LOCUS       sequence-428497-        3294 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcription factor REST with a specific barcode assigned..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3294)
  TITLE     The Immune Top TF library is a collection of Immune-related human TF
            ORFs in LV vectors. For each TF, an 8 nt seq is assigned, allowing 
            simple identification during single-cell RNA-seq.
REFERENCE   2  (bases 1 to 3294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SFFV-REST-Brd 422
FEATURES             Location/Qualifiers
     source          1..3294
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2582..2599
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atggccaccc aggtaatggg gcagtcttct ggaggaggag ggctgtttac cagcagtggc
       61 aacattggaa tggccctgcc taacgacatg tatgacttgc atgacctttc caaagctgaa
      121 ctggccgcac ctcagcttat tatgctggca aatgtggcct taactgggga agtaaatggc
      181 agctgctgtg attacctggt cggtgaagaa agacagatgg cagaactgat gccggttggg
      241 gataacaact tttcagatag tgaagaagga gaaggacttg aagagtctgc tgatataaaa
      301 ggtgaacctc atggactgga aaacatggaa ctgagaagtt tggaactcag cgtcgtagaa
      361 cctcagcctg tatttgaggc atcaggtgct ccagatattt acagttcaaa taaagatctt
      421 ccccctgaaa cacctggagc ggaggacaaa ggcaagagct cgaagaccaa accctttcgc
      481 tgtaagccat gccaatatga agcagaatct gaagaacagt ttgtgcatca catcagagtt
      541 cacagtgcta agaaattttt tgtggaagag agtgcagaga agcaggcaaa agccagggaa
      601 tctggctctt ccactgcaga agagggagat ttctccaagg gccccattcg ctgtgaccgc
      661 tgcggctaca atactaatcg atatgatcac tatacagcac acctgaaaca ccacaccaga
      721 gctggggata atgagcgagt ctacaagtgt atcatttgca catacacaac agtgagcgag
      781 tatcactgga ggaaacattt aagaaaccat tttccaagga aagtatacac atgtggaaaa
      841 tgcaactatt tttcagacag aaaaaacaat tatgttcagc atgttagaac tcatacagga
      901 gaacgcccat ataaatgtga actttgtcct tactcaagtt ctcagaagac tcatctaact
      961 agacatatgc gtactcattc aggtgagaag ccatttaaat gtgatcagtg cagttatgtg
     1021 gcctctaatc aacatgaagt aacccgccat gcaagacagg ttcacaatgg gcctaaacct
     1081 cttaattgcc cacactgtga ttacaaaaca gcagatagaa gcaacttcaa aaaacatgta
     1141 gagctacatg tgaacccacg gcagttcaat tgccctgtat gtgactatgc agcttccaag
     1201 aagtgtaatc tacagtatca cttcaaatct aagcatccta cttgtcctaa taaaacaatg
     1261 gatgtctcaa aagtgaaact aaagaaaacc aaaaaacgag aggctgactt gcctgataat
     1321 attaccaatg aaaaaacaga aatagaacaa acaaaaataa aaggggatgt ggctggaaag
     1381 aaaaatgaaa agtccgtcaa agcagagaaa agagatgtct caaaagagaa aaagccttct
     1441 aataatgtgt cagtgatcca ggtgactacc agaactcgaa aatcagtaac agaggtgaaa
     1501 gagatggatg tgcatacagg aagcaattca gaaaaattca gtaaaactaa gaaaagcaaa
     1561 aggaagctgg aagttgacag ccattcttta catggtcctg tgaatgatga ggaatcttca
     1621 acaaaaaaga aaaagaaggt agaaagcaaa tccaaaaata atagtcagga agtgccaaag
     1681 ggtgacagca aagtggagga gaataaaaag caaaatactt gcatgaaaaa aagtacaaag
     1741 aagaaaactc tgaaaaataa atcaagtaag aaaagcagta agcctcctca gaaggaacct
     1801 gttgagaagg gatctgctca gatggaccct cctcagatgg ggcctgctcc cacagaggcg
     1861 gttcagaagg ggcccgttca ggtggagccg ccacctccca tggagcatgc tcagatggag
     1921 ggtgcccaga tacggcctgc tcctgacgag cctgttcaga tggaggtggt tcaggagggg
     1981 cctgctcaga aggagctgct gcctcccgtg gagcctgctc agatggtggg tgcccaaatt
     2041 gtacttgctc acatggagct gcctcctccc atggagactg ctcagacgga ggttgcccaa
     2101 atggggcctg ctcccatgga acctgctcag atggaggttg cccaggtaga atctgctccc
     2161 atgcaggtgg tccagaagga gcctgttcag atggagctgt ctcctcccat ggaggtggtc
     2221 cagaaggagc ctgttcagat agagctgtct cctcccatgg aggtggtcca gaaggaacct
     2281 gttaagatag agctgtctcc tcccatagag gtggtccaga aggagcctgt tcagatggag
     2341 ttgtctcctc ccatgggggt ggttcagaag gagcctgctc agagggagcc acctcctccc
     2401 agagagcctc cccttcacat ggagccaatt tccaaaaagc ctcctctccg aaaagataaa
     2461 aaggaaaagt ctaacatgca gagtgaaagg gcacggaagg agcaagtcct tattgaagtt
     2521 ggcttagtgc ctgttaaaga tagctggctt ctaaaggaaa gtgtaagcac agaggatctc
     2581 tcaccaccat caccaccact gccaaaggaa aatttaagag aagaggcatc aggagaccaa
     2641 aaattactca acacaggtga aggaaataaa gaagcccctc ttcagaaagt aggagcagaa
     2701 gaggcagatg agagcctacc tggtcttgct gctaatatca acgaatctac ccatatttca
     2761 tcctctggac aaaacttgaa tacgccagag ggtgaaactt taaatggtaa acatcagact
     2821 gacagtatag tttgtgaaat gaaaatggac actgatcaga acacaagaga gaatctcact
     2881 ggtataaatt caacagttga agaaccagtt tcaccaatgc ttcccccttc agcagtagaa
     2941 gaacgtgaag cagtgtccaa aactgcactg gcatcacctc ctgctacaat ggcagcaaat
     3001 gagtctcagg aaattgatga agatgaaggc atccacagcc atgaaggaag tgacctaagt
     3061 gacaacatgt cagagggtag tgatgattct ggattgcatg gggctcggcc agttccacaa
     3121 gaatctagca gaaaaaatgc aaaggaagcc ttggcagtca aagcggctaa gggagatttt
     3181 gtttgtatct tctgtgatcg ttctttcaga aagggaaaag attacagcaa acacctcaat
     3241 cgccatttgg ttaatgtgta ctatcttgaa gaagcagctc aagggcagga gtag
//
