LOCUS       sequence-427362-       12861 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Controls for Bicistronic BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12861)
  AUTHORS   Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
  TITLE     Improved validation of protein interactions using bicistronic BiFC 
            (Bi2FC).
  JOURNAL   Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 
            10.1007/s12298-024-01477-y. Epub 2024 Jul 2.
  PUBMED    39100877
REFERENCE   2  (bases 1 to 12861)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12298-024-01477-y"; journalName: "Physiol Mol Biol 
            Plants"; date: "2024-07"; volume: "30"; issue: "7"; pages: 
            "1047-1054"
COMMENT     Sequence Label: pBi2FC-N-MPK5-P2A:mCherry
FEATURES             Location/Qualifiers
     source          1..12861
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1018..1038
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1163..1165,1166..1168,1169..1681)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1208..1229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1469..1488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1682..1711
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1730..1754
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2904..2928)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2936..2992
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2993..3013
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3014..3034
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3035..3055
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3056..3766
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3469..3488
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      3783..4035
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4068..4084)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4068..4084)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4081..4103)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4092..4108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4116..4122,4123..4140,4141..4146))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4161..4182
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4218..4470)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4621..5646)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5698..5877)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6383..6407
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6928..7719
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7965..8553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8454..8473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8707..8724
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8739..8879
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8805..8824)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8965..8987
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9223..9417
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9483..10556)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(10985..11614)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gaatggtgag caagggcgag gagctgttca ccggggtggt
     1201 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1261 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     1321 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     1381 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1441 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1501 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1561 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1621 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1681 ggagcaaaag ttgatttctg aggaggatct tgggcccagg cctactagta caagtttgta
     1741 caaaaaagca ggctccgcgg ccgcaatggc gaaggaaatt gaatcagcga cagatttagg
     1801 tgatactaac atcaaaggag ttcttgttca tggaggaaga tattttcaat acaatgttta
     1861 tggaaacttg tttgaagttt ctaataagta tgttcctcct attcgaccta ttggtagagg
     1921 agcttacggt tttgtctgtg ctgcagtaga ttcggagaca cacgaagaga ttgcgataaa
     1981 gaagataggt aaagcctttg ataataaagt agatgcaaag agaacattga gagagattaa
     2041 gctacttcgt catttagaac acgagaacgt tgttgttata aaagatatca ttagaccacc
     2101 aaagaaggaa gatttcgttg atgtttatat agtttttgag ttaatggata ctgatcttca
     2161 tcagattatt cgatcaaacc aatctttgaa tgatgatcat tgtcagtact tcttgtatca
     2221 gattctacga ggactcaaat atattcattc agcaaatgtt ttacatcgtg accttaagcc
     2281 gagtaatttg cttttgaatt cgaattgcga tcttaagatt actgactttg gtttggcaag
     2341 gactacatca gaaacagagt atatgacaga gtatgttgta acacgatggt acagagcacc
     2401 agagctgctt cttaatagtt ctgagtatac atctgctatt gatgtatggt ctgttggttg
     2461 catatttgct gagattatga caagggaacc gcttttcccg gggaaggatt acgttcatca
     2521 gcttaaactt atcactgagt tgataggatc acctgatgga gcgagtctag agtttctcag
     2581 aagcgcgaac gcgagaaaat atgttaaggg gctacctaag ttcccaagac aaaacttttc
     2641 agctaggttt cctagtatga attctacagc tatcgatcta ctagagaaaa tgctcgtgtt
     2701 tgatccagta aaacgtatca cggttgagga agcactgtgt tatccttact tgtcagctct
     2761 tcacgacctt aacgacgagc ctgtttgttc caaccatttc agtttccatt ttgaggatcc
     2821 gtcttccact gaagaagaga taaaggagct tgtgtggctc gaatctgtga agttcaatcc
     2881 tctgccgagc attaggcgcg ccgacccagc tttcttgtac aaagtggttc tcgaggcaac
     2941 caactttagc ctgctcaagc aagcaggaga tgttgaggaa aatcctggcc cccctaagaa
     3001 aaagagaaag gttccgaaaa agaaaagaaa ggtgccaaaa aaaaagagaa aggtaatggt
     3061 gagcaagggc gaggaggata acatggccat catcaaggag ttcatgcgct tcaaggtgca
     3121 catggagggc tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc
     3181 ctacgagggc acccagaccg ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc
     3241 ctgggacatc ctgtcccctc agttcatgta cggctccaag gcctacgtga agcaccccgc
     3301 cgacatcccc gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat
     3361 gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga
     3421 gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca
     3481 gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg acggcgccct
     3541 gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg acgctgaggt
     3601 caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca acgtcaacat
     3661 caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt acgaacgcgc
     3721 cgagggccgc cactccaccg gcggcatgga cgagctgtac aagtaagagc tcgaatttcc
     3781 ccgatcgttc aaacatttgg caataaagtt tcttaagatt gaatcctgtt gccggtcttg
     3841 cgatgattat catataattt ctgttgaatt acgttaagca tgtaataatt aacatgtaat
     3901 gcatgacgtt atttatgaga tgggttttta tgattagagt cccgcaatta tacatttaat
     3961 acgcgataga aaacaaaata tagcgcgcaa actaggataa attatcgcgc gcggtgtcat
     4021 ctatgttact agatcgggaa ttggttccgg aaccaattcg taatcatgtc atagctgttt
     4081 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     4141 tgtaaagcct ggggtgccta atgagtgagc taactcacat taggctgaat taggcgcgcc
     4201 tatttctatt aattcccgat ctagtaacat agatgacacc gcgcgcgata atttatccta
     4261 gtttgcgcgc tatattttgt tttctatcgc gtattaaatg tataattgcg ggactctaat
     4321 cataaaaacc catctcataa ataacgtcat gcattacatg ttaattatta catgcttaac
     4381 gtaattcaac agaaattata tgataatcat cgcaagaccg gcaacaggat tcaatcttaa
     4441 gaaactttat tgccaaatgt ttgaacgatc ggggaaattc gggggatcga tcccgcgaac
     4501 tgtggacgag aactgtgcca ccaagcgtaa ggccgttctc tcgcatttgc cttgctaggc
     4561 tcgcgcgagt tgctggctga ggcgttctcg aaatcagctc ttgttcggtc ggcatctact
     4621 ctattccttt gccctcggac gagtgctggg gcgtcggttt ccactatcgg cgagtacttc
     4681 tacacagcca tcggtccaga cggccgcgct tctgcgggcg atttgtgtac gcccgacagt
     4741 cccggctccg gatcggacga ttgcgtcgca tcgaccctgc gcccaagctg catcatcgaa
     4801 attgccgtca accaagctct gatagagttg gtcaagacca atgcggagca tatacgcccg
     4861 gagccgcggc gatcctgcaa gctccggatg cctccgctcg aagtagcgcg tctgctgctc
     4921 catacaagcc aaccacggcc tccagaagaa gatgttggcg acctcgtatt gggaatcccc
     4981 gaacatcgcc tcgctccagt caatgaccgc tgttatgcgg ccattgtccg tcaggacatt
     5041 gttggagccg aaatccgcgt gcacgaggtg ccggacttcg gggcagtcct cggcccaaag
     5101 catcagctca tcgagagcct gcgcgacgga cgcactgacg gtgtcgtcca tcacagtttg
     5161 ccagtgatac acatggggat cagcaatcgc gcatatgaaa tcacgccatg tagtgtattg
     5221 accgattcct tgcggtccga atgggccgaa cccgctcgtc tggctaagat cggccgcagc
     5281 gatcgcatcc atggcctccg cgaccggctg cagaacagcg ggcagttcgg tttcaggcag
     5341 gtcttgcaac gtgacaccct gtgcacggcg ggagatgcaa taggtcaggc tctcgctgaa
     5401 tgccccaatg tcaagcactt ccggaatcgg gagcgcggcc gatgcaaagt gccgataaac
     5461 ataacgatct ttgtagaaac catcggcgca gctatttacc cgcaggacat atccacgccc
     5521 tcctacatcg aagctgaaag cacgagattc ttcgccctcc gagagctgca tcaggtcgga
     5581 gacgctgtcg aacttttcga tcagaaactt ctcgacagac gtcgcggtga gttcaggctt
     5641 tttcatatct tattgccccc cgggatcaga tctggattga gagtgaatat gagactctaa
     5701 ttggataccg aggggaattt atggaacgtc agtggagcat ttttgacaag aaatatttgc
     5761 tagctgatag tgaccttagg cgacttttga acgcgcaata atggtttctg acgtatgtgc
     5821 ttagctcatt aaactccaga aacccgcggc tgagtggctc cttcaacgtt gcggttctgt
     5881 cagttccaaa cgtaaaacgg cttgtcccgc gtcatcggcg ggggtcataa cgtgactccc
     5941 ttaattctcc gctcatgatc ttgatcccct gcgccatcag atccttggcg gcaagaaagc
     6001 catccagttt actttgcagg gcttcccaac cttaccagag ggcgccccag ctggcaattc
     6061 cggttcgctt gctgtccata aaaccgccca gtctagctat cgccatgtaa gcccactgca
     6121 agctacctgc tttctctttg cgcttgcgtt ttcccttgtc cagatagccc agtagctgac
     6181 attcatccgg ggtcagcacc gtttctgcgg actggctttc tacgtgttcc gcttccttta
     6241 gcagcccttg cgccctgagt gcttgcggca gcgtgaagct gatccgtcga gattaataga
     6301 aataaattca gcctaattcg gcgttaattc agtacattaa aaacgtccgc aatgtgttat
     6361 taagttgtct aagcgtcaat ttgtttacac cacaatatat cctgccacca gccagccaac
     6421 agctccccga ccggcagctc ggcacaaaat caccactcga tacaggcagc ccatcagtcc
     6481 gggacggcgt cagcgggaga gccgttgtaa ggcggcagac tttgctcatg ttaccgatgc
     6541 tattcggaag aacggcaact aagctgccgg gtttgaaaca cggatgatct cgcggagggt
     6601 agcatgttga ttgtaacgat gacagagcgt tgctgcctgt gatcaattcg ggcacgaacc
     6661 cagtggacat aagcctgttc ggttcgtaag ctgtaatgca agtagcgtat gcgctcacgc
     6721 aactggtcca gaaccttgac cgaacgcagc ggtggtaacg gcgcagtggc ggttttcatg
     6781 gcttgttatg actgtttttt tggggtacag tctatgcctc gggcatccaa gcagcaagcg
     6841 cgttacgccg tgggtcgatg tttgatgtta tggagcagca acgatgttac gcagcagggc
     6901 agtcgcccta aaacaaagtt aaacatcatg ggggaagcgg tgatcgccga agtatcgact
     6961 caactatcag aggtagttgg cgtcatcgag cgccatctcg aaccgacgtt gctggccgta
     7021 catttgtacg gctccgcagt ggatggcggc ctgaagccac acagtgatat tgatttgctg
     7081 gttacggtga ccgtaaggct tgatgaaaca acgcggcgag ctttgatcaa cgaccttttg
     7141 gaaacttcgg cttcccctgg agagagcgag attctccgcg ctgtagaagt caccattgtt
     7201 gtgcacgacg acatcattcc gtggcgttat ccagctaagc gcgaactgca atttggagaa
     7261 tggcagcgca atgacattct tgcaggtatc ttcgagccag ccacgatcga cattgatctg
     7321 gctatcttgc tgacaaaagc aagagaacat agcgttgcct tggtaggtcc agcggcggag
     7381 gaactctttg atccggttcc tgaacaggat ctatttgagg cgctaaatga aaccttaacg
     7441 ctatggaact cgccgcccga ctgggctggc gatgagcgaa atgtagtgct tacgttgtcc
     7501 cgcatttggt acagcgcagt aaccggcaaa atcgcgccga aggatgtcgc tgccgactgg
     7561 gcaatggagc gcctgccggc ccagtatcag cccgtcatac ttgaagctag acaggcttat
     7621 cttggacaag aagaagatcg cttggcctcg cgcgcagatc agttggaaga atttgtccac
     7681 tacgtgaaag gcgagatcac caaggtagtc ggcaaataat gtctagctag aaattcgttc
     7741 aagccgacgc cgcttcgcgg cgcggcttaa ctcaagcgtt agatgcacta agcacataat
     7801 tgctcacagc caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa
     7861 gccctacaca aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg
     7921 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     7981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     8041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     8101 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     8161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     8221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     8281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     8341 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     8401 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     8461 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     8521 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     8581 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     8641 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     8701 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     8761 acgcatctgt gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat
     8821 gccgcatagt taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc
     8881 cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg
     8941 cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat
     9001 caccgaaacg cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc
     9061 gcggcttgtc cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca
     9121 gcggccgcga taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta
     9181 ggcgcttttt gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg
     9241 gttttaagag ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct
     9301 tttatatcag tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg
     9361 tacgggttcc ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga
     9421 aagagacctt ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta
     9481 cattaggaac cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc
     9541 gggccagcct gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc
     9601 agcttgcgca cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag
     9661 atcgtcttga acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag
     9721 agaaaacggc cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc
     9781 gggttcttgc cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac
     9841 tccggccgcc cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg
     9901 gataccgtca ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg
     9961 gtgtttaacc gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc
    10021 cggcagaact tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc
    10081 ttcccttccc ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg
    10141 tcgtaatccc acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct
    10201 ggaagctcgt agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg
    10261 gccacgtcca tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa
    10321 aaatctggtt gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc
    10381 ggttgccggg attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt
    10441 gcttctgcct cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg
    10501 tcatcaccca gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gctcacgccg
    10561 attcctcggg cttgggggtt ccagtgccat tgcagggccg gcagacaacc cagccgctta
    10621 cgcctggcca accgcccgtt cctccacaca tggggcattc cacggcgtcg gtgcctggtt
    10681 gttcttgatt ttccatgccg cctcctttag ccgctaaaat tcatctactc atttattcat
    10741 ttgctcattt actctggtag ctgcgcgatg tattcagata gcagctcggt aatggtcttg
    10801 ccttggcgta ccgcgtacat cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg
    10861 acccgcttca tggctggcgt gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt
    10921 gcgctcggac ggccggcact tagcgtgttt gtgcttttgc tcattttctc tttacctcat
    10981 taactcaaat gagttttgat ttaatttcag cggccagcgc ctggacctcg cgggcagcgt
    11041 cgccctcggg ttctgattca agaacggttg tgccggcggc ggcagtgcct gggtagctca
    11101 cgcgctgcgt gatacgggac tcaagaatgg gcagctcgta cccggccagc gcctcggcaa
    11161 cctcaccgcc gatgcgcgtg cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc
    11221 ttccatccgt gacctcaatg cgctgcttaa ccagctccac caggtcggcg gtggcccata
    11281 tgtcgtaagg gcttggctgc accggaatca gcacgaagtc ggctgccttg atcgcggaca
    11341 cagccaagtc cgccgcctgg ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg
    11401 ccttcacgtc gcggtcaatc gtcgggcggt cgatgccgac aacggttagc ggttgatctt
    11461 cccgcacggc cgcccaatcg cgggcactgc cctggggatc ggaatcgact aacagaacat
    11521 cggccccggc gagttgcagg gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc
    11581 cgcctttctg gttaagtaca gcgataacct tcatgcgttc cccttgcgta tttgtttatt
    11641 tactcatcgc atcatatacg cagcgaccgc atgacgcaag ctgttttact caaatacaca
    11701 tcaccttttt agacggcggc gctcggtttc ttcagcggcc aagctggccg gccaggccgc
    11761 cagcttggca tcagacaaac cggccaggat ttcatgcagc cgcacggttg agacgtgcgc
    11821 gggcggctcg aacacgtacc cggccgcgat catctccgcc tcgatctctt cggtaatgaa
    11881 aaacggttcg tcctggccgt cctggtgcgg tttcatgctt gttcctcttg gcgttcattc
    11941 tcggcggccg ccagggcgtc ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc
    12001 ctggcctcgg tgggcgtcac ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga
    12061 tgcacgccaa gcagtgcagc cgcctctttc acggtgcggc cttcctggtc gatcagctcg
    12121 cgggcgtgcg cgatctgtgc cggggtgagg gtagggcggg ggccaaactt cacgcctcgg
    12181 gccttggcgg cctcgcgccc gctccgggtg cggtcgatga ttagggaacg ctcgaactcg
    12241 gcaatgccgg cgaacacggt caacaccatg cggccggccg gcgtggtggt gtcggcccac
    12301 ggctctgcca ggctacgcag gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt
    12361 aggtcgcggg tgctgcgggc caggcggtct agcctggtca ctgtcacaac gtcgccaggg
    12421 cgtaggtggt caagcatcct ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc
    12481 tcggaaaaca gcttggtgca gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc
    12541 tggtcgtcgg tgctgacgcg ggcatagccc agcaggccag cggcggcgct cttgttcatg
    12601 gcgtaatgtc tccggttcta gtcgcaagta ttctacttta tgcgactaaa acacgcgaca
    12661 agaaaacgcc aggaaaaggg cagggcggca gcctgtcgcg taacttagga cttgtgcgac
    12721 atgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact atagcagcgg
    12781 aggggttgga tcaaagtact ttgatcccga ggggaaccct gtggttggca tgcacataca
    12841 aatggacgaa cggataaacc t
//
