LOCUS       sequence-42734-e         890 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 890)
  TITLE     Other Plasmids
REFERENCE   2  (bases 1 to 890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: V7 XLT.GFPLT CS2+
FEATURES             Location/Qualifiers
     source          1..890
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        55..73
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     55..72
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             141..851
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(168..196)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     791..811
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 gcaatgctcg tttagggaac cgccattctg cctggggacg tcggagcaag cttgatttag
       61 gtgacactat agaatacaag ctacttgttc tttttgcagg atcccatcga ttcgaattca
      121 aggcctctcg agcctctaga atgagtaaag gagaactttt cactggagtt gtcccaattc
      181 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag
      241 gtgatgcaac atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg
      301 ttccatggcc aacacttgtc actactctca cttatggtgt tcaatgcttt tcaagatacc
      361 cagatcatat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
      421 aaagaactat atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg
      481 aaggtgatac ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa
      541 acattcttgg acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag
      601 acaaacaaaa gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa
      661 gcgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
      721 taccagacaa ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga
      781 gagaccacat ggtccttctt gagtttgtaa cagctgctgg gattacacat ggcatggatg
      841 aactatacaa ataataagta gatccagaca tgataagata cattgatgag
//
