LOCUS       sequence-427368-       11469 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Vector control for Bicistronic BiFC.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11469)
  AUTHORS   Sivakumar P, Vaishnavi V, Gayatri K, Satheesh GR, Siddiqi I
  TITLE     Improved validation of protein interactions using bicistronic BiFC 
            (Bi2FC).
  JOURNAL   Physiol Mol Biol Plants. 2024 Jul;30(7):1047-1054. doi: 
            10.1007/s12298-024-01477-y. Epub 2024 Jul 2.
  PUBMED    39100877
REFERENCE   2  (bases 1 to 11469)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s12298-024-01477-y"; journalName: "Physiol Mol Biol 
            Plants"; date: "2024-07"; volume: "30"; issue: "7"; pages: 
            "1047-1054"
COMMENT     Sequence Label: pBi2FC-C-MGGAGM-P2A:mTagBFP2
FEATURES             Location/Qualifiers
     source          1..11469
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    54..78
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     266..288
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     280..297
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     281..297
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        405..1081
                     /label=CaMV 35S promoter (enhanced)
                     /note="cauliflower mosaic virus 35S promoter with a 
                     duplicated enhancer region"
     primer_bind     1018..1038
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1166..1417
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     1354..1375
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             1418..1444
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     protein_bind    1463..1487
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(1509..1533)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1541..1597
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             1598..1618
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1619..1639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             1640..1660
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     terminator      2391..2643
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(2676..2692)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2676..2692)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2689..2711)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2700..2716
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2724..2730,2731..2748,2749..2754))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2769..2790
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     terminator      complement(2826..3078)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(3229..4254)
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGAFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     promoter        complement(4306..4485)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    4991..5015
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             5536..6327
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      6573..7161
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7062..7081
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7315..7332
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    7347..7487
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(7413..7432)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     7573..7595
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      7831..8025
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8091..9164)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(9593..10222)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
       61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      301 aagcttgcgt attggctaga gcagcttgcc aacatggtgg agcacgacac tctcgtctac
      361 tccaagaata tcaaagatac agtctcagaa gaccaaaggg ctattgagac ttttcaacaa
      421 agggtaatat cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcaaa
      481 aggacagtag aaaaggaagg tggcacctac aaatgccatc attgcgataa aggaaaggct
      541 atcgttcaag atgcctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc
      601 atcgtggaaa aagaagacgt tccaaccacg tcttcaaagc aagtggattg atgtgaacat
      661 ggtggagcac gacactctcg tctactccaa gaatatcaaa gatacagtct cagaagacca
      721 aagggctatt gagacttttc aacaaagggt aatatcggga aacctcctcg gattccattg
      781 cccagctatc tgtcacttca tcaaaaggac agtagaaaag gaaggtggca cctacaaatg
      841 ccatcattgc gataaaggaa aggctatcgt tcaagatgcc tctgccgaca gtggtcccaa
      901 agatggaccc ccacccacga ggagcatcgt ggaaaaagaa gacgttccaa ccacgtcttc
      961 aaagcaagtg gattgatgtg atatctccac tgacgtaagg gatgacgcac aatcccacta
     1021 tccttcgcaa gacccttcct ctatataagg aagttcattt catttggaga ggacacgctg
     1081 aaatcaccag tgtattttta caacaattac caacaacaac aaacaacaaa caacattaca
     1141 attactattt acaatttcta gaatggacaa gcagaagaac ggcatcaagg ccaacttcaa
     1201 gatccgccac aacatcgagg acggcggcgt gcagctcgcc gaccactacc agcagaacac
     1261 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagct accagtccgc
     1321 cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
     1381 cgccgggatc actctcggca tggacgagct gtacaagtac ccatacgatg ttccagatta
     1441 cgctgggccc aggcctacta gtacaagttt gtacaaaaaa gcaggcttaa tgggtggtgc
     1501 aggaatgaac ccagctttct tgtacaaagt ggttctcgag gcaaccaact ttagcctgct
     1561 caagcaagca ggagatgttg aggaaaatcc tggcccccct aagaaaaaga gaaaggttcc
     1621 gaaaaagaaa agaaaggtgc caaaaaaaaa gagaaaggta atggtgagca agggcgagga
     1681 gcttatcaaa gagaatatgc acatgaagtt gtatatggag ggtactgtgg ataaccatca
     1741 ctttaaatgt acatcagaag gagagggaaa gccttacgag ggtactcaaa caatgagaat
     1801 taaggttgtg gagggtggtc ctcttccatt tgctttcgat atcttggcaa catcttttct
     1861 ctacggttca aaaactttca ttaaccatac acagggtatt ccagatttct tcaagcagtc
     1921 ttttccagag ggattcactt gggaaagagt tactacttat gaggatggtg gtgttcttac
     1981 tgcaactcaa gatacatctc ttcaggatgg atgcttgatc tataacgtta agatcagagg
     2041 agttaatttc acttcaaacg gacctgttat gcaaaagaaa acattgggtt gggaggcttt
     2101 tactgagaca ttgtacccag cagatggagg tttggaagga agaaacgata tggctcttaa
     2161 attggttgga ggttctcatc tcatcgcaaa tgctaaaact acatatagat caaagaaacc
     2221 tgctaagaac ctcaaaatgc caggagttta ttacgtggat tacagacttg aaagaattaa
     2281 agaggcaaac aacgaaactt atgttgaaca gcatgaggtt gctgtggcaa gatactgtga
     2341 tcttccttct aaactggggc acaaacttaa ttaagagctc gaatttcccc gatcgttcaa
     2401 acatttggca ataaagtttc ttaagattga atcctgttgc cggtcttgcg atgattatca
     2461 tataatttct gttgaattac gttaagcatg taataattaa catgtaatgc atgacgttat
     2521 ttatgagatg ggtttttatg attagagtcc cgcaattata catttaatac gcgatagaaa
     2581 acaaaatata gcgcgcaaac taggataaat tatcgcgcgc ggtgtcatct atgttactag
     2641 atcgggaatt ggttccggaa ccaattcgta atcatgtcat agctgtttcc tgtgtgaaat
     2701 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     2761 ggtgcctaat gagtgagcta actcacatta ggctgaatta ggcgcgccta tttctattaa
     2821 ttcccgatct agtaacatag atgacaccgc gcgcgataat ttatcctagt ttgcgcgcta
     2881 tattttgttt tctatcgcgt attaaatgta taattgcggg actctaatca taaaaaccca
     2941 tctcataaat aacgtcatgc attacatgtt aattattaca tgcttaacgt aattcaacag
     3001 aaattatatg ataatcatcg caagaccggc aacaggattc aatcttaaga aactttattg
     3061 ccaaatgttt gaacgatcgg ggaaattcgg gggatcgatc ccgcgaactg tggacgagaa
     3121 ctgtgccacc aagcgtaagg ccgttctctc gcatttgcct tgctaggctc gcgcgagttg
     3181 ctggctgagg cgttctcgaa atcagctctt gttcggtcgg catctactct attcctttgc
     3241 cctcggacga gtgctggggc gtcggtttcc actatcggcg agtacttcta cacagccatc
     3301 ggtccagacg gccgcgcttc tgcgggcgat ttgtgtacgc ccgacagtcc cggctccgga
     3361 tcggacgatt gcgtcgcatc gaccctgcgc ccaagctgca tcatcgaaat tgccgtcaac
     3421 caagctctga tagagttggt caagaccaat gcggagcata tacgcccgga gccgcggcga
     3481 tcctgcaagc tccggatgcc tccgctcgaa gtagcgcgtc tgctgctcca tacaagccaa
     3541 ccacggcctc cagaagaaga tgttggcgac ctcgtattgg gaatccccga acatcgcctc
     3601 gctccagtca atgaccgctg ttatgcggcc attgtccgtc aggacattgt tggagccgaa
     3661 atccgcgtgc acgaggtgcc ggacttcggg gcagtcctcg gcccaaagca tcagctcatc
     3721 gagagcctgc gcgacggacg cactgacggt gtcgtccatc acagtttgcc agtgatacac
     3781 atggggatca gcaatcgcgc atatgaaatc acgccatgta gtgtattgac cgattccttg
     3841 cggtccgaat gggccgaacc cgctcgtctg gctaagatcg gccgcagcga tcgcatccat
     3901 ggcctccgcg accggctgca gaacagcggg cagttcggtt tcaggcaggt cttgcaacgt
     3961 gacaccctgt gcacggcggg agatgcaata ggtcaggctc tcgctgaatg ccccaatgtc
     4021 aagcacttcc ggaatcggga gcgcggccga tgcaaagtgc cgataaacat aacgatcttt
     4081 gtagaaacca tcggcgcagc tatttacccg caggacatat ccacgccctc ctacatcgaa
     4141 gctgaaagca cgagattctt cgccctccga gagctgcatc aggtcggaga cgctgtcgaa
     4201 cttttcgatc agaaacttct cgacagacgt cgcggtgagt tcaggctttt tcatatctta
     4261 ttgccccccg ggatcagatc tggattgaga gtgaatatga gactctaatt ggataccgag
     4321 gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta gctgatagtg
     4381 accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt agctcattaa
     4441 actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca gttccaaacg
     4501 taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt aattctccgc
     4561 tcatgatctt gatcccctgc gccatcagat ccttggcggc aagaaagcca tccagtttac
     4621 tttgcagggc ttcccaacct taccagaggg cgccccagct ggcaattccg gttcgcttgc
     4681 tgtccataaa accgcccagt ctagctatcg ccatgtaagc ccactgcaag ctacctgctt
     4741 tctctttgcg cttgcgtttt cccttgtcca gatagcccag tagctgacat tcatccgggg
     4801 tcagcaccgt ttctgcggac tggctttcta cgtgttccgc ttcctttagc agcccttgcg
     4861 ccctgagtgc ttgcggcagc gtgaagctga tccgtcgaga ttaatagaaa taaattcagc
     4921 ctaattcggc gttaattcag tacattaaaa acgtccgcaa tgtgttatta agttgtctaa
     4981 gcgtcaattt gtttacacca caatatatcc tgccaccagc cagccaacag ctccccgacc
     5041 ggcagctcgg cacaaaatca ccactcgata caggcagccc atcagtccgg gacggcgtca
     5101 gcgggagagc cgttgtaagg cggcagactt tgctcatgtt accgatgcta ttcggaagaa
     5161 cggcaactaa gctgccgggt ttgaaacacg gatgatctcg cggagggtag catgttgatt
     5221 gtaacgatga cagagcgttg ctgcctgtga tcaattcggg cacgaaccca gtggacataa
     5281 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
     5341 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttgttatgac
     5401 tgtttttttg gggtacagtc tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg
     5461 ggtcgatgtt tgatgttatg gagcagcaac gatgttacgc agcagggcag tcgccctaaa
     5521 acaaagttaa acatcatggg ggaagcggtg atcgccgaag tatcgactca actatcagag
     5581 gtagttggcg tcatcgagcg ccatctcgaa ccgacgttgc tggccgtaca tttgtacggc
     5641 tccgcagtgg atggcggcct gaagccacac agtgatattg atttgctggt tacggtgacc
     5701 gtaaggcttg atgaaacaac gcggcgagct ttgatcaacg accttttgga aacttcggct
     5761 tcccctggag agagcgagat tctccgcgct gtagaagtca ccattgttgt gcacgacgac
     5821 atcattccgt ggcgttatcc agctaagcgc gaactgcaat ttggagaatg gcagcgcaat
     5881 gacattcttg caggtatctt cgagccagcc acgatcgaca ttgatctggc tatcttgctg
     5941 acaaaagcaa gagaacatag cgttgccttg gtaggtccag cggcggagga actctttgat
     6001 ccggttcctg aacaggatct atttgaggcg ctaaatgaaa ccttaacgct atggaactcg
     6061 ccgcccgact gggctggcga tgagcgaaat gtagtgctta cgttgtcccg catttggtac
     6121 agcgcagtaa ccggcaaaat cgcgccgaag gatgtcgctg ccgactgggc aatggagcgc
     6181 ctgccggccc agtatcagcc cgtcatactt gaagctagac aggcttatct tggacaagaa
     6241 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgtccacta cgtgaaaggc
     6301 gagatcacca aggtagtcgg caaataatgt ctagctagaa attcgttcaa gccgacgccg
     6361 cttcgcggcg cggcttaact caagcgttag atgcactaag cacataattg ctcacagcca
     6421 aactatcagg tcaagtctgc ttttattatt tttaagcgtg cataataagc cctacacaaa
     6481 ttgggagata tatcatgcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     6541 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     6601 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     6661 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtc
     6721 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     6781 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     6841 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     6901 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     6961 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     7021 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     7081 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     7141 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     7201 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     7261 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     7321 tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac gcatctgtgc
     7381 ggtatttcac accgcatatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     7441 agccagtata cactccgcta tcgctacgtg actgggtcat ggctgcgccc cgacacccgc
     7501 caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag
     7561 ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg
     7621 cgaggcaggg tgccttgatg tgggcgccgg cggtcgagtg gcgacggcgc ggcttgtccg
     7681 cgccctggta gattgcctgg ccgtaggcca gccatttttg agcggccagc ggccgcgata
     7741 ggccgacgcg aagcggcggg gcgtagggag cgcagcgacc gaagggtagg cgctttttgc
     7801 agctcttcgg ctgtgcgctg gccagacagt tatgcacagg ccaggcgggt tttaagagtt
     7861 ttaataagtt ttaaagagtt ttaggcggaa aaatcgcctt ttttctcttt tatatcagtc
     7921 acttacatgt gtgaccggtt cccaatgtac ggctttgggt tcccaatgta cgggttccgg
     7981 ttcccaatgt acggctttgg gttcccaatg tacgtgctat ccacaggaaa gagacctttt
     8041 cgaccttttt cccctgctag ggcaatttgc cctagcatct gctccgtaca ttaggaaccg
     8101 gcggatgctt cgccctcgat caggttgcgg tagcgcatga ctaggatcgg gccagcctgc
     8161 cccgcctcct ccttcaaatc gtactccggc aggtcatttg acccgatcag cttgcgcacg
     8221 gtgaaacaga acttcttgaa ctctccggcg ctgccactgc gttcgtagat cgtcttgaac
     8281 aaccatctgg cttctgcctt gcctgcggcg cggcgtgcca ggcggtagag aaaacggccg
     8341 atgccgggat cgatcaaaaa gtaatcgggg tgaaccgtca gcacgtccgg gttcttgcct
     8401 tctgtgatct cgcggtacat ccaatcagct agctcgatct cgatgtactc cggccgcccg
     8461 gtttcgctct ttacgatctt gtagcggcta atcaaggctt caccctcgga taccgtcacc
     8521 aggcggccgt tcttggcctt cttcgtacgc tgcatggcaa cgtgcgtggt gtttaaccga
     8581 atgcaggttt ctaccaggtc gtctttctgc tttccgccat cggctcgccg gcagaacttg
     8641 agtacgtccg caacgtgtgg acggaacacg cggccgggct tgtctccctt cccttcccgg
     8701 tatcggttca tggattcggt tagatgggaa accgccatca gtaccaggtc gtaatcccac
     8761 acactggcca tgccggccgg ccctgcggaa acctctacgt gcccgtctgg aagctcgtag
     8821 cggatcacct cgccagctcg tcggtcacgc ttcgacagac ggaaaacggc cacgtccatg
     8881 atgctgcgac tatcgcgggt gcccacgtca tagagcatcg gaacgaaaaa atctggttgc
     8941 tcgtcgccct tgggcggctt cctaatcgac ggcgcaccgg ctgccggcgg ttgccgggat
     9001 tctttgcgga ttcgatcagc ggccgcttgc cacgattcac cggggcgtgc ttctgcctcg
     9061 atgcgttgcc gctgggcggc ctgcgcggcc ttcaacttct ccaccaggtc atcacccagc
     9121 gccgcgccga tttgtaccgg gccggatggt ttgcgaccgc tcacgccgat tcctcgggct
     9181 tgggggttcc agtgccattg cagggccggc agacaaccca gccgcttacg cctggccaac
     9241 cgcccgttcc tccacacatg gggcattcca cggcgtcggt gcctggttgt tcttgatttt
     9301 ccatgccgcc tcctttagcc gctaaaattc atctactcat ttattcattt gctcatttac
     9361 tctggtagct gcgcgatgta ttcagatagc agctcggtaa tggtcttgcc ttggcgtacc
     9421 gcgtacatct tcagcttggt gtgatcctcc gccggcaact gaaagttgac ccgcttcatg
     9481 gctggcgtgt ctgccaggct ggccaacgtt gcagccttgc tgctgcgtgc gctcggacgg
     9541 ccggcactta gcgtgtttgt gcttttgctc attttctctt tacctcatta actcaaatga
     9601 gttttgattt aatttcagcg gccagcgcct ggacctcgcg ggcagcgtcg ccctcgggtt
     9661 ctgattcaag aacggttgtg ccggcggcgg cagtgcctgg gtagctcacg cgctgcgtga
     9721 tacgggactc aagaatgggc agctcgtacc cggccagcgc ctcggcaacc tcaccgccga
     9781 tgcgcgtgcc tttgatcgcc cgcgacacga caaaggccgc ttgtagcctt ccatccgtga
     9841 cctcaatgcg ctgcttaacc agctccacca ggtcggcggt ggcccatatg tcgtaagggc
     9901 ttggctgcac cggaatcagc acgaagtcgg ctgccttgat cgcggacaca gccaagtccg
     9961 ccgcctgggg cgctccgtcg atcactacga agtcgcgccg gccgatggcc ttcacgtcgc
    10021 ggtcaatcgt cgggcggtcg atgccgacaa cggttagcgg ttgatcttcc cgcacggccg
    10081 cccaatcgcg ggcactgccc tggggatcgg aatcgactaa cagaacatcg gccccggcga
    10141 gttgcagggc gcgggctaga tgggttgcga tggtcgtctt gcctgacccg cctttctggt
    10201 taagtacagc gataaccttc atgcgttccc cttgcgtatt tgtttattta ctcatcgcat
    10261 catatacgca gcgaccgcat gacgcaagct gttttactca aatacacatc acctttttag
    10321 acggcggcgc tcggtttctt cagcggccaa gctggccggc caggccgcca gcttggcatc
    10381 agacaaaccg gccaggattt catgcagccg cacggttgag acgtgcgcgg gcggctcgaa
    10441 cacgtacccg gccgcgatca tctccgcctc gatctcttcg gtaatgaaaa acggttcgtc
    10501 ctggccgtcc tggtgcggtt tcatgcttgt tcctcttggc gttcattctc ggcggccgcc
    10561 agggcgtcgg cctcggtcaa tgcgtcctca cggaaggcac cgcgccgcct ggcctcggtg
    10621 ggcgtcactt cctcgctgcg ctcaagtgcg cggtacaggg tcgagcgatg cacgccaagc
    10681 agtgcagccg cctctttcac ggtgcggcct tcctggtcga tcagctcgcg ggcgtgcgcg
    10741 atctgtgccg gggtgagggt agggcggggg ccaaacttca cgcctcgggc cttggcggcc
    10801 tcgcgcccgc tccgggtgcg gtcgatgatt agggaacgct cgaactcggc aatgccggcg
    10861 aacacggtca acaccatgcg gccggccggc gtggtggtgt cggcccacgg ctctgccagg
    10921 ctacgcaggc ccgcgccggc ctcctggatg cgctcggcaa tgtccagtag gtcgcgggtg
    10981 ctgcgggcca ggcggtctag cctggtcact gtcacaacgt cgccagggcg taggtggtca
    11041 agcatcctgg ccagctccgg gcggtcgcgc ctggtgccgg tgatcttctc ggaaaacagc
    11101 ttggtgcagc cggccgcgtg cagttcggcc cgttggttgg tcaagtcctg gtcgtcggtg
    11161 ctgacgcggg catagcccag caggccagcg gcggcgctct tgttcatggc gtaatgtctc
    11221 cggttctagt cgcaagtatt ctactttatg cgactaaaac acgcgacaag aaaacgccag
    11281 gaaaagggca gggcggcagc ctgtcgcgta acttaggact tgtgcgacat gtcgttttca
    11341 gaagacggct gcactgaacg tcagaagccg actgcactat agcagcggag gggttggatc
    11401 aaagtacttt gatcccgagg ggaaccctgt ggttggcatg cacatacaaa tggacgaacg
    11461 gataaacct
//
