga gtggcggccc tcggcgcgtt cgtactgttc cacgatggtg tagtcctcgt
      301 tgtgggaggt gatgtccaac ttgatgttga cgttgtaggc gccgggcagc tgcacgggct
      361 tcttggcctt gtaggtggtc ttgacctcag cgtcgtagtg gccgccgtcc ttcagcttca
      421 gcctctgctt gatctcgccc ttcagggcgc cgtcctcggg gtacatccgc tcggaggagg
      481 cctcccagcc catggtcttc ttctgcatta cggggccgtc ggaggggaag ttggtgccgc
      541 gcagcttcac cttgtagatg aactcgccgt cctgcaggga ggagtcctgg gtcacggtca
      601 ccacgccgcc gtcctcgaag ttcatcacgc gctcccactt gaagccctcg gggaaggaca
      661 gcttcaagta gtcggggatg tcggcggggt gcttcacgta ggccttggag ccgtacatga
      721 actgagggga caggatgtcc caggcgaagg gcagggggcc acccttggtc accttcagct
      781 tggcggtctg ggtgccctcg taggggcggc cctcgccctc gccctcgatc tcgaactcgt
      841 ggccgttcac ggagccctcc atgtgcacct tgaagcgcat gaactccttg atgatggcca
      901 tgttatcctc ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gctgctgctc
      961 cgatcatgat ctgtgctcca cgggttcttg aaatagcttt agctagtagc tcacgagctg
     1021 tgtcgatgtc tacaatgggt ttcgcatagt ttgttccgag ttccacacca aaaagctttg
     1081 agtacggtta aaggaacgtc ccatggatga aggatccatt cagttggcaa tctcgcaagc
     1141 tcgggaagcc attgccttag gtactcacct tcggggtcaa atttggcgcc cttgtaaacc
     1201 ngggaa
//
