LOCUS       sequence-42737-e         571 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 571)
  AUTHORS   Korneel Hens, Jean-Daniel Feuz, Alina Isakova, Antonina Lagovitina, 
            Andreas Massouras, Julien Bryois, Patrick Callaerts, Susan E. 
            Celniker, Bart Deplancke
  TITLE     Automated protein-DNA interaction screening of Drosophila regulatory
            elements
  JOURNAL   Nat Methods. 2011 Oct 30;8(12):1065-70.
  PUBMED    22037703
REFERENCE   2  (bases 1 to 571)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-10-30"; volume: "8(12)"; pages: "1065-70"
COMMENT     Sequence Label: KHBD00416
FEATURES             Location/Qualifiers
     source          1..571
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    63..87
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(427..526)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(543..562)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(544..562)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tattttgact gatagtgacc tgttcgttgc aacaaattga tgagcaatgc ttttttataa
       61 tgccaacttt gtacaaaaaa gttggcacca tgtctgataa gccaaaacgc ccactctccg
      121 cctacatgct gtggctcaac agtgcccgcg agtcgatcaa gcgcgagaat cccggcatca
      181 aggtcaccga ggtggccaag cgcggtggtg aattatggag ggcaatgaag gacaagtctg
      241 agtgggaggc caaggctgct aaggccaagg acgactacga tcgggcggtc aaggagttcg
      301 aggccaatgg tggcagcagt gctgccaacg gtggtggagc caagaagcgc gcgaaaccag
      361 cgaaaaaggt ggcgaagaag agcaagaagg aggagtccga cgaggacgac gatgatgaga
      421 gcgagttgcc aactttcttg tacaaagttg gcattataag aaagcattgc ttatcaattt
      481 gttgcaacga acaggtcact atcagtcaaa ataaaatcat tatttgccat ccagctgata
      541 tcccctatag tgagtcgtat tacatggtca t
//
