LOCUS       sequence-42816-e         834 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Wang L, Jin Q, Lee JE, Su IH, Ge K
  TITLE     Histone H3K27 methyltransferase Ezh2 represses Wnt genes to 
            facilitate adipogenesis.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Apr 20. 107(16):7317-22.
  PUBMED    20368440
REFERENCE   2  (bases 1 to 834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Apr 20. 107(16):7317-22."
COMMENT     Sequence Label: WZLneo-PPAR-gamma-2
FEATURES             Location/Qualifiers
     source          1..834
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      20..29
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             29..52
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             53..79
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 ggcgccggcc ggatcccggg ccaccatgga ctacaaggac gacgatgaca agtaccctta
       61 tgatgtccct gattatgcct ccggtgaaac tctgggagat tctcctgttg acccagagca
      121 tggtgccttc gctgatgcac tgcctatgag cacttcacaa gaaattacca tggttgacac
      181 agagatgcca ttctggccca ccaacttcgg aatcagctct gtggacctct ccgtgatgga
      241 agaccactcg cattcctttg acatcaagcc ctttaccaca gttgatttct ccagcatttc
      301 tgctccacac tatgaagaca ttccattcac aagagctgac ccaatggttg ctgattacaa
      361 atatgacctg aagctccaag aataccaaag tgcgatcaaa gtagaacctg catctccacc
      421 ttattattct gaaaagaccc agctctacaa caggcctcat gaagaacctt ctaactccct
      481 catggccatt gagtgccgag tctgtgggga taaagcatca ggcttccact atggagttca
      541 tgcttgtgaa ggatgcaagg gttttttccg aagaaccatc cgattgaagc ttatttatga
      601 taggtgtgat cttaactgcc ggatccacaa aaaaagtaga aataaatgtc agtactgtcg
      661 gtttcagaag tgccttgctg tggggatgtc tcacaatgcc atcaggtttg ggcggatgcc
      721 acaggccgag aaggagaagc tgttggcgga gatctccagt gatatcgacc agctgaaccc
      781 agagtctgct gatctgcgag ccctggcaaa gcatttgtat gactcataca taaa
//
