LOCUS       sequence-42834-e         639 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Cho YJ, Cunnick JM, Yi SJ, Kaartinen V, Groffen J, Heisterkamp N
  TITLE     Abr and Bcr, two homologous Rac GTPase-activating proteins, control 
            multiple cellular functions of murine macrophages.
  JOURNAL   Mol Cell Biol. 2007 Feb;27(3):899-911. Epub 2006 Nov 20.
  PUBMED    17116687
REFERENCE   2  (bases 1 to 639)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol."; date: "2007-02"; volume: "27(3)"; pages: "899-911. Epub 2006
            Nov 20"
COMMENT     Sequence Label: pCDNA3.1Xpress-Bcr-HisB
FEATURES             Location/Qualifiers
     source          1..639
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             104..121
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             125..157
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(161..169,170..184)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 tagagaaccc actgtcttac tggcttatcg aaattaatac gcctttntat agggagaccc
       61 aagctggcta gcgtttaaac ttaancttac catggggggt tctcatcatc atcatcatca
      121 tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg acgatgacga
      181 taaggtacct aaggatccag tgtggtggaa ttcggtggac ccggtgggct tcgcggaggc
      241 gtggaaggcg cagttcccgg actcagagcc cccgcgcatg gagctgcgct cagtgggcga
      301 catcgagcag gagctggagc gctgcaaggc ctccattcgg cgcctggagc aggaggtgaa
      361 ccaggagcgc ttccgcatga tctacctgca gacgttgctg gccaaggaaa agaagagcta
      421 tgaccggcag cgatggggct tccggcgcgc ggcgcaggcc cccgacggcg cctccgagcc
      481 ccgagcgtcc gcgtcgcgcc cgcagccagc gcccgccgac ggagccgacc cgccgcccgc
      541 cgaggagccc gaggcccggc ccgacggcga gggttctccg ggtaaggcca ggcccgggac
      601 cgcccgcagg cccggggcag ccgcgtcggg ggaacggga
//
