LOCUS       sequence-42820-e         862 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 862)
  TITLE     Yaffe lab HA-tagged 14-3-3 plasmids
REFERENCE   2  (bases 1 to 862)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pcDNA3-HA-14-3-3 gamma
FEATURES             Location/Qualifiers
     source          1..862
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     45..64
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        45..63
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             86..112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             86..112
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             92..118
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 ctctctggct actgagaacc cactgcttac tggcttatcg aaattaatac gactcactat
       61 agggagaccc aagcttacca tggcctaccc ctacgacgtg cccgactacg cctccctcgg
      121 atccccggaa ttcatggtgg accgcgagca actagtgcag aaagcccggc tggccgagca
      181 ggcggagcgc tacgacgata tggccgcggc catgaagaac gtgacagagc tgaatgaacc
      241 actgtccaac gaggaacgga acctcctgtc ggtggcctac aagaacgtgg ttggggctcg
      301 ccgctcctcc tggagggtca tcagcagcat cgagcagaag acgtctgcgg atggcaacga
      361 gaagaagata gagatggtcc gagcctaccg ggaaaagatt gagaaggagc tggaggccgt
      421 gtgccaggac gtgctgagcc tgctggacaa ctacctgatc aagaactgca gcgagaccca
      481 gtacgagagc aaggtgttct acctgaagat gaaaggggac tattaccgct acctggctga
      541 agtggccact ggggagaaaa gggcgaccgt ggtggagtcg tccgagaagg cctacagcga
      601 agcccacgag atcagcaagg agcacatgca gcccacccac cccatccggc tgggcctggc
      661 gctcaactac tccgttttct actacgagat ccagaacgcc ccagagcaag cctgccacct
      721 ggccaagacc gccttcgatg acgccatcgc cgagctcgac actctgaacg aggactccta
      781 caaggactcc actctgatca tgcagctgct ccgagacaac ctaacgctct ggacgagcga
      841 ccagcaagac gacgacggcg gc
//
