LOCUS       sequence-42863-e        1135 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8CmCherry
FEATURES             Location/Qualifiers
     source          1..1135
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        106..124
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     106..123
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             210..920
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(355..373)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     623..642
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    971..1105
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(995..1014)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1049..1068
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 ttccattgac gtaaatgggc ggtaggcgtg cctaatggga ggtctatata agcaatgctc
       61 gtttagggaa ccgccattct gcctggggac gtcggagcaa gcttgattta ggtgacacta
      121 tagaatacaa gctacttgtt ctttttgcag gatccactag tggcgcgcca ttaattaaag
      181 gccggccagc gatcgccgga catcgattga tggtgagcaa gggcgaggag gataacatgg
      241 ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga gggctccgtg aacggccacg
      301 agttcgagat cgagggcgag ggcgagggcc gcccctacga gggcacccag accgccaagc
      361 tgaaggtgac caagggtggc cccctgccct tcgcctggga catcctgtcc cctcagttca
      421 tgtacggctc caaggcctac gtgaagcacc ccgccgacat ccccgactac ttgaagctgt
      481 ccttccccga gggcttcaag tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga
      541 ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg
      601 gcaccaactt cccctccgac ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct
      661 cctccgagcg gatgtacccc gaggacggcg ccctgaaggg cgagatcaag cagaggctga
      721 agctgaagga cggcggccac tacgacgctg aggtcaagac cacctacaag gccaagaagc
      781 ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt ggacatcacc tcccacaacg
      841 aggactacac catcgtggaa cagtacgaac gcgccgaggg ccgccactcc accggcggca
      901 tggacgagct gtacaagtaa gaattcaagg cctctcgagc ctctagaact atagtgagtc
      961 gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1021 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1081 cattataagc tgcaataaac aagttaacaa caacaattgc attcatttta tgttt
//
