LOCUS       sequence-428697-       12722 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac donor vector containing an 8-kb TetO operator array flanked
            by 3xCTCF sites for random integration using PBase transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12722)
  AUTHORS   Mach P, Kos PI, Zhan Y, Cramard J, Gaudin S, Tunnermann J, Marchi E,
            Eglinger J, Zuin J, Kryzhanovska M, Smallwood S, Gelman L, Roth G, 
            Nora EP, Tiana G, Giorgetti L
  TITLE     Cohesin and CTCF control the dynamics of chromosome folding.
  JOURNAL   Nat Genet. 2022 Dec;54(12):1907-1918. doi: 
            10.1038/s41588-022-01232-7. Epub 2022 Dec 5.
  PUBMED    36471076
REFERENCE   2  (bases 1 to 12722)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-022-01232-7"; journalName: "Nat Genet"; date: 
            "2022-12"; volume: "54"; issue: "12"; pages: "1907-1918"
COMMENT     Sequence Label: PB-3xCTCF-TetO
FEATURES             Location/Qualifiers
     source          1..12722
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1054..1116
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        1320..1338
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     1361..1380
                     /label=LacI-R
                     /note="LacI, reverse primer"
     protein_bind    1402..1435
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    1756..1789
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(1852..1870)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     protein_bind    join(1922..1940,1941..1963,1964..1982,1983..2005,2006..2024,
                     2025..2047,2048..2066,2067..2089,2090..2108,2109..2131,
                     2132..2150,2151..2173,2174..2192)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2269..2287,2288..2310,2311..2329,2330..2352,2353..2371,
                     2372..2394,2395..2413,2414..2436,2437..2455,2456..2478,
                     2479..2497,2498..2520,2521..2539)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2616..2634,2635..2657,2658..2676,2677..2699,2700..2718,
                     2719..2741,2742..2760,2761..2783,2784..2802,2803..2825,
                     2826..2844,2845..2867,2868..2886)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2963..2981,2982..3004,3005..3023,3024..3046,3047..3065,
                     3066..3088,3089..3107,3108..3130,3131..3149,3150..3172,
                     3173..3191,3192..3214,3215..3233)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3310..3328,3329..3351,3352..3370,3371..3393,3394..3412,
                     3413..3435,3436..3454,3455..3477,3478..3496,3497..3519,
                     3520..3538,3539..3561,3562..3580)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    3657..3675
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3699..3717
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3741..3759
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3783..3801
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    join(3878..3896,3897..3919,3920..3938,3939..3961,3962..3980,
                     3981..4003,4004..4022,4023..4045,4046..4064,4065..4087,
                     4088..4106,4107..4129,4130..4148)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4225..4243,4244..4266,4267..4285,4286..4308,4309..4327,
                     4328..4350,4351..4369,4370..4392,4393..4411,4412..4434,
                     4435..4453,4454..4476,4477..4495)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4572..4590,4591..4613,4614..4632,4633..4655,4656..4674,
                     4675..4697,4698..4716,4717..4739,4740..4758,4759..4781,
                     4782..4800,4801..4823,4824..4842)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4919..4937,4938..4960,4961..4979,4980..5002,5003..5021,
                     5022..5044,5045..5063,5064..5086,5087..5105,5106..5128,
                     5129..5147,5148..5170,5171..5189)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5266..5284,5285..5307,5308..5326,5327..5349,5350..5368,
                     5369..5391,5392..5410,5411..5433,5434..5452,5453..5475,
                     5476..5494,5495..5517,5518..5536)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5613..5631,5632..5654,5655..5673,5674..5696,5697..5715,
                     5716..5738,5739..5757,5758..5780,5781..5799,5800..5822,
                     5823..5841,5842..5864,5865..5883)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5960..5978,5979..6001,6002..6020,6021..6043,6044..6062,
                     6063..6085,6086..6104,6105..6127,6128..6146,6147..6169,
                     6170..6188,6189..6211,6212..6230)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6307..6325,6326..6348,6349..6367,6368..6390,6391..6409,
                     6410..6432,6433..6451,6452..6474,6475..6493,6494..6516,
                     6517..6535,6536..6558,6559..6577)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6654..6672,6673..6695,6696..6714,6715..6737,6738..6756,
                     6757..6779,6780..6798,6799..6821,6822..6840,6841..6863,
                     6864..6882,6883..6905,6906..6924)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7001..7019,7020..7042,7043..7061,7062..7084,7085..7103,
                     7104..7126,7127..7145,7146..7168,7169..7187,7188..7210,
                     7211..7229,7230..7252,7253..7271)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7348..7366,7367..7389,7390..7408,7409..7431,7432..7450,
                     7451..7473,7474..7492,7493..7515,7516..7534,7535..7557,
                     7558..7576,7577..7599,7600..7618)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7695..7713,7714..7736,7737..7755,7756..7778,7779..7797,
                     7798..7820,7821..7839,7840..7862,7863..7881,7882..7904,
                     7905..7923,7924..7946,7947..7965)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7737..7755,7756..7778,7779..7797,7798..7820,7821..7839,
                     7840..7862,7863..7881,7882..7904,7905..7923,7924..7946,
                     7947..7965,7966..7988,7989..8007)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7779..7797,7798..7820,7821..7839,7840..7862,7863..7881,
                     7882..7904,7905..7923,7924..7946,7947..7965,7966..7988,
                     7989..8007,8008..8030,8031..8049)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8126..8144,8145..8167,8168..8186,8187..8209,8210..8228,
                     8229..8251,8252..8270,8271..8293,8294..8312,8313..8335,
                     8336..8354,8355..8377,8378..8396)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8473..8491,8492..8514,8515..8533,8534..8556,8557..8575,
                     8576..8598,8599..8617,8618..8640,8641..8659,8660..8682,
                     8683..8701,8702..8724,8725..8743)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8820..8838,8839..8861,8862..8880,8881..8903,8904..8922,
                     8923..8945,8946..8964,8965..8987,8988..9006,9007..9029,
                     9030..9048,9049..9071,9072..9090)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(9167..9185,9186..9208,9209..9227,9228..9250,9251..9269,
                     9270..9292,9293..9311,9312..9334,9335..9353,9354..9376,
                     9377..9395,9396..9418,9419..9437)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(9514..9532,9533..9555,9556..9574,9575..9597,9598..9616,
                     9617..9639,9640..9658,9659..9681,9682..9700,9701..9723,
                     9724..9742,9743..9765,9766..9784)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(9861..9879,9880..9902,9903..9921,9922..9944,9945..9963,
                     9964..9986,9987..10005,10006..10028,10029..10047,
                     10048..10070,10071..10089,10090..10112,10113..10131)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(10208..10226,10227..10249,10250..10268,10269..10291,
                     10292..10310,10311..10333,10334..10352,10353..10375,
                     10376..10394,10395..10417,10418..10436,10437..10459,
                     10460..10478)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     repeat_region   10870..10904
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(10923..10941)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10948..10965)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10948..10964)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      11106..11561
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11193..11212)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11403..11424
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11587..11691
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11692..11760,11761..12552)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11910..11929)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggagctctt taaccctaga aagataatca tattgtgacg
     1081 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
     1141 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1201 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1261 aaatttcttc tataaagtaa caaagcggcc gctctagagt cgttttacaa cgtcgtgact
     1321 aatacgactc actatagggc aacttaatcg ccttgcagca ggcatactct gcgacatcgt
     1381 cgtaatagcg aagaggcccg cataacttcg tataatgtat gctatacgaa gttattggta
     1441 agatcttctg ttggacagcg tggacttggc cagcaggggg cgctggagca ggtcctccac
     1501 cccaaactgc tagttctgtc agccttagag aggggtccag tgcaatgatg cagtggttag
     1561 agaagatgaa caagacggcc agcaggtggc gccagacacc tacacctgca gacactgagt
     1621 ctgtgaagtt gcaggtggag cagaagagat gctcgccggt ggactataaa ttcttatccc
     1681 agctcgacca ccagggggcg ctggggcggg ccgcccacct gttcctgttc caggagattc
     1741 tctgctagtg tgggcataac ttcgtataat gtatgctata cgaagttatt tacgcgccgg
     1801 agaaaaccgc ttcctgaggc cgatactgtc ttgcgcagcc tgaatggcga accgctgagc
     1861 aataactagc tctagaggat ccgtatcacg aggccctttc gtcttcactc gagtttacca
     1921 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     1981 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     2041 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     2101 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2161 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgggctc ggttcccggg
     2221 tcgagatccg tatcacgagg ccctttcgtc ttcactcgag tttaccactc cctatcagtg
     2281 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     2341 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     2401 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     2461 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     2521 tccctatcag tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg agatccgtat
     2581 cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata gagaaaagtg
     2641 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     2701 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     2761 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     2821 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     2881 tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac gaggcccttt
     2941 cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     3001 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     3061 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     3121 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     3181 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     3241 aagtcgggct cggttcccgg gtcgagatcc gtatcacgag gccctttcgt cttcactcga
     3301 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     3361 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     3421 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     3481 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     3541 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgggctcgg
     3601 ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc
     3661 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     3721 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     3781 actccctatc agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt
     3841 atcacgaggc cctttcgtct tcactcgagt ttaccactcc ctatcagtga tagagaaaag
     3901 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     3961 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     4021 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     4081 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     4141 gatagagaaa agtgaaagtc gggctcggtt cccgggtcga gatccgtatc acgaggccct
     4201 ttcgtcttca ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     4261 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     4321 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     4381 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     4441 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     4501 gaaagtcggg ctcggttccc gggtcgagat ccgtatcacg aggccctttc gtcttcactc
     4561 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     4621 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     4681 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     4741 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     4801 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgggctc
     4861 ggttcccggg tcgagatccg tatcacgagg ccctttcgtc ttcactcgag tttaccactc
     4921 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     4981 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     5041 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     5101 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     5161 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg
     5221 agatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct atcagtgata
     5281 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     5341 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     5401 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     5461 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     5521 ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac
     5581 gaggcccttt cgtcttcact cgagtttacc actccctatc agtgatagag aaaagtgaaa
     5641 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     5701 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     5761 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     5821 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     5881 agaaaagtga aagtcgggct cggttcccgg gtcgagatcc gtatcacgag gccctttcgt
     5941 cttcactcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     6001 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     6061 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     6121 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     6181 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     6241 tcgggctcgg ttcccgggtc gagatccgta tcacgaggcc ctttcgtctt cactcgagtt
     6301 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     6361 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     6421 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     6481 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     6541 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc
     6601 ccgggtcgag atccgtatca cgaggccctt tcgtcttcac tcgagtttac cactccctat
     6661 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     6721 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     6781 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     6841 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     6901 accactccct atcagtgata gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc
     6961 cgtatcacga ggccctttcg tcttcactcg agtttaccac tccctatcag tgatagagaa
     7021 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     7081 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     7141 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     7201 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     7261 agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt cgagatccgt atcacgaggc
     7321 cctttcgtct tcactcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     7381 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     7441 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     7501 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     7561 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     7621 agtgaaagtc gggctcggtt cccgggtcga gatccgtatc acgaggccct ttcgtcttca
     7681 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     7741 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     7801 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     7861 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     7921 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     7981 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     8041 tgatagagaa aagtgaaagt cgggctcggt tcccgggtcg agatccgtat cacgaggccc
     8101 tttcgtcttc actcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt
     8161 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     8221 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     8281 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     8341 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     8401 tgaaagtcgg gctcggttcc cgggtcgaga tccgtatcac gaggcccttt cgtcttcact
     8461 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     8521 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     8581 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     8641 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     8701 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgggct
     8761 cggttcccgg gtcgagatcc gtatcacgag gccctttcgt cttcactcga gtttaccact
     8821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     8881 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     8941 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     9001 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     9061 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgggctcgg ttcccgggtc
     9121 gagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc tatcagtgat
     9181 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     9241 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     9301 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     9361 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     9421 cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc ccgggtcgag atccgtatca
     9481 cgaggccctt tcgtcttcac tcgagtttac cactccctat cagtgataga gaaaagtgaa
     9541 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     9601 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     9661 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     9721 cactccctat cagtgataga gaaaagtgaa agtcaagttt accactccct atcagtgata
     9781 gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc cgtatcacga ggccctttcg
     9841 tcttcactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     9901 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     9961 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
    10021 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
    10081 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
    10141 gtcgggctcg gttcccgggt cgagatccgt atcacgaggc cctttcgtct tcactcgagt
    10201 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
    10261 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
    10321 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
    10381 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
    10441 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gggctcggtt
    10501 cccgggtcga gatcccgaag aagtacgtag agaggaaaag gcgatcgcag aaggagaaag
    10561 gctcttcaag ggatggagaa gatctctcga gaattcccat ctataacaag aaaatatata
    10621 tataataagt tatcacgtaa gtagaacatg aaataacaat ataattatcg tatgagttaa
    10681 atcttaaaag tcacgtaaaa gataatcatg cgtcattttg actcacgcgg tcgttatagt
    10741 tcaaaatcag tgacacttac cgcattgaca agcacgcctc acgggagctc caagcggcga
    10801 ctgagatgtc ctaaatgcac agcgacggat tcgcgctatt tagaaagaga gagcaatatt
    10861 tcaagaatgc atgcgtcaat tttacgcaga ctatctttct agggttaagg tacccaattc
    10921 gccctatagt gagtcgtatt acaattcact ggccgtcgtt ttacaacgtc gtgactggga
    10981 aaaccctggc gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg
    11041 taatagcgaa gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga
    11101 atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
    11161 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
    11221 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
    11281 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
    11341 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
    11401 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
    11461 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
    11521 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
    11581 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
    11641 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
    11701 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
    11761 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
    11821 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
    11881 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
    11941 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
    12001 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
    12061 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
    12121 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
    12181 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
    12241 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
    12301 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
    12361 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
    12421 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
    12481 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
    12541 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
    12601 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
    12661 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
    12721 tc
//
