LOCUS       sequence-42768-e         566 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent expression of ChR2 and PSAM-GlyR (L141F,Y115F) 
            neuronal inhibitor, with IRES-EGFP marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Magnus CJ, Lee PH, Atasoy D, Su HH, Looger LL, Sternson SM
  TITLE     Chemical and genetic engineering of selective ion channel-ligand 
            interactions.
  JOURNAL   Science. 2011 Sep 2;333(6047):1292-6.
  PUBMED    21885782
REFERENCE   2  (bases 1 to 566)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-09-2"; volume: "333(6047)"; pages: "1292-6"
COMMENT     Sequence Label: rAAV-CAG::FLEX-rev:ChR2HA:2a:PSAML141F,Y115F:GlyR
FEATURES             Location/Qualifiers
     source          1..566
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             45..71
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             45..71
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             72..98
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             105..158
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tcgagactct cgtcgaagac gaagccgagg ccggagccgt ggcatacccc tacgacgtgc
       61 ccgactacgc ctatccatat gatgtcccag actatgctgc tagcgaagga cgtggatctc
      121 tgctgacctg tggagatgtg gaagagaatc ccggaccatc gcgaatgcgc tgttctccag
      181 gcggcgtgtg gctcgccctg gctgcttccc ttctgcacgt tagcctgcag ggtgagttcc
      241 agcgcaaact gtataaggag cttgttaaga attataaccc cctggagcgg ccggtcgcaa
      301 atgattccca gccactgaca gtgtacttca gcctctcctt gctgcagatc atggacgtgg
      361 atgaaaagaa ccaggtgctg accactaata tttggttgca gatgtcctgg accgatcact
      421 acttgcagtg gaatgtgagc gaatacccag gtgtaaagac tgtaagattc cctgacggcc
      481 aaatctggaa accagatatc ctgctgttca acagcgcaga cgaaaggttt gatgcaacat
      541 ttcacaccaa cgtgttggtc aattct
//
