LOCUS       sequence-427363-       12951 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Controls for Bicistronic BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12951)
  AUTHORS   Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
  TITLE     Improved validation of protein interactions using bicistronic BiFC 
            (Bi2FC).
  JOURNAL   Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 
            10.1007/s12298-024-01477-y. Epub 2024 Jul 2.
  PUBMED    39100877
REFERENCE   2  (bases 1 to 12951)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12298-024-01477-y"; journalName: "Physiol Mol Biol 
            Plants"; date: "2024-07"; volume: "30"; issue: "7"; pages: 
            "1047-1054"
COMMENT     Sequence Label: pBi2FC-N-MPK12-P2A:mCherry
FEATURES             Location/Qualifiers
     source          1..12951
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1018..1038
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1163..1165,1166..1168,1169..1681)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1208..1229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1469..1488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1682..1711
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1730..1754
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2994..3018)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3026..3082
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3083..3103
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3104..3124
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3125..3145
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3146..3856
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     3559..3578
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      3873..4125
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4158..4174)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4158..4174)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4171..4193)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4182..4198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4206..4212,4213..4230,4231..4236))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4251..4272
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4308..4560)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4711..5736)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(5788..5967)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    6473..6497
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7018..7809
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8055..8643
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8544..8563
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8797..8814
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    8829..8969
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(8895..8914)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9055..9077
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9313..9507
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(9573..10646)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11075..11704)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gaatggtgag caagggcgag gagctgttca ccggggtggt
     1201 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1261 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     1321 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     1381 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1441 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1501 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1561 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1621 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1681 ggagcaaaag ttgatttctg aggaggatct tgggcccagg cctactagta caagtttgta
     1741 caaaaaagca ggctccgcgg ccgcaatgga tttagtgtct tcaagagata ctttaggaga
     1801 tccttcaact tcaacaaaag cccttcttct tggtttctct cgttctcggt ctgatcattg
     1861 catcatcatg tctggagaat caagctctgg ttctaccgaa cattgcatca aagttgtacc
     1921 gacacacggt ggccgctatg ttcagtacaa cgtttatgga caactctttg aagtttccag
     1981 aaagtatgtc cctcctattc gtcccattgg tagaggcgct tgcggtattg tctgtgctgc
     2041 ggtgaactca gtgactggag agaaagtggc tattaagaag atcggtaatg cttttgataa
     2101 catcatcgat gctaagagaa cgctacgtga aattaaactt ctcaggcata tggatcatga
     2161 gaacgttata accatcaaag atattgtaag acctccgcaa cgagatatct tcaatgatgt
     2221 ctacattgtc tatgagttaa tggacactga tcttcagcga atcctccgtt ctaaccaaac
     2281 actgaccagt gatcaatgcc gtttcctagt ataccagctc ttaagagggc tcaaatacgt
     2341 gcactcggcc aacatattac atcgtgatct aaggccaagc aacgtgctac ttaactcgaa
     2401 aaacgagcta aagattggtg attttgggct tgcaagaaca acttccgaca cagacttcat
     2461 gactgaatat gtcgttaccc gttggtacag ggctcctgag ttgcttctta actgctcaga
     2521 gtacaccgca gctattgata tttggtctgt tggctgcata ctcggcgaaa tcatgacggg
     2581 acaaccgttg tttccaggca aagattatgt tcatcagctt aggcttataa cagagcttgt
     2641 aggctctcca gacaattcca gcctcggctt ccttcgcagt gacaacgcaa gaagatacgt
     2701 caggcaactt ccgcgatacc cgaaacaaca gtttgctgct agattcccga aaatgcccac
     2761 tacggctatc gatttgcttg agagaatgct cgtctttgat cctaaccggc gcatctcagt
     2821 cgatgaagcc cttggtcatg cttacctatc accgcaccat gatgtggcca aagaaccggt
     2881 ctgttcgact cctttcagct ttgatttcga acatccttct tgcacagaag aacacataaa
     2941 ggagcttatc tacaaggagt ctgtcaaatt caatcctgac cacaggcgcg ccgacccagc
     3001 tttcttgtac aaagtggttc tcgaggcaac caactttagc ctgctcaagc aagcaggaga
     3061 tgttgaggaa aatcctggcc cccctaagaa aaagagaaag gttccgaaaa agaaaagaaa
     3121 ggtgccaaaa aaaaagagaa aggtaatggt gagcaagggc gaggaggata acatggccat
     3181 catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt
     3241 cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa
     3301 ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta
     3361 cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt
     3421 ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt
     3481 gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac
     3541 caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc
     3601 cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct
     3661 gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt
     3721 gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga
     3781 ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga
     3841 cgagctgtac aagtaagagc tcgaatttcc ccgatcgttc aaacatttgg caataaagtt
     3901 tcttaagatt gaatcctgtt gccggtcttg cgatgattat catataattt ctgttgaatt
     3961 acgttaagca tgtaataatt aacatgtaat gcatgacgtt atttatgaga tgggttttta
     4021 tgattagagt cccgcaatta tacatttaat acgcgataga aaacaaaata tagcgcgcaa
     4081 actaggataa attatcgcgc gcggtgtcat ctatgttact agatcgggaa ttggttccgg
     4141 aaccaattcg taatcatgtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     4201 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     4261 taactcacat taggctgaat taggcgcgcc tatttctatt aattcccgat ctagtaacat
     4321 agatgacacc gcgcgcgata atttatccta gtttgcgcgc tatattttgt tttctatcgc
     4381 gtattaaatg tataattgcg ggactctaat cataaaaacc catctcataa ataacgtcat
     4441 gcattacatg ttaattatta catgcttaac gtaattcaac agaaattata tgataatcat
     4501 cgcaagaccg gcaacaggat tcaatcttaa gaaactttat tgccaaatgt ttgaacgatc
     4561 ggggaaattc gggggatcga tcccgcgaac tgtggacgag aactgtgcca ccaagcgtaa
     4621 ggccgttctc tcgcatttgc cttgctaggc tcgcgcgagt tgctggctga ggcgttctcg
     4681 aaatcagctc ttgttcggtc ggcatctact ctattccttt gccctcggac gagtgctggg
     4741 gcgtcggttt ccactatcgg cgagtacttc tacacagcca tcggtccaga cggccgcgct
     4801 tctgcgggcg atttgtgtac gcccgacagt cccggctccg gatcggacga ttgcgtcgca
     4861 tcgaccctgc gcccaagctg catcatcgaa attgccgtca accaagctct gatagagttg
     4921 gtcaagacca atgcggagca tatacgcccg gagccgcggc gatcctgcaa gctccggatg
     4981 cctccgctcg aagtagcgcg tctgctgctc catacaagcc aaccacggcc tccagaagaa
     5041 gatgttggcg acctcgtatt gggaatcccc gaacatcgcc tcgctccagt caatgaccgc
     5101 tgttatgcgg ccattgtccg tcaggacatt gttggagccg aaatccgcgt gcacgaggtg
     5161 ccggacttcg gggcagtcct cggcccaaag catcagctca tcgagagcct gcgcgacgga
     5221 cgcactgacg gtgtcgtcca tcacagtttg ccagtgatac acatggggat cagcaatcgc
     5281 gcatatgaaa tcacgccatg tagtgtattg accgattcct tgcggtccga atgggccgaa
     5341 cccgctcgtc tggctaagat cggccgcagc gatcgcatcc atggcctccg cgaccggctg
     5401 cagaacagcg ggcagttcgg tttcaggcag gtcttgcaac gtgacaccct gtgcacggcg
     5461 ggagatgcaa taggtcaggc tctcgctgaa tgccccaatg tcaagcactt ccggaatcgg
     5521 gagcgcggcc gatgcaaagt gccgataaac ataacgatct ttgtagaaac catcggcgca
     5581 gctatttacc cgcaggacat atccacgccc tcctacatcg aagctgaaag cacgagattc
     5641 ttcgccctcc gagagctgca tcaggtcgga gacgctgtcg aacttttcga tcagaaactt
     5701 ctcgacagac gtcgcggtga gttcaggctt tttcatatct tattgccccc cgggatcaga
     5761 tctggattga gagtgaatat gagactctaa ttggataccg aggggaattt atggaacgtc
     5821 agtggagcat ttttgacaag aaatatttgc tagctgatag tgaccttagg cgacttttga
     5881 acgcgcaata atggtttctg acgtatgtgc ttagctcatt aaactccaga aacccgcggc
     5941 tgagtggctc cttcaacgtt gcggttctgt cagttccaaa cgtaaaacgg cttgtcccgc
     6001 gtcatcggcg ggggtcataa cgtgactccc ttaattctcc gctcatgatc ttgatcccct
     6061 gcgccatcag atccttggcg gcaagaaagc catccagttt actttgcagg gcttcccaac
     6121 cttaccagag ggcgccccag ctggcaattc cggttcgctt gctgtccata aaaccgccca
     6181 gtctagctat cgccatgtaa gcccactgca agctacctgc tttctctttg cgcttgcgtt
     6241 ttcccttgtc cagatagccc agtagctgac attcatccgg ggtcagcacc gtttctgcgg
     6301 actggctttc tacgtgttcc gcttccttta gcagcccttg cgccctgagt gcttgcggca
     6361 gcgtgaagct gatccgtcga gattaataga aataaattca gcctaattcg gcgttaattc
     6421 agtacattaa aaacgtccgc aatgtgttat taagttgtct aagcgtcaat ttgtttacac
     6481 cacaatatat cctgccacca gccagccaac agctccccga ccggcagctc ggcacaaaat
     6541 caccactcga tacaggcagc ccatcagtcc gggacggcgt cagcgggaga gccgttgtaa
     6601 ggcggcagac tttgctcatg ttaccgatgc tattcggaag aacggcaact aagctgccgg
     6661 gtttgaaaca cggatgatct cgcggagggt agcatgttga ttgtaacgat gacagagcgt
     6721 tgctgcctgt gatcaattcg ggcacgaacc cagtggacat aagcctgttc ggttcgtaag
     6781 ctgtaatgca agtagcgtat gcgctcacgc aactggtcca gaaccttgac cgaacgcagc
     6841 ggtggtaacg gcgcagtggc ggttttcatg gcttgttatg actgtttttt tggggtacag
     6901 tctatgcctc gggcatccaa gcagcaagcg cgttacgccg tgggtcgatg tttgatgtta
     6961 tggagcagca acgatgttac gcagcagggc agtcgcccta aaacaaagtt aaacatcatg
     7021 ggggaagcgg tgatcgccga agtatcgact caactatcag aggtagttgg cgtcatcgag
     7081 cgccatctcg aaccgacgtt gctggccgta catttgtacg gctccgcagt ggatggcggc
     7141 ctgaagccac acagtgatat tgatttgctg gttacggtga ccgtaaggct tgatgaaaca
     7201 acgcggcgag ctttgatcaa cgaccttttg gaaacttcgg cttcccctgg agagagcgag
     7261 attctccgcg ctgtagaagt caccattgtt gtgcacgacg acatcattcc gtggcgttat
     7321 ccagctaagc gcgaactgca atttggagaa tggcagcgca atgacattct tgcaggtatc
     7381 ttcgagccag ccacgatcga cattgatctg gctatcttgc tgacaaaagc aagagaacat
     7441 agcgttgcct tggtaggtcc agcggcggag gaactctttg atccggttcc tgaacaggat
     7501 ctatttgagg cgctaaatga aaccttaacg ctatggaact cgccgcccga ctgggctggc
     7561 gatgagcgaa atgtagtgct tacgttgtcc cgcatttggt acagcgcagt aaccggcaaa
     7621 atcgcgccga aggatgtcgc tgccgactgg gcaatggagc gcctgccggc ccagtatcag
     7681 cccgtcatac ttgaagctag acaggcttat cttggacaag aagaagatcg cttggcctcg
     7741 cgcgcagatc agttggaaga atttgtccac tacgtgaaag gcgagatcac caaggtagtc
     7801 ggcaaataat gtctagctag aaattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     7861 ctcaagcgtt agatgcacta agcacataat tgctcacagc caaactatca ggtcaagtct
     7921 gcttttatta tttttaagcg tgcataataa gccctacaca aattgggaga tatatcatgc
     7981 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8041 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     8101 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     8161 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
     8221 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     8281 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     8341 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     8401 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     8461 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     8521 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     8581 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     8641 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8701 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
     8761 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
     8821 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata
     8881 tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc
     8941 tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc
     9001 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     9061 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ggtgccttga
     9121 tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg tagattgcct
     9181 ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg cgaagcggcg
     9241 gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttc ggctgtgcgc
     9301 tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag ttttaaagag
     9361 ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat gtgtgaccgg
     9421 ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat gtacggcttt
     9481 gggttcccaa tgtacgtgct atccacagga aagagacctt ttcgaccttt ttcccctgct
     9541 agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc ttcgccctcg
     9601 atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc ctccttcaaa
     9661 tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca gaacttcttg
     9721 aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct ggcttctgcc
     9781 ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg atcgatcaaa
     9841 aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat ctcgcggtac
     9901 atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct ctttacgatc
     9961 ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc gttcttggcc
    10021 ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt ttctaccagg
    10081 tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc cgcaacgtgt
    10141 ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt catggattcg
    10201 gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc catgccggcc
    10261 ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac ctcgccagct
    10321 cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg actatcgcgg
    10381 gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc cttgggcggc
    10441 ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg gattcgatca
    10501 gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg ccgctgggcg
    10561 gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc gatttgtacc
    10621 gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt ccagtgccat
    10681 tgcagggccg gcagacaacc cagccgctta cgcctggcca accgcccgtt cctccacaca
    10741 tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg cctcctttag
    10801 ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag ctgcgcgatg
    10861 tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat cttcagcttg
    10921 gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt gtctgccagg
    10981 ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact tagcgtgttt
    11041 gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat ttaatttcag
    11101 cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca agaacggttg
    11161 tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac tcaagaatgg
    11221 gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg cctttgatcg
    11281 cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg cgctgcttaa
    11341 ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc accggaatca
    11401 gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg ggcgctccgt
    11461 cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc gtcgggcggt
    11521 cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg cgggcactgc
    11581 cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg gcgcgggcta
    11641 gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca gcgataacct
    11701 tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg cagcgaccgc
    11761 atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc gctcggtttc
    11821 ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac cggccaggat
    11881 ttcatgcagc cgcacggttg agacgtgcgc gggcggctcg aacacgtacc cggccgcgat
    11941 catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt cctggtgcgg
    12001 tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc ggcctcggtc
    12061 aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac ttcctcgctg
    12121 cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc cgcctctttc
    12181 acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc cggggtgagg
    12241 gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc gctccgggtg
    12301 cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt caacaccatg
    12361 cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag gcccgcgccg
    12421 gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc caggcggtct
    12481 agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct ggccagctcc
    12541 gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca gccggccgcg
    12601 tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg ggcatagccc
    12661 agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta gtcgcaagta
    12721 ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg cagggcggca
    12781 gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg ctgcactgaa
    12841 cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact ttgatcccga
    12901 ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc t
//
