LOCUS       sequence-42835-e         839 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Sondheimer N, Lindquist S
  TITLE     Rnq1: an epigenetic modifier of protein function in yeast.
  JOURNAL   Mol Cell 2000 Jan;5(1):163-72.
  PUBMED    10678178
REFERENCE   2  (bases 1 to 839)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell"; 
            date: "2000-01"; volume: "5(1)"; pages: "163-72"
COMMENT     Sequence Label: pRS316CUP RNQ CFP
FEATURES             Location/Qualifiers
     source          1..839
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(24..734)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIKAH
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(64..84)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     679..707
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gggcgaaatt ggagctctta ctatttgtat agttcatcca tgccatgtgt aatcccagca
       61 gctgttacaa actcaagaag gaccatgtgg tctctctttt cgttgggatc tttcgaaagg
      121 gcagattgtg tggacaggta atggttgtct ggtaaaagga cagggccatc gccaattgga
      181 gtattttgtt gataatggtc tgctagttga acgcttccat cttcaatgtt gtgtctaatt
      241 ttgaaatgag ctttgattcc attcttttgt ttgtctgcag tgatgtatac attgtgtgaa
      301 atatagttgt attccaattt gtgtccaaga atgtttccat cttctttaaa atcaatacct
      361 tttaactcga ttctattaac aagggtatca ccttcaaact tgacttcagc acgtgtcttg
      421 tagttcccgt catctttgaa aaatatagtt ctttcctgta cataaccttc gggcatggca
      481 ctcttgaaaa agtcatgccg tttcatatga tctgggtatc ttgaaaagca ttgaacaccc
      541 caagtcaaag tagtgacaag tgttggccat ggaacaggta gttttccagt agtgcaaata
      601 aatttaaggg taagttttcc gtatgttgca tcaccttcac cctctccact gacagaaaat
      661 ttgtgcccat taacatcacc atctaattca acaagaattg ggacaactcc agtgaaaagt
      721 tcttctcctt tactccgcgg gtagcggttc ttgagaaaag tgccagaaaa attgaaggat
      781 tcgtgctgtc cattggagtt ctggtttccg ccgtattgcg gtcttccgta ctcattgga
//
