LOCUS       sequence-42862-e        1337 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1337)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1337)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8
FEATURES             Location/Qualifiers
     source          1..1337
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        541..559
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     541..558
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    695..829
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(719..738)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     773..792
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(942..962)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(942..960)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(981..997)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(981..997)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(994..1016)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1005..1021
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1029..1035,1036..1053,1054..1059))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1074..1095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1212..1229)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 taataccgcc catagccact ccccccattg acgtcaatgg tctctatata tggtctttcc
       61 tattgacgtc atatgggcgg tcctattgac gtatatggcg cctcccccat tgacgtcaat
      121 tacggtaaat ggcccgcctg gctcaatgcc cattgacgtc aataggacca cccacccatt
      181 gacgtcaatg ggatggctca ttgcccattc atatccgttc tcacgccccc tattgacgtc
      241 aatgacggta aatggcccac ttggcagtac atcaatatct attaatagta acttggcaag
      301 tacattacta ttggaagtac gccagggtac attggcagta ctcccattga cgtcaatggc
      361 ggtaaatggc ccgcgatggc tgccaagtac atccccattg acgtcaatgg ggaggggcaa
      421 tgacgcaaat gggcgttcca ttgacgtaaa tgggcggtag gcgtgcctaa tgggaggtct
      481 atataagcaa tgctcgttta gggaaccgcc attctgcctg gggacgtcgg agcaagcttg
      541 atttaggtga cactatagaa tacaagctac ttgttctttt tgcaggatcc actagtggcg
      601 cgccattaat taaaggccgg ccagcgatcg ccggacatcg attggaattc aaggcctctc
      661 gagcctctag aactatagtg agtcgtatta cgtagatcca gacatgataa gatacattga
      721 tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg
      781 tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa
      841 ttgcattcat tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aattcgcggc
      901 cgcggcgcca atgcattggg cccggtaccc agcttttgtt ccctttagtg agggttaatt
      961 gcgcgcttgg cgtaatcatg gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
     1021 attccacaca acatacgagc cgggagcata aagtgtaaag cctggggtgc ctaatgagtg
     1081 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
     1141 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
     1201 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
     1261 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
     1321 aacatgtgag caaaagg
//
