LOCUS       sequence-42767-e         908 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 908)
  AUTHORS   Shaw RJ, Kosmatka M, Bardeesy N, Hurley RL, Witters LA, DePinho RA, 
            Cantley LC
  TITLE     The tumor suppressor LKB1 kinase directly activates AMP-activated 
            kinase and regulates apoptosis in response to energy stress.
  JOURNAL   Proc Natl Acad Sci U S A 2004 Mar 9;101(10):3329-35. Epub 2004 Feb 
            25.
  PUBMED    14985505
REFERENCE   2  (bases 1 to 908)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "2004-03-9"; volume: "101(10)"; pages: 
            "3329-35. Epub 2004 Feb 25"
COMMENT     Sequence Label: pcDNA3-FLAG-KD LKB1
FEATURES             Location/Qualifiers
     source          1..908
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagcttagc caccatggac tacaaagacg atgacgataa agcaagggat
      121 ccactagtaa cggccgccag tgtgctggaa ttctccagca tggaggtggt ggacccgcag
      181 cagctgggca tgttcacgga gggcgagctg atgtcggtgg gtatggacac gttcatccac
      241 cgcatcgact ccaccgaggt catctaccag ccgcgccgca agcgggccaa gctcatcggc
      301 aagtacctga tgggggacct gctgggggaa ggctcttacg gcaaggtgaa ggaggtgctg
      361 gactcggaga cgctgtgcag gagggccgtc atcatcctca agaagaagaa gttgcgaagg
      421 atccccaacg gggaggccaa cgtgaagaag gaaattcaac tactgaggag gttacggcac
      481 aaaaatgtca tccagctggt ggatgtgtta tacaacgaag agaagcagaa aatgtatatg
      541 gtgatggagt actgcgtgtg tggcatgcag gaaatgctgg acagcgtgcc ggagaagcgt
      601 ttcccagtgt gccaggccca cgggtacttc tgtcagctga ttgacggcct ggagtacctg
      661 catagccagg gcattgtgca caaggacatc aagccgggga acctgctgct caccaccggt
      721 ggcaccctca aaatctccga cctgggcgtg gccgaggcac tgcacccgtt cgcggcggac
      781 gacacctgcc ggaccagcca gggctccccg gctttccagc cgcccgagat tgccaacggc
      841 ctggacacct tctccggctt caaggtggac atctggtcgg ctggggtcac cctctacaac
      901 atcaccac
//
