LOCUS       sequence-42864-e        1143 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1143)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NmCherry
FEATURES             Location/Qualifiers
     source          1..1143
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        138..156
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     138..155
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             203..910
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(348..366)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     616..635
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     polyA_signal    1003..1137
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1027..1046)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1081..1100
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 tcaatgggga ggggcaatga cgcaaatggg cgttccattg acgtaaatgg gcggtaggcg
       61 tgcctaatgg gaggtctata taagcaatgc tcgtttaggg aaccgccatt ctgcctgggg
      121 acgtcggagc aagcttgatt taggtgacac tatagaatac aagctacttg ttctttttgc
      181 aggatccact agtcatcgat tgatggtgag caagggcgag gaggataaca tggccatcat
      241 caaggagttc atgcgcttca aggtgcacat ggagggctcc gtgaacggcc acgagttcga
      301 gatcgagggc gagggcgagg gccgccccta cgagggcacc cagaccgcca agctgaaggt
      361 gaccaagggt ggccccctgc ccttcgcctg ggacatcctg tcccctcagt tcatgtacgg
      421 ctccaaggcc tacgtgaagc accccgccga catccccgac tacttgaagc tgtccttccc
      481 cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac ggcggcgtgg tgaccgtgac
      541 ccaggactcc tccctgcagg acggcgagtt catctacaag gtgaagctgc gcggcaccaa
      601 cttcccctcc gacggccccg taatgcagaa gaagaccatg ggctgggagg cctcctccga
      661 gcggatgtac cccgaggacg gcgccctgaa gggcgagatc aagcagaggc tgaagctgaa
      721 ggacggcggc cactacgacg ctgaggtcaa gaccacctac aaggccaaga agcccgtgca
      781 gctgcccggc gcctacaacg tcaacatcaa gttggacatc acctcccaca acgaggacta
      841 caccatcgtg gaacagtacg aacgcgccga gggccgccac tccaccggcg gcatggacga
      901 gctgtacaag gaattcggag ccggcgcgcc attaattaaa ggccggccag cgatcgccaa
      961 ggcctctcga gcctctagaa ctatagtgag tcgtattacg tagatccaga catgataaga
     1021 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt
     1081 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
     1141 aac
//
