LOCUS       sequence-427344-        4497 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Destination vector for cloning and transformation into TSI locus 1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4497)
  AUTHORS   Darino M, Urban M, Kaur N, Machado Wood A, Grimwade-Mann M, Smith D,
            Beacham A, Hammond-Kosack K
  TITLE     Identification and functional characterisation of a locus for target
            site integration in Fusarium graminearum.
  JOURNAL   Fungal Biol Biotechnol. 2024 Feb 26;11(1):2. doi: 
            10.1186/s40694-024-00171-8.
  PUBMED    38409036
REFERENCE   2  (bases 1 to 4497)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal Biol
            Biotechnol."; date: "2024-02-26"; volume: "11(1):2. doi"; pages: " 
            10.1186/s40694-024-00171-8"
COMMENT     Sequence Label: Fg vector
FEATURES             Location/Qualifiers
     source          1..4497
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(2631..3219)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2711..2730)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(3390..4178)
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      join(4469..4497,1..407)
                     /label=pSa ori
                     /note="origin of replication from bacterial plasmid pSa"
ORIGIN
        1 tttttatccc cggaagcctg tggatagagg gtagttatcc acgtgaaacc gctaatgccc
       61 cgcaaagcct tgattcacgg ggctttccgg cccgctccaa aaactatcca cgtgaaatcg
      121 ctaatcaggg tacgtgaaat cgctaatcgg agtacgtgaa atcgctaata aggtcacgtg
      181 aaatcgctaa tcaaaaaggc acgtgagaac gctaatagcc ctttcagatc aacagcttgc
      241 aaacacccct cgctccggca agtagttaca gcaagtagta tgttcaatta gcttttcaat
      301 tatgaatata tatatcaatt attggtcgcc cttggcttgt ggacaatgcg ctacgcgcac
      361 cggctccgcc cgtggacaac cgcaagcggt tgcccaccgt cgagcgcctt tgcccacaac
      421 ccggcggccg gccgcaacag atcgttttat aaattttttt ttttgaaaaa gaaaaagccc
      481 gaaaggcggc aacctctcgg gcttctggat ttccgatccc cggaattaaa cacgatgtcc
      541 tggtagcgat ccgctacgcc aagacgaccg caatcgatga aacctgagaa gcgaccgttc
      601 tcgaccatga tgttgggaag gcatgcatcg ccgtgcgtga cgaccagatc ctcaccgtct
      661 ggcatgcggg ccttcagacg tgcaaagagt tcggcgggcg ccaagccctg gtgttcctcg
      721 tcgagatcat cctggtccac aagacctgcc tccatacgtg ttcgagcgcg ttcgatgcga
      781 tgcttcgcct ggtgatcaaa cgggcacgtg gccggatcaa gggtgtgaag gcgacgcatc
      841 gcgtcggcca taatcgagac tttttctgcc ggggcgagat gcgaagacag caggtcttga
      901 cccggaacct ctcccaggag gagccaatca cggccagcct ctgtgaccac gtcgaggacc
      961 gcagcacacg gcacacccgt tgttgccaac caggagaggc gggctgcctc atcctgcaac
     1021 tcgttcaggg cgccggagag gtctgtctta acgaacagga ctggtcggcc ttgagcgctc
     1081 agacggaaga cggccgcgtc cgagcaacca attgtctgct gcgcccaatc atagccgaag
     1141 aggcgctcta cccaagcggc aggggagccg gcgtggagtc cgtcctgctc gatcatacag
     1201 cctgttggta ccagtcgact gaatactgtt tttgaagatt gggttcctct ttgagagagt
     1261 taatagactt gaattgagga atggaaatcg ggctgagctc actgctttat atgtgagaaa
     1321 aggtgatgag caggtggtaa gaggcagatt tttgtggtcg gtgctgtgtt ttgttgggtt
     1381 tgagctaggt ggggtgaggg aggggcaagc taaatagcta ccagccctga ggacgtcaca
     1441 gagttttagt agttcttttt ctgccatcgg acatgcagct cacgcctaga cccaattaca
     1501 ccctttgcgc cctcccacac atccggccgg ttggatcatt gtcagatacg gcagagaaat
     1561 cgcaacctcg gccaagggta gccacgattc gaagccgcga aattgggggt ttcggaatgt
     1621 cgtcaagcgg gaaccaattt gagtacccaa ttcgccctat ccaacatggt ggagtgagga
     1681 ccttgagacc ccgagggtct ctactaagac ccaaggacag gttgctttgc ttgatgtgtg
     1741 gtgggaatat tgcccagggc gtaattacct tgtttcgcga tttatcggtt gacgtctccc
     1801 tagttaagag gtagtttatt tgtcgtacat gtgtatctgt attaccagca ttccccagtg
     1861 ccagtcattt accgcgagac atgccttgtc aggggtgctt tgatatgttg acgggctccc
     1921 ggaggaaaga acacgagctc gactatctct catcgtctca tgttggtatg gagacaggcg
     1981 accccccccc cctgtgcagt aaataaactt tcgcttcatg tctgcatcgg gctcaaactc
     2041 aaggttcgtt gcatggattg agcgtatagg cgagtctccg cgaaacaagc ggatttctag
     2101 aagatacgga gtatgcgagt cagattgacg tttcacccca tcatgactgt aactactctg
     2161 catcgagccc gcaatgtccg attcatggtc tgtatacggt gcttgaccgg ctcttttgtt
     2221 gagattattt ctcggagctc atataggccc aagcttggtg ggatttccgt gggccgcgaa
     2281 atgattttct gtgttagcct acacaggact acttggccat attctccgcc aggatttctt
     2341 gcctttatgc ttccttcttt tttccctctt ccctgtttgc tccttttatg ccattttctt
     2401 tccctattga cctgaatggc gacgtgtcag atggaaaaca ccataccctt gcggggagtg
     2461 caggagtaca ttgtgcatat gttcaatggc tgaaatcctt ccaatgatat ccgtgcgatt
     2521 tggtaaattt gcagcccaaa cagttattgg tggttctggt caggagatct catgtgagca
     2581 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     2641 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     2701 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     2761 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     2821 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     2881 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     2941 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     3001 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     3061 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaga
     3121 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     3181 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     3241 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     3301 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     3361 agtatatatg tgtaacattg gtctagtgat tatttgccga ctaccttggt gatctcgcct
     3421 ttcacgtagt gaacaaattc ttccaactga tctgcgcgcg aggccaagcg atcttcttgt
     3481 ccaagataag cctgcctagc ttcaagtatg acgggctgat actgggccgg caggcgctcc
     3541 attgcccagt cggcagcgac atccttcggc gcgattttgc cggttactgc gctgtaccaa
     3601 atgcgggaca acgtaagcac tacatttcgc tcatcgccag cccagtcggg cggcgagttc
     3661 catagcgtta aggtttcatt tagcgcctca aatagatcct gttcaggaac cggatcaaag
     3721 agttcctccg ccgctggacc taccaaggca acgctatgtt ctcttgcttt tgtcagcaag
     3781 atagccagat caatgtcgat cgtggctggc tcgaagatac ctgcaagaat gtcattgcgc
     3841 tgccattctc caaattgcag ttcgcgctta gctggataac gccacggaat gatgtcgtcg
     3901 tgcacaacaa tggtgacttc tacagcgcgg agaatctcgc tctctccagg ggaagccgaa
     3961 gtttccaaaa ggtcgttgat caaagctcgc cgcgttgttt catcaagcct tacggtcacc
     4021 gtaaccagca aatcaatatc actgtgtggc ttcaggccgc catccactgc ggagccgtac
     4081 aaatgtacgg ccagcaacgt cggttcgaga tggcgctcga tgacgccaac tacctctgat
     4141 agttgagtcg atacttcggc gatcaccgct tccctcataa caccccttgt attactgttt
     4201 atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc aatgtaacat
     4261 cagagatttt gagacacaac gtggctttgt tgaataaatc gaacttttgc tgagttgaag
     4321 gatcagatca cgcatcttcc cgacaacgca gaccgttccg tggcaaagca aaagttcaaa
     4381 atcaccaact ggtccaccta caacaaagct ctcatcaacc gtggctccct cactttctgg
     4441 ctggatgatg gggcgattca ggcgatcccc atccaacagc ccgccgtcga gcgggct
//
