LOCUS       sequence-428675-        2414 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive promoter for gene expression in S. pombe. Part type 2 
            following the YeastToolkit MoClo grammar..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
  TITLE     POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic 
            Biology.
  JOURNAL   ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
  PUBMED    37991801
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529."
COMMENT     Sequence Label: pPOM021_P2_pTUB1
FEATURES             Location/Qualifiers
     source          1..2414
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(13..47)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(118..777)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     692..711
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      959..1547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1448..1467
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccgaccagac caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg
       61 agttctgagg tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta
      121 cgccccgccc tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg
      181 gaagccatca caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc
      241 ttgcgtataa tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac
      301 gtttaaatca aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc
      361 aataaaccct ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata
      421 tatgtgtaga aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc
      481 agtttgctca tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc
      541 gtctttcatt gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat
      601 aaaggccgga taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc
      661 cagctgaacg gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc
      721 tttacgatgc cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt
      781 agcttcctta gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat
      841 ttcattatgg tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt
      901 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
      961 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     1021 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     1081 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     1141 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     1201 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     1261 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     1321 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     1381 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     1441 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     1501 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     1561 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     1621 tatcccctga ttctgtggat aaccgtagtc ggtctcaaac gatcctcatt ctttggagcc
     1681 ctttcacttg ttttcaactt atacactgta ctcatttttt ttaaatcgtt ttataaaagc
     1741 ctaataacag tgttccaaat aaatcaaaac tttttccgcg taactctagt ataagccgga
     1801 acaaggctaa gaaacaaata aatgcaacgt ttttatattt cgatctatca aatcctcaat
     1861 tacggaaggg caattagtag cgacacaaga gatatacgct gctgacaagt ttttcgtcgt
     1921 aataaaagag cttttgaaga gaaaaaaatt acggcggagc gttgcaattc gttatatata
     1981 atgtgctatg cataaactct acttttgcta ggaattctcc ttgcccttat gtaatatttt
     2041 atatactttt aaaaagattt acatgaggat atacctgttc tataaaggag cacatgttga
     2101 gttttaatgg aaatttacca atccaatctt tcacttacaa attgagaaaa taaaaggtga
     2161 aattgatgaa gatcctaatc tttttatgat tagaaagctt aaaacacctg actgagtaag
     2221 cttctttttg caagcaatgc attattttaa tgtctattta catgtttagc atacttcctt
     2281 ccgtatcatc aataaatatc gttagacttc tttattaagg aattgttttg attaagtgta
     2341 caccgcactt cactacaaac caaacaccaa aaacctcgta gaaaccctgg aaagacgttc
     2401 agatctatgt gaga
//
