LOCUS       sequence-427364-       13500 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Controls for Bicistronic BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13500)
  AUTHORS   Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
  TITLE     Improved validation of protein interactions using bicistronic BiFC 
            (Bi2FC).
  JOURNAL   Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 
            10.1007/s12298-024-01477-y. Epub 2024 Jul 2.
  PUBMED    39100877
REFERENCE   2  (bases 1 to 13500)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12298-024-01477-y"; journalName: "Physiol Mol Biol 
            Plants"; date: "2024-07"; volume: "30"; issue: "7"; pages: 
            "1047-1054"
COMMENT     Sequence Label: pBi2FC-N-MPK8-P2A:mCherry
FEATURES             Location/Qualifiers
     source          1..13500
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1018..1038
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             join(1163..1165,1166..1168,1169..1681)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KLICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIK
                     ANFKIRHNIE"
     primer_bind     complement(1208..1229)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1469..1488)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             1682..1711
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    1730..1754
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(3543..3567)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             3575..3631
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             3632..3652
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3653..3673
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3674..3694
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3695..4405
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     4108..4127
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     terminator      4422..4674
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(4707..4723)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4707..4723)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4720..4742)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4731..4747
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4755..4761,4762..4779,4780..4785))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4800..4821
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(4857..5109)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(5260..6285)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(6337..6516)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    7022..7046
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7567..8358
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      8604..9192
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9093..9112
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9346..9363
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9378..9518
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(9444..9463)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9604..9626
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      9862..10056
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10122..11195)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(11624..12253)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gaatggtgag caagggcgag gagctgttca ccggggtggt
     1201 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     1261 gggcgagggc gatgccacct acggcaagct gaccctgaag ctgatctgca ccaccggcaa
     1321 gctgcccgtg ccctggccca ccctcgtgac caccctgggc tacggcctgc agtgcttcgc
     1381 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     1441 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     1501 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     1561 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     1621 caccgccgac aagcagaaga acggcatcaa ggccaacttc aagatccgcc acaacatcga
     1681 ggagcaaaag ttgatttctg aggaggatct tgggcccagg cctactagta caagtttgta
     1741 caaaaaagca ggctccgcgg ccgcaatggg tggtggtggg aatctcgtcg acggtgttcg
     1801 tcgatggctt tttcagcgac cttcttcttc ttcttcttct tcttcttcca acaataataa
     1861 taataatcac gaacaaccca ttttcaattc ttcttctttc tcttcttcct ctaaccccaa
     1921 tcattccgct aactctggag agcttattat tgaagaagat ctcgatttct ctggtctaac
     1981 gcttattaac gttcctaaac gtaatcattt acctatggat cctcacaaga agggtgaaac
     2041 tgaattcttc acggagtatg gagaagctaa caggtatcag attcaagagg ttgttggcaa
     2101 aggaagttat ggtgttgtgg cttctgctgt tgactcacac actggagaga gagttgcgat
     2161 caagaagatc aacgatgtgt ttgagcatgt ctccgatgct actaggattc tcagagagat
     2221 caaattgctt cggttgcttc gtcatcctga tgttgtggag attaaacaca tcatgttacc
     2281 tccttctcgc agagaattcc gcgatattta tgttgtcttt gagttgatgg aatctgatct
     2341 tcatcaagtg atcaaggcga atgatgattt gactcctgag cattatcagt ttttcttgta
     2401 ccagcttctt cgtggtctga aatatgttca cgcagctaat gtgtttcatc gggatttgaa
     2461 accgaagaac attctagcta atgctgattg caaattgaag atctgtgatt ttgggctggc
     2521 tcgcgtttct tttaacgatg ccccaaccgc tatattttgg actgattatg tagctactcg
     2581 gtggtatcgt gcccctgaac tctgtggatc gtttttctcc aaatataccc ctgcgattga
     2641 tatctggagt gtcggttgca tttttgcgga aatgcttttg ggtaagcctt tgtttcccgg
     2701 gaaaaacgtt gtgcaccaat tggatctaat gactgacttt cttggtactc cacctcctga
     2761 gtcaatatca aggattagaa atgaaaaggc gaggagatat cttagcagca tgaggaaaaa
     2821 acaaccagtc cctttctctc ataaattccc taaagctgat cctttggctc tccgccttct
     2881 ggaacgcctg cttgcctttg atccgaaaga tcgtgcatca gctgaagatg cactagctga
     2941 tccatatttc agtggtttgt caaattcaga gcgtgaacca acaacacagc caatttcaaa
     3001 gcttgaattt gactttgaga gaaagaagtt agttaaagat gatgtcagag aattaattta
     3061 ccgagagata ttggaatatc atcctcagat gttggaggaa taccttcgtg gtggtgatca
     3121 gcttagcttt atgtacccaa gtggggtaga ccgatttaaa aggcaatttg ctcatcttga
     3181 agaaaatcaa ggtaaaccag gagcagcagg aggaggaaga agtactgcac ttcacagaca
     3241 tcatgcttcc ttgccaagag agagagttcc tgctccaaat ggtgaaactg cagaagaaag
     3301 cagtgatgtt gagagaagag cagcagctgc tgtggcttca accttggaat ccgaggaagc
     3361 agacaatgga ggaggttaca gtgctcgtaa cctcatgaag agtgcaagca tcagcggttc
     3421 taaatgcatt ggtgtccaat ctaaaactga caaagaggat accatagctg aggaagaaga
     3481 taatgaaaca gttgcagagc ttactgataa agttgcttct cttcacaatt ctaggcgcgc
     3541 cgacccagct ttcttgtaca aagtggttct cgaggcaacc aactttagcc tgctcaagca
     3601 agcaggagat gttgaggaaa atcctggccc ccctaagaaa aagagaaagg ttccgaaaaa
     3661 gaaaagaaag gtgccaaaaa aaaagagaaa ggtaatggtg agcaagggcg aggaggataa
     3721 catggccatc atcaaggagt tcatgcgctt caaggtgcac atggagggct ccgtgaacgg
     3781 ccacgagttc gagatcgagg gcgagggcga gggccgcccc tacgagggca cccagaccgc
     3841 caagctgaag gtgaccaagg gtggccccct gcccttcgcc tgggacatcc tgtcccctca
     3901 gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc gacatccccg actacttgaa
     3961 gctgtccttc cccgagggct tcaagtggga gcgcgtgatg aacttcgagg acggcggcgt
     4021 ggtgaccgtg acccaggact cctccctgca ggacggcgag ttcatctaca aggtgaagct
     4081 gcgcggcacc aacttcccct ccgacggccc cgtaatgcag aagaagacca tgggctggga
     4141 ggcctcctcc gagcggatgt accccgagga cggcgccctg aagggcgaga tcaagcagag
     4201 gctgaagctg aaggacggcg gccactacga cgctgaggtc aagaccacct acaaggccaa
     4261 gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc aagttggaca tcacctccca
     4321 caacgaggac tacaccatcg tggaacagta cgaacgcgcc gagggccgcc actccaccgg
     4381 cggcatggac gagctgtaca agtaagagct cgaatttccc cgatcgttca aacatttggc
     4441 aataaagttt cttaagattg aatcctgttg ccggtcttgc gatgattatc atataatttc
     4501 tgttgaatta cgttaagcat gtaataatta acatgtaatg catgacgtta tttatgagat
     4561 gggtttttat gattagagtc ccgcaattat acatttaata cgcgatagaa aacaaaatat
     4621 agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc tatgttacta gatcgggaat
     4681 tggttccgga accaattcgt aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg
     4741 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     4801 tgagtgagct aactcacatt aggctgaatt aggcgcgcct atttctatta attcccgatc
     4861 tagtaacata gatgacaccg cgcgcgataa tttatcctag tttgcgcgct atattttgtt
     4921 ttctatcgcg tattaaatgt ataattgcgg gactctaatc ataaaaaccc atctcataaa
     4981 taacgtcatg cattacatgt taattattac atgcttaacg taattcaaca gaaattatat
     5041 gataatcatc gcaagaccgg caacaggatt caatcttaag aaactttatt gccaaatgtt
     5101 tgaacgatcg gggaaattcg ggggatcgat cccgcgaact gtggacgaga actgtgccac
     5161 caagcgtaag gccgttctct cgcatttgcc ttgctaggct cgcgcgagtt gctggctgag
     5221 gcgttctcga aatcagctct tgttcggtcg gcatctactc tattcctttg ccctcggacg
     5281 agtgctgggg cgtcggtttc cactatcggc gagtacttct acacagccat cggtccagac
     5341 ggccgcgctt ctgcgggcga tttgtgtacg cccgacagtc ccggctccgg atcggacgat
     5401 tgcgtcgcat cgaccctgcg cccaagctgc atcatcgaaa ttgccgtcaa ccaagctctg
     5461 atagagttgg tcaagaccaa tgcggagcat atacgcccgg agccgcggcg atcctgcaag
     5521 ctccggatgc ctccgctcga agtagcgcgt ctgctgctcc atacaagcca accacggcct
     5581 ccagaagaag atgttggcga cctcgtattg ggaatccccg aacatcgcct cgctccagtc
     5641 aatgaccgct gttatgcggc cattgtccgt caggacattg ttggagccga aatccgcgtg
     5701 cacgaggtgc cggacttcgg ggcagtcctc ggcccaaagc atcagctcat cgagagcctg
     5761 cgcgacggac gcactgacgg tgtcgtccat cacagtttgc cagtgataca catggggatc
     5821 agcaatcgcg catatgaaat cacgccatgt agtgtattga ccgattcctt gcggtccgaa
     5881 tgggccgaac ccgctcgtct ggctaagatc ggccgcagcg atcgcatcca tggcctccgc
     5941 gaccggctgc agaacagcgg gcagttcggt ttcaggcagg tcttgcaacg tgacaccctg
     6001 tgcacggcgg gagatgcaat aggtcaggct ctcgctgaat gccccaatgt caagcacttc
     6061 cggaatcggg agcgcggccg atgcaaagtg ccgataaaca taacgatctt tgtagaaacc
     6121 atcggcgcag ctatttaccc gcaggacata tccacgccct cctacatcga agctgaaagc
     6181 acgagattct tcgccctccg agagctgcat caggtcggag acgctgtcga acttttcgat
     6241 cagaaacttc tcgacagacg tcgcggtgag ttcaggcttt ttcatatctt attgcccccc
     6301 gggatcagat ctggattgag agtgaatatg agactctaat tggataccga ggggaattta
     6361 tggaacgtca gtggagcatt tttgacaaga aatatttgct agctgatagt gaccttaggc
     6421 gacttttgaa cgcgcaataa tggtttctga cgtatgtgct tagctcatta aactccagaa
     6481 acccgcggct gagtggctcc ttcaacgttg cggttctgtc agttccaaac gtaaaacggc
     6541 ttgtcccgcg tcatcggcgg gggtcataac gtgactccct taattctccg ctcatgatct
     6601 tgatcccctg cgccatcaga tccttggcgg caagaaagcc atccagttta ctttgcaggg
     6661 cttcccaacc ttaccagagg gcgccccagc tggcaattcc ggttcgcttg ctgtccataa
     6721 aaccgcccag tctagctatc gccatgtaag cccactgcaa gctacctgct ttctctttgc
     6781 gcttgcgttt tcccttgtcc agatagccca gtagctgaca ttcatccggg gtcagcaccg
     6841 tttctgcgga ctggctttct acgtgttccg cttcctttag cagcccttgc gccctgagtg
     6901 cttgcggcag cgtgaagctg atccgtcgag attaatagaa ataaattcag cctaattcgg
     6961 cgttaattca gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt
     7021 tgtttacacc acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg
     7081 gcacaaaatc accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag
     7141 ccgttgtaag gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta
     7201 agctgccggg tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg
     7261 acagagcgtt gctgcctgtg atcaattcgg gcacgaaccc agtggacata agcctgttcg
     7321 gttcgtaagc tgtaatgcaa gtagcgtatg cgctcacgca actggtccag aaccttgacc
     7381 gaacgcagcg gtggtaacgg cgcagtggcg gttttcatgg cttgttatga ctgttttttt
     7441 ggggtacagt ctatgcctcg ggcatccaag cagcaagcgc gttacgccgt gggtcgatgt
     7501 ttgatgttat ggagcagcaa cgatgttacg cagcagggca gtcgccctaa aacaaagtta
     7561 aacatcatgg gggaagcggt gatcgccgaa gtatcgactc aactatcaga ggtagttggc
     7621 gtcatcgagc gccatctcga accgacgttg ctggccgtac atttgtacgg ctccgcagtg
     7681 gatggcggcc tgaagccaca cagtgatatt gatttgctgg ttacggtgac cgtaaggctt
     7741 gatgaaacaa cgcggcgagc tttgatcaac gaccttttgg aaacttcggc ttcccctgga
     7801 gagagcgaga ttctccgcgc tgtagaagtc accattgttg tgcacgacga catcattccg
     7861 tggcgttatc cagctaagcg cgaactgcaa tttggagaat ggcagcgcaa tgacattctt
     7921 gcaggtatct tcgagccagc cacgatcgac attgatctgg ctatcttgct gacaaaagca
     7981 agagaacata gcgttgcctt ggtaggtcca gcggcggagg aactctttga tccggttcct
     8041 gaacaggatc tatttgaggc gctaaatgaa accttaacgc tatggaactc gccgcccgac
     8101 tgggctggcg atgagcgaaa tgtagtgctt acgttgtccc gcatttggta cagcgcagta
     8161 accggcaaaa tcgcgccgaa ggatgtcgct gccgactggg caatggagcg cctgccggcc
     8221 cagtatcagc ccgtcatact tgaagctaga caggcttatc ttggacaaga agaagatcgc
     8281 ttggcctcgc gcgcagatca gttggaagaa tttgtccact acgtgaaagg cgagatcacc
     8341 aaggtagtcg gcaaataatg tctagctaga aattcgttca agccgacgcc gcttcgcggc
     8401 gcggcttaac tcaagcgtta gatgcactaa gcacataatt gctcacagcc aaactatcag
     8461 gtcaagtctg cttttattat ttttaagcgt gcataataag ccctacacaa attgggagat
     8521 atatcatgca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8581 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8641 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8701 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     8761 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8821 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8881 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8941 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     9001 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     9061 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     9121 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     9181 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     9241 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     9301 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     9361 gaggaagcgg aagagcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca
     9421 caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat
     9481 acactccgct atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg
     9541 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     9601 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagg
     9661 gtgccttgat gtgggcgccg gcggtcgagt ggcgacggcg cggcttgtcc gcgccctggt
     9721 agattgcctg gccgtaggcc agccattttt gagcggccag cggccgcgat aggccgacgc
     9781 gaagcggcgg ggcgtaggga gcgcagcgac cgaagggtag gcgctttttg cagctcttcg
     9841 gctgtgcgct ggccagacag ttatgcacag gccaggcggg ttttaagagt tttaataagt
     9901 tttaaagagt tttaggcgga aaaatcgcct tttttctctt ttatatcagt cacttacatg
     9961 tgtgaccggt tcccaatgta cggctttggg ttcccaatgt acgggttccg gttcccaatg
    10021 tacggctttg ggttcccaat gtacgtgcta tccacaggaa agagaccttt tcgacctttt
    10081 tcccctgcta gggcaatttg ccctagcatc tgctccgtac attaggaacc ggcggatgct
    10141 tcgccctcga tcaggttgcg gtagcgcatg actaggatcg ggccagcctg ccccgcctcc
    10201 tccttcaaat cgtactccgg caggtcattt gacccgatca gcttgcgcac ggtgaaacag
    10261 aacttcttga actctccggc gctgccactg cgttcgtaga tcgtcttgaa caaccatctg
    10321 gcttctgcct tgcctgcggc gcggcgtgcc aggcggtaga gaaaacggcc gatgccggga
    10381 tcgatcaaaa agtaatcggg gtgaaccgtc agcacgtccg ggttcttgcc ttctgtgatc
    10441 tcgcggtaca tccaatcagc tagctcgatc tcgatgtact ccggccgccc ggtttcgctc
    10501 tttacgatct tgtagcggct aatcaaggct tcaccctcgg ataccgtcac caggcggccg
    10561 ttcttggcct tcttcgtacg ctgcatggca acgtgcgtgg tgtttaaccg aatgcaggtt
    10621 tctaccaggt cgtctttctg ctttccgcca tcggctcgcc ggcagaactt gagtacgtcc
    10681 gcaacgtgtg gacggaacac gcggccgggc ttgtctccct tcccttcccg gtatcggttc
    10741 atggattcgg ttagatggga aaccgccatc agtaccaggt cgtaatccca cacactggcc
    10801 atgccggccg gccctgcgga aacctctacg tgcccgtctg gaagctcgta gcggatcacc
    10861 tcgccagctc gtcggtcacg cttcgacaga cggaaaacgg ccacgtccat gatgctgcga
    10921 ctatcgcggg tgcccacgtc atagagcatc ggaacgaaaa aatctggttg ctcgtcgccc
    10981 ttgggcggct tcctaatcga cggcgcaccg gctgccggcg gttgccggga ttctttgcgg
    11041 attcgatcag cggccgcttg ccacgattca ccggggcgtg cttctgcctc gatgcgttgc
    11101 cgctgggcgg cctgcgcggc cttcaacttc tccaccaggt catcacccag cgccgcgccg
    11161 atttgtaccg ggccggatgg tttgcgaccg ctcacgccga ttcctcgggc ttgggggttc
    11221 cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa ccgcccgttc
    11281 ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt tccatgccgc
    11341 ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta ctctggtagc
    11401 tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac cgcgtacatc
    11461 ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat ggctggcgtg
    11521 tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg gccggcactt
    11581 agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg agttttgatt
    11641 taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt tctgattcaa
    11701 gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg atacgggact
    11761 caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg atgcgcgtgc
    11821 ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg acctcaatgc
    11881 gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg cttggctgca
    11941 ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc gccgcctggg
    12001 gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg cggtcaatcg
    12061 tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc gcccaatcgc
    12121 gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg agttgcaggg
    12181 cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg ttaagtacag
    12241 cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca tcatatacgc
    12301 agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta gacggcggcg
    12361 ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat cagacaaacc
    12421 ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga acacgtaccc
    12481 ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt cctggccgtc
    12541 ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc cagggcgtcg
    12601 gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt gggcgtcact
    12661 tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag cagtgcagcc
    12721 gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc gatctgtgcc
    12781 ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc ctcgcgcccg
    12841 ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc gaacacggtc
    12901 aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag gctacgcagg
    12961 cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt gctgcgggcc
    13021 aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc aagcatcctg
    13081 gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag cttggtgcag
    13141 ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt gctgacgcgg
    13201 gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct ccggttctag
    13261 tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca ggaaaagggc
    13321 agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc agaagacggc
    13381 tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat caaagtactt
    13441 tgatcccgag gggaaccctg tggttggcat gcacatacaa atggacgaac ggataaacct
//
