LOCUS       sequence-427343-        8510 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  E coli Genetic Modification.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8510)
  AUTHORS   Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu 
            J, Jiang W, Yang S
  TITLE     High-Efficiency Scarless Genetic Modification in Escherichia coli by
            Using Lambda Red Recombination and I-SceI Cleavage.
  JOURNAL   Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 
            18.
  PUBMED    24747889
REFERENCE   2  (bases 1 to 8510)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2014-07-1"; volume: "80(13)"; pages: 
            "3826-3834. Epub 2014 Apr 18"
COMMENT     Sequence Label: pREDTKI
FEATURES             Location/Qualifiers
     source          1..8510
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(1..879)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        906..1190
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1113..1132
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             1217..1225
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             1234..1650
                     /codon_start=1
                     /gene="gam"
                     /product="inhibitor of the host RecBCD nuclease in the 
                     lambda Red system"
                     /label=Gam
                     /note="protects the double-stranded DNA substrate for 
                     recombination"
                     /translation="MDINTETEIKQKHSLTPFPVFLISPAFRGRYFHSYFRSSAMNAYY
                     IQDRLEAQSWARHYQQLAREEKEAELADDMEKGLPQHLFESLCIDHLQRHGASKKSITR
                     AFDDDVEFQERMAEHIRYMVETIAHHQVDIDSEV"
     CDS             1656..2441
                     /codon_start=1
                     /gene="bet"
                     /product="single-stranded DNA binding recombinase in the 
                     lambda Red system"
                     /label=Beta
                     /note="mediates pairing of a single-stranded DNA with a 
                     complementary target"
                     /translation="MSTALATLAGKLAERVGMDSVDPQELITTLRQTAFKGDASDAQFI
                     ALLIVANQYGLNPWTKEIYAFPDKQNGIVPVVGVDGWSRIINENQQFDGMDFEQDNESC
                     TCRIYRKDRNHPICVTEWMDECRREPFKTREGREITGPWQSHPKRMLRHKAMIQCARLA
                     FGFAGIYDKDEAERIVENTAYTAERQPERDITPVNDETMQEINTLLIALDKTWDDDLLP
                     LCSQIFRRDIRASSELTQAEAVKALGFLKQKAAEQKVAA"
     CDS             2438..3118
                     /codon_start=1
                     /gene="exo"
                     /product="5' to 3' double-stranded DNA exonuclease in the 
                     lambda Red system"
                     /label=Exo
                     /note="creates 3' single-stranded DNA tails"
                     /translation="MTPDIILQRTGIDVRAVEQGDDAWHKLRLGVITASEVHNVIAKPR
                     SGKKWPDMKMSYFHTLLAEVCTGVAPEVNAKALAWGKQYENDARTLFEFTSGVNVTESP
                     IIYRDESMRTACSPDGLCSDGNGLELKCPFTSRDFMKFRLGGFEAIKSAYMAQVQYSMW
                     VTRKNAWYFANYDPRMKREGLHYVVIERDEKYMASFDEIVPEFIEKMDEALAEIGFVFG
                     EQWR"
     terminator      3119..3363
                     /label=lambda tL3 terminator
                     /note="transcription terminator tL3 from phage lambda"
     primer_bind     complement(4099..4121)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4110..4126
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     CDS             complement(4395..5477)
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     5439..5458
                     /label=LacI-R
                     /note="LacI, reverse primer"
     promoter        complement(5478..5555)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     CDS             complement(5688..6638)
                     /codon_start=1
                     /gene="rep101 encoding an A56V mutation"
                     /product="temperature-sensitive version of the RepA protein
                     needed for replication with the pSC101 origin (Armstrong et
                     al., 1984)"
                     /label=Rep101(Ts)
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYVQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      6686..6908
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     CDS             complement(7560..8375)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(7560..8375)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8283..8302
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8367..8386
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaag tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggaattc gagctctaag gaggttataa aaaatggata ttaatactga aactgagatc
     1261 aagcaaaagc attcactaac cccctttcct gttttcctaa tcagcccggc atttcgcggg
     1321 cgatattttc acagctattt caggagttca gccatgaacg cttattacat tcaggatcgt
     1381 cttgaggctc agagctgggc gcgtcactac cagcagctcg cccgtgaaga gaaagaggca
     1441 gaactggcag acgacatgga aaaaggcctg ccccagcacc tgtttgaatc gctatgcatc
     1501 gatcatttgc aacgccacgg ggccagcaaa aaatccatta cccgtgcgtt tgatgacgat
     1561 gttgagtttc aggagcgcat ggcagaacac atccggtaca tggttgaaac cattgctcac
     1621 caccaggttg atattgattc agaggtataa aacgaatgag tactgcactc gcaacgctgg
     1681 ctgggaagct ggctgaacgt gtcggcatgg attctgtcga cccacaggaa ctgatcacca
     1741 ctcttcgcca gacggcattt aaaggtgatg ccagcgatgc gcagttcatc gcattactga
     1801 tcgttgccaa ccagtacggc cttaatccgt ggacgaaaga aatttacgcc tttcctgata
     1861 agcagaatgg catcgttccg gtggtgggcg ttgatggctg gtcccgcatc atcaatgaaa
     1921 accagcagtt tgatggcatg gactttgagc aggacaatga atcctgtaca tgccggattt
     1981 accgcaagga ccgtaatcat ccgatctgcg ttaccgaatg gatggatgaa tgccgccgcg
     2041 aaccattcaa aactcgcgaa ggcagagaaa tcacggggcc gtggcagtcg catcccaaac
     2101 ggatgttacg tcataaagcc atgattcagt gtgcccgtct ggccttcgga tttgctggta
     2161 tctatgacaa ggatgaagcc gagcgcattg tcgaaaatac tgcatacact gcagaacgtc
     2221 agccggaacg cgacatcact ccggttaacg atgaaaccat gcaggagatt aacactctgc
     2281 tgatcgccct ggataaaaca tgggatgacg acttattgcc gctctgttcc cagatatttc
     2341 gccgcgacat tcgtgcatcg tcagaactga cacaggccga agcagtaaaa gctcttggat
     2401 tcctgaaaca gaaagccgca gagcagaagg tggcagcatg acaccggaca ttatcctgca
     2461 gcgtaccggg atcgatgtga gagctgtcga acagggggat gatgcgtggc acaaattacg
     2521 gctcggcgtc atcaccgctt cagaagttca caacgtgata gcaaaacccc gctccggaaa
     2581 gaagtggcct gacatgaaaa tgtcctactt ccacaccctg cttgctgagg tttgcaccgg
     2641 tgtggctccg gaagttaacg ctaaagcact ggcctgggga aaacagtacg agaacgacgc
     2701 cagaaccctg tttgaattca cttccggcgt gaatgttact gaatccccga tcatctatcg
     2761 cgacgaaagt atgcgtaccg cctgctctcc cgatggttta tgcagtgacg gcaacggcct
     2821 tgaactgaaa tgcccgttta cctcccggga tttcatgaag ttccggctcg gtggtttcga
     2881 ggccataaag tcagcttaca tggcccaggt gcagtacagc atgtgggtga cgcgaaaaaa
     2941 tgcctggtac tttgccaact atgacccgcg tatgaagcgt gaaggcctgc attatgtcgt
     3001 gattgagcgg gatgaaaagt acatggcgag ttttgacgag atcgtgccgg agttcatcga
     3061 aaaaatggac gaggcactgg ctgaaattgg ttttgtattt ggggagcaat ggcgatgacg
     3121 catcctcacg ataatatccg ggtaggcgca atcactttcg tctactccgt tacaaagcga
     3181 ggctgggtat ttcccggcct ttctgttatc cgaaatccac tgaaagcaca gcggctggct
     3241 gaggagataa ataataaacg aggggctgta tgcacaaagc atcttctgtt gagttaagaa
     3301 cgagtatcga gatggcacat agccttgctc aaattggaat caggtttgtg ccaataccag
     3361 tagaaacaga cgaagaatcc atggttatta tttcaggaaa gtttcggagg agatagtgtt
     3421 cggcagtttg tacatcatct gcgggatcag gtacggtttg atcaggttgt agaagatcag
     3481 gtaagacata gaatcgatgt agatgatcgg tttgtttttg ttgattttta cgtaacagtt
     3541 cagttggaat ttgttacgca gacccttaac caggtattct acttcttcga aagtgaaaga
     3601 ctgggtgttc agtacgatcg atttgttggt agagtttttg ttgtaatccc atttaccacc
     3661 atcatccatg aaccagtatg ccagagacat cggggtcagg tagttttcaa ccaggttgtt
     3721 cgggatggtt tttttgttgt taacgatgaa caggttagcc agtttgttga aagcttggtg
     3781 tttgaaagtc tgggcgcccc aggtgattac caggttaccc aggtggttaa cacgttcttt
     3841 tttgtgcggc ggggacagta cccactgatc gtacagcaga catacgtggt ccatgtatgc
     3901 tttgtttttc cactcgaact gcatacagta ggttttacct tcatcacgag aacggatgta
     3961 agcatcaccc aggatcagac cgatacctgc ttcgaactgt tcgatgttca gttcgatcag
     4021 ctgggatttg tattctttca gcagtttaga gttcggaccc aggttcatta cctggttttt
     4081 ttgatgcata tgctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     4141 cgagccggat gattaattgt caacagctca tttcagaata tttgccagaa ccgttatgat
     4201 gtcggcgcaa aaaacattat ccagaacggg agtgcgcctt gagcgacacg aattatgcag
     4261 tgatttacga cctgcacagc cataccacag cttccgatgg ctgcctgacg ccagaagcat
     4321 tggtgcaccg tgcagtcgat gataagctgt caaaccagat caattcgcgc taacttacat
     4381 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     4441 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt
     4501 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt
     4561 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt
     4621 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc
     4681 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg
     4741 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga
     4801 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga
     4861 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc
     4921 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta
     4981 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat
     5041 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga
     5101 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag
     5161 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg
     5221 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca
     5281 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa
     5341 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc
     5401 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat
     5461 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc
     5521 ataccgcgaa aggttttgca ccattcgatg gtgtcaacgt aaatgcatgc cgctcgcctt
     5581 ccatgggtat ggacagtttt ccctttgata tgtaacggtg aacagttgtt ctacttttgt
     5641 ttgttagtct tgatgcttca ctgatagata caagagccat aagaacctca gatccttccg
     5701 tatttagcca gtatgttctc tagtgtggtt cgttgttttt gcgtgagcca tgagaacgaa
     5761 ccattgagat catacttact ttgcatgtca ctcaaaaatt ttgcctcaaa actggtgagc
     5821 tgaatttttg cagttaaagc atcgtgtagt gtttttctta gtccgttatg taggtaggaa
     5881 tctgatgtaa tggttgttgg tattttgtca ccattcattt ttatctggtt gttctcaagt
     5941 tcggttacga gatccatttg tctatctagt tcaacttgga aaatcaacgt atcagtcggg
     6001 cggcctcgct tatcaaccac caatttcata ttgctgtaag tgtttaaatc tttacttatt
     6061 ggtttcaaaa cccattggtt aagcctttta aactcatggt agttattttc aagcattaac
     6121 atgaacttaa attcatcaag gctaatctct atatttgcct tgtgagtttt cttttgtgtt
     6181 agttctttta ataaccactc ataaatcctc atagagtatt tgttttcaaa agacttaaca
     6241 tgttccagat tatattttat gaattttttt aactggaaaa gataaggcaa tatctcttca
     6301 ctaaaaacta attctaattt ttcgcttgag aacttggcat agtttgtcca ctggaaaatc
     6361 tcaaagcctt taaccaaagg attcctgatt tccacagttc tcgtcatcag ctctctggtt
     6421 gctttagcta atacaccata agcattttcc ctactgatgt tcatcatctg aacgtattgg
     6481 ttataagtga acgataccgt ccgttctttc cttgtagggt tttcaatcgt ggggttgagt
     6541 agtgccacac agcataaaat tagcttggtt tcatgctccg ttaagtcata gcgactaatc
     6601 gctagttcat ttgctttgaa aacaactaat tcagacatac atctcaattg gtctaggtga
     6661 ttttaatcac tataccaatt gagatgggct agtcaatgat aattactagt ccttttcctt
     6721 tgagttgtgg gtatctgtaa attctgctag acctttgctg gaaaacttgt aaattctgct
     6781 agaccctctg taaattccgc tagacctttg tgtgtttttt ttgtttatat tcaagtggtt
     6841 ataatttata gaataaagaa agaataaaaa aagataaaaa gaatagatcc cagccctgtg
     6901 tataactcac tactttagtc agttccgcag tattacaaaa ggatgtcgca aacgctgttt
     6961 gctcctctac aaaacagacc ttaaaaccct aaaggcttaa gtagcaccct cgcaagctcg
     7021 gttgcggccg caatcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc
     7081 tgagtcgctg tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg
     7141 ggggtaaatg gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac
     7201 aagaaaagcc cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta
     7261 tctgactttt tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga
     7321 ttatcccgtg acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca
     7381 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7441 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7501 agtatatatg agtaaacttg gtctgacagt tacgtttcca accaattaac caattctgat
     7561 tagaaaaact catcgagcat caaatgaaac tgcaatttat tcatatcagg attatcaata
     7621 ccatattttt gaaaaagccg tttctgtaat gaaggagaaa actcaccgag gcagttccat
     7681 aggatggcaa gatcctggta tcggtctgcg attccgactc gtccaacatc aatacaacct
     7741 attaatttcc cctcgtcaaa aataaggtta tcaagtgaga aatcaccatg agtgacgact
     7801 gaatccggtg agaatggcaa aagcttatgc atttctttcc agacttgttc aacaggccag
     7861 ccattacgct cgtcatcaaa atcactcgca tcaaccaaac cgttattcat tcgtgattgc
     7921 gcctgagcga gacgaaatac gcgatcgctg ttaaaaggac aattacaaac aggaatcgaa
     7981 tgcaaccggc gcaggaacac tgccagcgca tcaacaatat tttcacctga atcaggatat
     8041 tcttctaata cctggaatgc tgttttcccg gggatcgcag tggtgagtaa ccatgcatca
     8101 tcaggagtac ggataaaatg cttgatggtc ggaagaggca taaattccgt cagccagttt
     8161 agtctgacca tctcatctgt aacatcattg gcaacgctac ctttgccatg tttcagaaac
     8221 aactctggcg catcgggctt cccatacaat cgatagattg tcgcacctga ttgcccgaca
     8281 ttatcgcgag cccatttata cccatataaa tcagcatcca tgttggaatt taatcgcggc
     8341 ctcgagcaag acgtttcccg ttgaatatgg ctcataacac cccttgtatt actgtttatg
     8401 taagcagaca gttttattgt tcatgatgat atatttttat cttgtgcaat gtaacatcag
     8461 agattttgag acacaacgtg gctttccccc cccccctgca gaaacattta
//
