LOCUS       sequence-42866-e        1291 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Gokirmak T, Campanale JP, Shipp LE, Moy GW, Tao H, Hamdoun A
  TITLE     Localization and Substrate Selectivity of Sea Urchin Multidrug (MDR)
            Efflux Transporters.
  JOURNAL   J Biol Chem. 2012 Nov 2.
  PUBMED    23124201
REFERENCE   2  (bases 1 to 1291)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2012 Nov 2."
COMMENT     Sequence Label: pCS2+8NCerulean
FEATURES             Location/Qualifiers
     source          1..1291
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        277..295
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     277..294
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(342..344,345..347,348..1058)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(387..408)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(648..667)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     995..1016
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    1151..1285
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1175..1194)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1229..1248
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 cagtacatca atatctatta atagtaactt ggcaagtaca ttactattgg aagtacgcca
       61 gggtacattg gcagtactcc cattgacgtc aatggcggta aatggcccgc gatggctgcc
      121 aagtacatcc ccattgacgt caatggggag gggcaatgac gcaaatgggc gttccattga
      181 cgtaaatggg cggtaggcgt gcctaatggg aggtctatat aagcaatgct cgtttaggga
      241 accgccattc tgcctgggga cgtcggagca agcttgattt aggtgacact atagaataca
      301 agctacttgt tctttttgca ggatccacta gtcatcgatt gatggtgagc aagggcgagg
      361 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
      421 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
      481 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgacct
      541 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
      601 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
      661 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
      721 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca
      781 tcagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
      841 agatccgcca caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
      901 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
      961 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     1021 ccgccgggat cactctcggc atggacgagc tgtacaagga attcggagcc ggcgcgccat
     1081 taattaaagg ccggccagcg atcgccaagg cctctcgagc ctctagaact atagtgagtc
     1141 gtattacgta gatccagaca tgataagata cattgatgag tttggacaaa ccacaactag
     1201 aatgcagtga aaaaaatgct ttatttgtga aatttgtgat gctattgctt tatttgtaac
     1261 cattataagc tgcaataaac aagttaacaa c
//
