LOCUS       sequence-42856-e       12020 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12020)
  AUTHORS   Wang JW, Beck ES, McCabe BD
  TITLE     A modular toolset for recombination transgenesis and neurogenetic 
            analysis of Drosophila.
  JOURNAL   PLoS One. 2012;7(7):e42102. Epub 2012 Jul 25.
  PUBMED    22848718
REFERENCE   2  (bases 1 to 12020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(7)"; pages: "e42102. Epub 2012 Jul 25"
COMMENT     Sequence Label: pBID-UASC-MRG
FEATURES             Location/Qualifiers
     source          1..12020
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     623..642
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        623..641
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     gene            666..4802
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(675..693)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(1145..1216,1618..1891,1966..2620,2682..2997,3218..3349,
                     3420..4034)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLRPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     protein_bind    4809..4842
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    4855..5285
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    5331..5425
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     protein_bind    5449..5543
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     CDS             5786..5875
                     /codon_start=1
                     /product="3 tandem Myc epitope tags"
                     /label=3xMyc
                     /translation="EQKLISEEDLEQKLISEEDLEQKLISEEDL"
     protein_bind    6623..6747
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(6772..6777,6778..6795,6796..6802)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     CDS             6856..7515
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(6922..6941)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     CDS             7857..8162
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     8041..8060
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
     protein_bind    complement(8203..8327)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     intron          8457..8522
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8652..8672
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8944..9078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8981..9000)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9035..9054
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    9080..9510
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     protein_bind    9544..9613
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     promoter        complement(9832..9850)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9871..9887)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9871..9887)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9884..9906)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9895..9911
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9919..9925,9926..9943,9944..9949))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9964..9985
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10102..10119)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10273..10861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10353..10372)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11032..11823,11824..11892))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11655..11674
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11893..11997)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgaat tgtaatacga ctcactatag ggcgaattgg gtacgtaccg
      661 ggcccctagt atgtatgtaa gttaataaaa cccatttttg cggaaagtag ataaaaaaaa
      721 catttttttt ttttactgca ctggatatca ttgaacttat ctgatcagtt ttaaatttac
      781 ttcgatccaa gggtatttga tgtaccaggt tctttcgatt acctctcact caaaatgaca
      841 ttccactcaa agtcagcgct gtttgcctcc ttctctgtcc acagaaatat cgccgtctct
      901 ttcgccgctg cgtccgctat ctctttcgcc accgtttgta gcgttacgta gcgtcaatgt
      961 ccgccttcag ttgcattttg tcagcggttt cgtgacgaag ctccaagcgg tttacgccat
     1021 caattaaaca caaagtgctg tgccaaaact cctctcgctt cttatttttg tttgtttttt
     1081 gagtgattgg ggtggtgatt ggttttgggt gggtaagcag gggaaagtgt gaaaaatccc
     1141 ggcaatgggc caagaggatc aggagctatt aattcgcgga ggcagcaaac acccatctgc
     1201 cgagcatctg aacaatgtga gtagtacatg tgcatacatc ttaagttcac ttgatctata
     1261 ggaactgcga ttgcaacatc aaattgtctg cggcgtgaga actgcgaccc acaaaaatcc
     1321 caaaccgcaa ttgcacaaac aaatagtgac acgaaacaga ttattctggt agctgttctc
     1381 gctatataag acaatttttg agatcatatc atgatcaaga catctaaagg cattcatttt
     1441 cgactatatt cttttttaca aaaaatataa caaccagata ttttaagctg atcctagatg
     1501 cacaaaaaat aaataaaagt ataaacctac ttcgtaggat acttcggggt actttttgtt
     1561 cggggttaga tgagcataac gcttgtagtt gatatttgag atcccctatc attgcagggt
     1621 gacagcggag cggcttcgca gagctgcatt aaccagggct tcgggcaggc caaaaactac
     1681 ggcacgctcc ggccacccag tccgccggag gactccggtt cagggagcgg ccaactagcc
     1741 gagaacctca cctatgcctg gcacaatatg gacatctttg gggcggtcaa tcagccgggc
     1801 tccggatggc ggcagctggt caaccggaca cgcggactat tctgcaacga gcgacacata
     1861 ccggcgccca ggaaacattt gctcaagaac ggtgagtttc tattcgcagt cggctgatct
     1921 gtgtgaaatc ttaataaagg gtccaattac caatttgaaa ctcagtttgc ggcgtggcct
     1981 atccgggcga acttttggcc gtgatgggca gttccggtgc cggaaagacg accctgctga
     2041 atgcccttgc ctttcgatcg ccgcagggca tccaagtatc gccatccggg atgcgactgc
     2101 tcaatggcca acctgtggac gccaaggaga tgcaggccag gtgcgcctat gtccagcagg
     2161 atgacctctt tatcggctcc ctaacggcca gggaacacct gattttccag gccatggtgc
     2221 ggatgccacg acatctgacc tatcggcagc gagtggcccg cgtggatcag gtgatccagg
     2281 agctttcgct cagcaaatgt cagcacacga tcatcggtgt gcccggcagg gtgaaaggtc
     2341 tgtccggcgg agaaaggaag cgtctggcat tcgcctccga ggcactaacc gatccgccgc
     2401 ttctgatctg cgatgagccc acctccggac tggactcatt taccgcccac agcgtcgtcc
     2461 aggtgctgaa gaagctgtcg cagaagggca agaccgtcat cctgaccatt catcagccgt
     2521 cttccgagct gtttgagctc tttgacaaga tccttctgat ggccgagggc agggtagctt
     2581 tcttgggcac tcccagcgaa gccgtcgact tcttttccta gtgagttcga tgtgtttatt
     2641 aagggtatct agcattacat tacatctcaa ctcctatcca gcgtgggtgc ccagtgtcct
     2701 accaactaca atccggcgga cttttacgta caggtgttgg ccgttgtgcc cggacgggag
     2761 atcgagtccc gtgatcggat cgccaagata tgcgacaatt ttgctattag caaagtagcc
     2821 cgggatatgg agcagttgtt ggccaccaaa aatttggaga agccactgga gcagccggag
     2881 aatgggtaca cctacaaggc cacctggttc atgcagttcc gggcggtcct gtggcgatcc
     2941 tggctgtcgg tgctcaagga accactcctc gtaaaagtgc gacttattca gacaacggtg
     3001 agtggttcca gtggaaacaa atgatataac gcttacaatt cttggaaaca aattcgctag
     3061 attttagtta gaattgcctg attccacacc cttcttagtt tttttcaatg agatgtatag
     3121 tttatagttt tgcagaaaat aaataaattt catttaactc gcgaacatgt tgaagatatg
     3181 aatattaatg agatgcgagt aacattttaa tttgcagatg gttgccatct tgattggcct
     3241 catctttttg ggccaacaac tcacgcaagt gggcgtgatg aatatcaacg gagccatctt
     3301 cctcttcctg accaacatga cctttcaaaa cgtctttgcc acgataaatg taagtcttgt
     3361 ttagaataca tttgcatatt aataatttac taactttcta atgaatcgat tcgatttagg
     3421 tgttcacctc agagctgcca gtttttatga gggaggcccg aagtcgactt tatcgctgtg
     3481 acacatactt tctgggcaaa acgattgccg aattaccgct ttttctcaca gtgccactgg
     3541 tcttcacggc gattgcctat ccgatgatcg gactgcgggc cggagtgctg cacttcttca
     3601 actgcctggc gctggtcact ctggtggcca atgtgtcaac gtccttcgga tatctaatat
     3661 cctgcgccag ctcctcgacc tcgatggcgc tgtctgtggg tccgccggtt atcataccat
     3721 tcctgctctt tggcggcttc ttcttgaact cgggctcggt gccagtatac ctcaaatggt
     3781 tgtcgtacct ctcatggttc cgttacgcca acgagggtct gctgattaac caatgggcgg
     3841 acgtggagcc gggcgaaatt agctgcacat cgtcgaacac cacgtgcccc agttcgggca
     3901 aggtcatcct ggagacgctt aacttctccg ccgccgatct gccgctggac tacgtgggtc
     3961 tggccattct catcgtgagc ttccgggtgc tcgcatatct ggctctaaga cttcgggccc
     4021 gacgcaagga gtagccgaca tatatccgaa ataactgctt gttttttttt tttaccatta
     4081 ttaccatcgt gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc
     4141 aataaaaaca agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag
     4201 tagactttgg atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa
     4261 actcgtttat cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc
     4321 gcgcgccctc ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt
     4381 ctcccacccg ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg
     4441 ttagttccgc gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag
     4501 aaattagtaa taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt
     4561 ccctcacagc tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga
     4621 cctgttcgga gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct
     4681 tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa
     4741 taacaactat aataataaga atacatttaa tttagaaaat gcttggattt cactggaact
     4801 aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg atcctggcca
     4861 cgtaataagt gtgcgttgaa tttattcgca aaaacattgc atattttcgg caaagtaaaa
     4921 ttttgttgca taccttatca aaaaataagt gctgcatact ttttagagaa accaaataat
     4981 tttttattgc atacccgttt ttaataaaat acattgcata ccctctttta ataaaaaata
     5041 ttgcatactt tgacgaaaca aattttcgtt gcatacccaa taaaagatta ttatattgca
     5101 tacccgtttt taataaaata cattgcatac cctcttttaa taaaaaatat tgcatacgtt
     5161 gacgaaacaa attttcgttg catacccaat aaaagattat tatattgcat accttttctt
     5221 gccataccat ttagccgatc aattgtgctc ggcaacagta tatttgtggt gtgccaacca
     5281 acaacagatc caagctggcc gcggctcgat ccgcttgcat gcctgcaggt cggagtactg
     5341 tcctccgagc ggagtactgt cctccgagcg gagtactgtc ctccgagcgg agtactgtcc
     5401 tccgagcgga gtactgtcct ccgagcggaa gcttgcatgc ctgcaggtcg gagtactgtc
     5461 ctccgagcgg agtactgtcc tccgagcgga gtactgtcct ccgagcggag tactgtcctc
     5521 cgagcggagt actgtcctcc gagcggagac tctagcgagc tcgcccgggg atcgagcgca
     5581 gcggtataaa agggcgcggg gtggctgaga gcatcagttg tgaatgaatg ttcgagccga
     5641 gcagacgtgc cgctgccttc gttaatatcc tttgaataag ccaactttga atcacaagac
     5701 gcataccaaa cgaattcgca gatctgcggc cgcggctcga gggccggatc tgcaggtcga
     5761 ccagctggcc aagcttatca acatggagca gaaactcatc tctgaagaag atctggaaca
     5821 aaagttgatt tcagaagaag atctggaaca gaagctcatc tctgaggaag atctgtctag
     5881 agtgagcaag ggcgaggagc tgattaagga gaacatgcac atgaagctgt acatggaggg
     5941 caccgtgaac aaccaccact tcaagtgcac atccgagggc gaaggcaagc cctacgaggg
     6001 cacccagacc atgagaatca aggtggtcga gggcggccct ctccccttcg ccttcgacat
     6061 cctggctacc agcttcatgt acggcagcag aaccttcatc aaccacaccc agggcatccc
     6121 cgacttcttt aagcagtcct tccctgaggg cttcacatgg gagagagtca ccacatacga
     6181 agacgggggc gtgctgaccg ctacccagga caccagcctc caggacggct gcctcatcta
     6241 caacgtcaag atcagagggg tgaacttccc atccaacggc cctgtgatgc agaagaaaac
     6301 actcggctgg gaggccaaca ccgagatgct ataccccgct gatggcggcc tggagggacg
     6361 taccgacatg gccctgaagc tcgtgggcgg gggccacctg atctgcaact tcaagaccac
     6421 atacagatcc aagaaacccg ctaagaacct caagatgccc ggcgtctact atgtggacca
     6481 cagactggaa agaatcaagg aggccgacaa agagacctac gtcgagcagc acgaggtggc
     6541 tgtggccaga tactgcgacc tccctagcaa actggggcac aaacttaatg gcatggacga
     6601 gctgtacaag gcatgcgata tcacaagttt gtacaaaaaa gctgaacgag aaacgtaaaa
     6661 tgatataaat atcaatatat taaattagat tttgcataaa aaacagacta cataatactg
     6721 taaaacacaa catatccagt cactatggcg gccgcattag gcaccccagg ctttacactt
     6781 tatgcttccg gctcgtataa tgtgtggatt ttgagttagg atccgtcgag attttcagga
     6841 gctaaggaag ctaaaatgga gaaaaaaatc actggatata ccaccgttga tatatcccaa
     6901 tggcatcgta aagaacattt tgaggcattt cagtcagttg ctcaatgtac ctataaccag
     6961 accgttcagc tggatattac ggccttttta aagaccgtaa agaaaaataa gcacaagttt
     7021 tatccggcct ttattcacat tcttgcccgc ctgatgaatg ctcatccgga attccgtatg
     7081 gcaatgaaag acggtgagct ggtgatatgg gatagtgttc acccttgtta caccgttttc
     7141 catgagcaaa ctgaaacgtt ttcatcgctc tggagtgaat accacgacga tttccggcag
     7201 tttctacaca tatattcgca agatgtggcg tgttacggtg aaaacctggc ctatttccct
     7261 aaagggttta ttgagaatat gtttttcgtc tcagccaatc cctgggtgag tttcaccagt
     7321 tttgatttaa acgtggccaa tatggacaac ttcttcgccc ccgttttcac catgggcaaa
     7381 tattatacgc aaggcgacaa ggtgctgatg ccgctggcga ttcaggttca tcatgccgtt
     7441 tgtgatggct tccatgtcgg cagaatgctt aatgaattac aacagtactg cgatgagtgg
     7501 cagggcgggg cgtaaacgcg tggatccggc ttactaaaag ccagataaca gtatgcgtat
     7561 ttgcgcgctg atttttgcgg tataagaata tatactgata tgtatacccg aagtatgtca
     7621 aaaagaggta tgctatgaag cagcgtatta cagtgacagt tgacagcgac agctatcagt
     7681 tgctcaaggc atatatgatg tcaatatctc cggtctggta agcacaacca tgcagaatga
     7741 agcccgtcgt ctgcgtgccg aacgctggaa agcggaaaat caggaaggga tggctgaggt
     7801 cgcccggttt attgaaatga acggctcttt tgctgacgag aacaggggct ggtgaaatgc
     7861 agtttaaggt ttacacctat aaaagagaga gccgttatcg tctgtttgtg gatgtacaga
     7921 gtgatattat tgacacgccc gggcgacgga tggtgatccc cctggccagt gcacgtctgc
     7981 tgtcagataa agtctcccgt gaactttacc cggtggtgca tatcggggat gaaagctggc
     8041 gcatgatgac caccgatatg gccagtgtgc cggtctccgt tatcggggaa gaagtggctg
     8101 atctcagcca ccgcgaaaat gacatcaaaa acgccattaa cctgatgttc tggggaatat
     8161 aaatgtcagg ctcccttata cacagccagt ctgcaggtcg accatagtga ctggatatgt
     8221 tgtgttttac agtattatgt agtctgtttt ttatgcaaaa tctaatttaa tatattgata
     8281 tttatatcat tttacgtttc tcgttcagct ttcttgtaca aagtggtgat atcgagctct
     8341 cccgggaatt cggatccggc cctcgagggt acctctagag gatctttgtg aaggaacctt
     8401 acttctgtgg tgtgacataa ttggacaaac tacctacaga gatttaaagc tctaaggtaa
     8461 atataaaatt tttaagtgta taatgtgtta aactactgat tctaattgtt tgtgtatttt
     8521 agattccaac ctatggaact gatgaatggg agcagtggtg gaatgccttt aatgaggaaa
     8581 acctgttttg ctcagaagaa atgccatcta gtgatgatga ggctactgct gactctcaac
     8641 attctactcc tccaaaaaag aagagaaagg tagaagaccc caaggacttt ccttcagaat
     8701 tgctaagttt tttgagtcat gctgtgttta gtaatagaac tcttgcttgc tttgctattt
     8761 acaccacaaa ggaaaaagct gcactgctat acaagaaaat tatggaaaaa tatttgatgt
     8821 atagtgcctt gactagagat cataatcagc cataccacat ttgtagaggt tttacttgct
     8881 ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc aattgttgtt
     8941 gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     9001 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     9061 tcttatcatg tctggatctt ggccacgtaa taagtgtgcg ttgaatttat tcgcaaaaac
     9121 attgcatatt ttcggcaaag taaaattttg ttgcatacct tatcaaaaaa taagtgctgc
     9181 atacttttta gagaaaccaa ataatttttt attgcatacc cgtttttaat aaaatacatt
     9241 gcataccctc ttttaataaa aaatattgca tactttgacg aaacaaattt tcgttgcata
     9301 cccaataaaa gattattata ttgcataccc gtttttaata aaatacattg cataccctct
     9361 tttaataaaa aatattgcat acgttgacga aacaaatttt cgttgcatac ccaataaaag
     9421 attattatat tgcatacctt ttcttgccat accatttagc cgatcaattg tgctcggcaa
     9481 cagtatattt gtggtgtgcc aaccaacaac ggatccacta gtgtcgacga tgtaggtcac
     9541 ggtctcgaag ccgcggtgcg ggtgccaggg cgtgcccttg ggctccccgg gcgcgtactc
     9601 cacctcaccc atctggtcca tcatgatgaa cgggtcgagg tggcggtagt tgatcccggc
     9661 gaacgcgcgg cgcaccggga agccctcgcc ctcgaaaccg ctgggcgcgg tggtcacggt
     9721 gagcacggga cgtgcgacgg cgtcggcggg tgcggatacg cggggcagcg tcagcgggtt
     9781 ctcgacggtc acggcgggca tgtcgacact agttctagcc agcttttgtt ccctttagtg
     9841 agggttaatt tcgagcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta
     9901 tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc
     9961 ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg
    10021 aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg
    10081 tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg
    10141 gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa
    10201 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
    10261 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
    10321 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
    10381 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
    10441 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
    10501 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
    10561 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
    10621 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
    10681 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
    10741 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
    10801 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
    10861 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
    10921 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
    10981 atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg
    11041 cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg
    11101 actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc
    11161 aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc
    11221 cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa
    11281 ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc
    11341 cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg
    11401 ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc
    11461 cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat
    11521 ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg
    11581 tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc
    11641 ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg
    11701 aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat
    11761 gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg
    11821 gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg
    11881 ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct
    11941 catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac
    12001 atttccccga aaagtgccac
//
