LOCUS       sequence-42821-e         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Yaffe MB, Rittinger K, Volinia S, Caron PR, Aitken A, Leffers H, 
            Gamblin SJ, Smerdon SJ, Cantley LC
  TITLE     The structural basis for 14-3-3:phosphopeptide binding specificity.
  JOURNAL   Cell. 1997 Dec 26. 91(7):961-71.
  PUBMED    9428519
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 1997 
            Dec 26. 91(7):961-71."
COMMENT     Sequence Label: pGEX-4T1-14-3-3 epsilon GST
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(120..137)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(144..797)
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     complement(164..186)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     primer_bind     complement(809..831)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    820..836
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(844..850,851..866,867..872))
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
ORIGIN
        1 actgtcagct ccacgtccat tcctgctact ttcttcattg attccaccat ttcgtcgtat
       61 cgctcggcct gctctgccag cttcgcctgg tacaccagat cctcccgatc atccatggtg
      121 gatccacgcg gaaccagatc cgattttgga ggatggtcgc caccaccaaa cgtggcttgc
      181 cagccctgca aaggccatgc tatatacttg ctggatttca agtacttatc aatttgtggg
      241 atagcttcaa tacgtttttt aaaacaaact aattttggga acgcatccag gcacattggg
      301 tccatgtata aaacaacatc aagagcgtca tacaacatga agtcaggatg ggttacatga
      361 tcaccattta aatatgtttt atgacataaa cgatcttcga acattttcag catttcaggt
      421 agcttgctaa gaaaatcaac tttgagagtt tcaaagtctt tactatatgc aattctcgaa
      481 acaccgtatc taatatccaa aaccgctcct tcaagcattg aaatctctgc acgctctttt
      541 ggacaaccac ccaacatgtt gtgcttgtca gctatataac gtatgatggc catagactgt
      601 gttaatttaa catcaccatc aatataataa ggaagattgg gaaactccaa acccaattca
      661 aactttttgt ttcgccattt atcaccttca tcgcgctcat acaaatgctc ttcatatttt
      721 tcttcaagat attccaaaag aagtcgagtg ggttgcacaa ggcccttaat tttccaataa
      781 cctagtatag gggacatgaa tactgtttcc tgtgtgaaat tgttatccgc tcacaattcc
      841 acacattata cgagccgatg attaattgtc aacagctcat ttca
//
