LOCUS       sequence-428679-        2837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Regulatable promoter for gene expression in S. pombe. Part type 2 
            following the YeastToolkit MoClo grammar..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Hebra T, Smrckova H, Elkatmis B, Prevorovsky M, Pluskal T
  TITLE     POMBOX: A Fission Yeast Cloning Toolkit for Molecular and Synthetic 
            Biology.
  JOURNAL   ACS Synth Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529.
  PUBMED    37991801
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2023 Nov 22. doi: 10.1021/acssynbio.3c00529."
COMMENT     Sequence Label: pPOM026_P2_pNMT1
FEATURES             Location/Qualifiers
     source          1..2837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(11..45)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(116..775)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     690..709
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      957..1545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1446..1465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gaccagacca ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag
       61 ttctgaggtc attactggat ctatcaacag gagtccaagc gagctcgata tcaaattacg
      121 ccccgccctg ccactcatcg cagtactgtt gtaattcatt aagcattctg ccgacatgga
      181 agccatcaca aacggcatga tgaacctgaa tcgccagcgg catcagcacc ttgtcgcctt
      241 gcgtataata tttgcccatg gtgaaaacgg gggcgaagaa gttgtccata ttggccacgt
      301 ttaaatcaaa actggtgaaa ctcacccagg gattggctga aacgaaaaac atattctcaa
      361 taaacccttt agggaaatag gccaggtttt caccgtaaca cgccacatct tgcgaatata
      421 tgtgtagaaa ctgccggaaa tcgtcgtggt attcactcca gagcgatgaa aacgtttcag
      481 tttgctcatg gaaaacggtg taacaagggt gaacactatc ccatatcacc agctcaccgt
      541 ctttcattgc catacgaaat tccggatgag cattcatcag gcgggcaaga atgtgaataa
      601 aggccggata aaacttgtgc ttatttttct ttacggtctt taaaaaggcc gtaatatcca
      661 gctgaacggt ctggttatag gtacattgag caactgactg aaatgcctca aaatgttctt
      721 tacgatgcca ttgggatata tcaacggtgg tatatccagt gatttttttc tccattttag
      781 cttccttagc tcctgaaaat ctcgataact caaaaaatac gcccggtagt gatcttattt
      841 cattatggtg aaagttggaa cctcttacgt gcccgatcaa tcatgaccaa aatcccttaa
      901 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     1021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     1081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     1141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1621 tcccctgatt ctgtggataa ccgtagtcgg tcggtctcaa acgtcgccat aaaagacaga
     1681 ataagtcatc agcggttgtt tcatttccta tatttttttt ttattttttt attttttaat
     1741 aagggaaaat ttaacgtcta aggatacaga agattgttag cacattaaag taataaaggc
     1801 ttaagtagta agtgccttag catgttattg tatttcaaag gacataatct aaaataataa
     1861 caatatcatt tctcacaagt tattcaattt tctttttttt ttctaataat atcaagaatg
     1921 tattatttgt ttgacataag tcaactaatt tatttaatat gctggattaa tcttgcagac
     1981 atgtaaatta acaagtttta gtcaaataac gttgaagttt caatgaactc aaataatttc
     2041 tctttttttt tatataacca tatgtctaat ctgatttata ttttccgcag ggatcaactg
     2101 aagttatgac atttggattg gatcacttat aaccttggtc gccaaataat acaaaaatca
     2161 gcgttataaa acaaagaagg tttttgttaa gaaattaatc ctctttcttg ataagaaagt
     2221 tgaaccgaaa ttgcagatac tgatatatga aaataatacc cacaattttg ggaatagcgc
     2281 aagcctcaat ttaaacaata ggtgaggaca catgataatg acctcaatga ttgttagaag
     2341 aaaagagcct cattacaaaa tcgaaaaatg aatggttggg tacaagtttc caaaacatgg
     2401 taaagtggac tttgcgtatg acacgtaaat agaaaaaaac acttgttata tgttttctag
     2461 aattattgtt gtctctttat ggttggatga tgcaaaatag taatttcggt tagttgctgt
     2521 aaaacaccac gagacaaata gatatggata tttattaaat caggaaaaac gtaactctcg
     2581 gctactggat ggttcagtca cccaacgatt actggggaga gaaaacaggg caaaagcaaa
     2641 gcttaaagga atccgattgt cattcggcaa tgtgcagcga aactaaaaac cggataatgg
     2701 acctgttaat cgaaacattg aagatatata aaggaagagg aatcctggca tatcatcaat
     2761 tgaataagtt gaattaatta tttcaatctc attctcactt tctgacttat agtcgctttg
     2821 ttaaaagatc tatgtga
//
