LOCUS       sequence-428698-       12144 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  piggyBac donor vector containing an 8-kb TetO operator array for 
            random integration using PBase transposase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12144)
  AUTHORS   Mach P, Kos PI, Zhan Y, Cramard J, Gaudin S, Tunnermann J, Marchi E,
            Eglinger J, Zuin J, Kryzhanovska M, Smallwood S, Gelman L, Roth G, 
            Nora EP, Tiana G, Giorgetti L
  TITLE     Cohesin and CTCF control the dynamics of chromosome folding.
  JOURNAL   Nat Genet. 2022 Dec;54(12):1907-1918. doi: 
            10.1038/s41588-022-01232-7. Epub 2022 Dec 5.
  PUBMED    36471076
REFERENCE   2  (bases 1 to 12144)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41588-022-01232-7"; journalName: "Nat Genet"; date: 
            "2022-12"; volume: "54"; issue: "12"; pages: "1907-1918"
COMMENT     Sequence Label: PB-empty-DSE-TetO-8kb
FEATURES             Location/Qualifiers
     source          1..12144
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1012..1030
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     repeat_region   1054..1116
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    join(1344..1362,1363..1385,1386..1404,1405..1427,1428..1446,
                     1447..1469,1470..1488,1489..1511,1512..1530,1531..1553,
                     1554..1572,1573..1595,1596..1614)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(1691..1709,1710..1732,1733..1751,1752..1774,1775..1793,
                     1794..1816,1817..1835,1836..1858,1859..1877,1878..1900,
                     1901..1919,1920..1942,1943..1961)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2038..2056,2057..2079,2080..2098,2099..2121,2122..2140,
                     2141..2163,2164..2182,2183..2205,2206..2224,2225..2247,
                     2248..2266,2267..2289,2290..2308)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2385..2403,2404..2426,2427..2445,2446..2468,2469..2487,
                     2488..2510,2511..2529,2530..2552,2553..2571,2572..2594,
                     2595..2613,2614..2636,2637..2655)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(2732..2750,2751..2773,2774..2792,2793..2815,2816..2834,
                     2835..2857,2858..2876,2877..2899,2900..2918,2919..2941,
                     2942..2960,2961..2983,2984..3002)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    3079..3097
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3121..3139
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3163..3181
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3205..3223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    join(3300..3318,3319..3341,3342..3360,3361..3383,3384..3402,
                     3403..3425,3426..3444,3445..3467,3468..3486,3487..3509,
                     3510..3528,3529..3551,3552..3570)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3647..3665,3666..3688,3689..3707,3708..3730,3731..3749,
                     3750..3772,3773..3791,3792..3814,3815..3833,3834..3856,
                     3857..3875,3876..3898,3899..3917)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(3994..4012,4013..4035,4036..4054,4055..4077,4078..4096,
                     4097..4119,4120..4138,4139..4161,4162..4180,4181..4203,
                     4204..4222,4223..4245,4246..4264)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4341..4359,4360..4382,4383..4401,4402..4424,4425..4443,
                     4444..4466,4467..4485,4486..4508,4509..4527,4528..4550,
                     4551..4569,4570..4592,4593..4611)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(4688..4706,4707..4729,4730..4748,4749..4771,4772..4790,
                     4791..4813,4814..4832,4833..4855,4856..4874,4875..4897,
                     4898..4916,4917..4939,4940..4958)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5035..5053,5054..5076,5077..5095,5096..5118,5119..5137,
                     5138..5160,5161..5179,5180..5202,5203..5221,5222..5244,
                     5245..5263,5264..5286,5287..5305)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5382..5400,5401..5423,5424..5442,5443..5465,5466..5484,
                     5485..5507,5508..5526,5527..5549,5550..5568,5569..5591,
                     5592..5610,5611..5633,5634..5652)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(5729..5747,5748..5770,5771..5789,5790..5812,5813..5831,
                     5832..5854,5855..5873,5874..5896,5897..5915,5916..5938,
                     5939..5957,5958..5980,5981..5999)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6076..6094,6095..6117,6118..6136,6137..6159,6160..6178,
                     6179..6201,6202..6220,6221..6243,6244..6262,6263..6285,
                     6286..6304,6305..6327,6328..6346)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6423..6441,6442..6464,6465..6483,6484..6506,6507..6525,
                     6526..6548,6549..6567,6568..6590,6591..6609,6610..6632,
                     6633..6651,6652..6674,6675..6693)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(6770..6788,6789..6811,6812..6830,6831..6853,6854..6872,
                     6873..6895,6896..6914,6915..6937,6938..6956,6957..6979,
                     6980..6998,6999..7021,7022..7040)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7117..7135,7136..7158,7159..7177,7178..7200,7201..7219,
                     7220..7242,7243..7261,7262..7284,7285..7303,7304..7326,
                     7327..7345,7346..7368,7369..7387)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7159..7177,7178..7200,7201..7219,7220..7242,7243..7261,
                     7262..7284,7285..7303,7304..7326,7327..7345,7346..7368,
                     7369..7387,7388..7410,7411..7429)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7201..7219,7220..7242,7243..7261,7262..7284,7285..7303,
                     7304..7326,7327..7345,7346..7368,7369..7387,7388..7410,
                     7411..7429,7430..7452,7453..7471)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7548..7566,7567..7589,7590..7608,7609..7631,7632..7650,
                     7651..7673,7674..7692,7693..7715,7716..7734,7735..7757,
                     7758..7776,7777..7799,7800..7818)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(7895..7913,7914..7936,7937..7955,7956..7978,7979..7997,
                     7998..8020,8021..8039,8040..8062,8063..8081,8082..8104,
                     8105..8123,8124..8146,8147..8165)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8242..8260,8261..8283,8284..8302,8303..8325,8326..8344,
                     8345..8367,8368..8386,8387..8409,8410..8428,8429..8451,
                     8452..8470,8471..8493,8494..8512)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8589..8607,8608..8630,8631..8649,8650..8672,8673..8691,
                     8692..8714,8715..8733,8734..8756,8757..8775,8776..8798,
                     8799..8817,8818..8840,8841..8859)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(8936..8954,8955..8977,8978..8996,8997..9019,9020..9038,
                     9039..9061,9062..9080,9081..9103,9104..9122,9123..9145,
                     9146..9164,9165..9187,9188..9206)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(9283..9301,9302..9324,9325..9343,9344..9366,9367..9385,
                     9386..9408,9409..9427,9428..9450,9451..9469,9470..9492,
                     9493..9511,9512..9534,9535..9553)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     protein_bind    join(9630..9648,9649..9671,9672..9690,9691..9713,9714..9732,
                     9733..9755,9756..9774,9775..9797,9798..9816,9817..9839,
                     9840..9858,9859..9881,9882..9900)
                     /label=tetracycline response element
                     /bound_moiety="tetracycline repressor TetR"
                     /note="contains seven copies of the tetracycline operator 
                     tetO"
     repeat_region   10292..10326
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     primer_bind     complement(10344..10363)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10345..10363)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10370..10387)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10370..10386)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10379..10401)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10528..10983
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10615..10634)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10825..10846
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        11009..11113
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11114..11182,11183..11974)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11332..11351)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctcg aaattaaccc
     1021 tcactaaagg gaacaaaagc tggagctctt taaccctaga aagataatca tattgtgacg
     1081 tacgttaaag ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag
     1141 gtttatttat taatttgaat agatattaag ttttattata tttacactta catactaata
     1201 ataaattcaa caaacaattt atttatgttt atttatttat taaaaaaaaa caaaaactca
     1261 aaatttcttc tataaagtaa caaagcggcc gctctagagg atccgtatca cgaggccctt
     1321 tcgtcttcac tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     1381 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     1441 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     1501 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     1561 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     1621 aaagtcgggc tcggttcccg ggtcgagatc cgtatcacga ggccctttcg tcttcactcg
     1681 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     1741 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     1801 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     1861 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     1921 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgggctcg
     1981 gttcccgggt cgagatccgt atcacgaggc cctttcgtct tcactcgagt ttaccactcc
     2041 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     2101 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     2161 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     2221 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     2281 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gggctcggtt cccgggtcga
     2341 gatccgtatc acgaggccct ttcgtcttca ctcgagttta ccactcccta tcagtgatag
     2401 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     2461 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     2521 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     2581 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     2641 tatcagtgat agagaaaagt gaaagtcggg ctcggttccc gggtcgagat ccgtatcacg
     2701 aggccctttc gtcttcactc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     2761 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     2821 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     2881 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     2941 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     3001 gaaaagtgaa agtcgggctc ggttcccggg tcgagatccg tatcacgagg ccctttcgtc
     3061 ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     3121 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     3181 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgggct
     3241 cggttcccgg gtcgagatcc gtatcacgag gccctttcgt cttcactcga gtttaccact
     3301 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     3361 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     3421 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     3481 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     3541 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgggctcgg ttcccgggtc
     3601 gagatccgta tcacgaggcc ctttcgtctt cactcgagtt taccactccc tatcagtgat
     3661 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     3721 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     3781 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     3841 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     3901 cctatcagtg atagagaaaa gtgaaagtcg ggctcggttc ccgggtcgag atccgtatca
     3961 cgaggccctt tcgtcttcac tcgagtttac cactccctat cagtgataga gaaaagtgaa
     4021 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     4081 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     4141 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     4201 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     4261 gagaaaagtg aaagtcgggc tcggttcccg ggtcgagatc cgtatcacga ggccctttcg
     4321 tcttcactcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
     4381 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     4441 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     4501 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     4561 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     4621 gtcgggctcg gttcccgggt cgagatccgt atcacgaggc cctttcgtct tcactcgagt
     4681 ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt
     4741 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     4801 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     4861 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     4921 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gggctcggtt
     4981 cccgggtcga gatccgtatc acgaggccct ttcgtcttca ctcgagttta ccactcccta
     5041 tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt
     5101 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     5161 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     5221 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     5281 taccactccc tatcagtgat agagaaaagt gaaagtcggg ctcggttccc gggtcgagat
     5341 ccgtatcacg aggccctttc gtcttcactc gagtttacca ctccctatca gtgatagaga
     5401 aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt
     5461 accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga
     5521 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     5581 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     5641 cagtgataga gaaaagtgaa agtcgggctc ggttcccggg tcgagatccg tatcacgagg
     5701 ccctttcgtc ttcactcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
     5761 agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc
     5821 agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga
     5881 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     5941 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     6001 aagtgaaagt cgggctcggt tcccgggtcg agatccgtat cacgaggccc tttcgtcttc
     6061 actcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc
     6121 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa
     6181 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     6241 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     6301 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcgg
     6361 gctcggttcc cgggtcgaga tccgtatcac gaggcccttt cgtcttcact cgagtttacc
     6421 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
     6481 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag
     6541 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     6601 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     6661 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgggct cggttcccgg
     6721 gtcgagatcc gtatcacgag gccctttcgt cttcactcga gtttaccact ccctatcagt
     6781 gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag
     6841 tcgagtttac cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct
     6901 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     6961 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     7021 ctccctatca gtgatagaga aaagtgaaag tcgggctcgg ttcccgggtc gagatccgta
     7081 tcacgaggcc ctttcgtctt cactcgagtt taccactccc tatcagtgat agagaaaagt
     7141 gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac
     7201 tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag
     7261 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     7321 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     7381 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     7441 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgggctcg gttcccgggt
     7501 cgagatccgt atcacgaggc cctttcgtct tcactcgagt ttaccactcc ctatcagtga
     7561 tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     7621 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     7681 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     7741 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     7801 ccctatcagt gatagagaaa agtgaaagtc gggctcggtt cccgggtcga gatccgtatc
     7861 acgaggccct ttcgtcttca ctcgagttta ccactcccta tcagtgatag agaaaagtga
     7921 aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc
     7981 cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa
     8041 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
     8101 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
     8161 agagaaaagt gaaagtcggg ctcggttccc gggtcgagat ccgtatcacg aggccctttc
     8221 gtcttcactc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac
     8281 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata
     8341 gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga
     8401 gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca
     8461 gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga gaaaagtgaa
     8521 agtcgggctc ggttcccggg tcgagatccg tatcacgagg ccctttcgtc ttcactcgag
     8581 tttaccactc cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag
     8641 tgatagagaa aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa
     8701 gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
     8761 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
     8821 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgggctcggt
     8881 tcccgggtcg agatccgtat cacgaggccc tttcgtcttc actcgagttt accactccct
     8941 atcagtgata gagaaaagtg aaagtcgagt ttaccactcc ctatcagtga tagagaaaag
     9001 tgaaagtcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc gagtttacca
     9061 ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat cagtgataga
     9121 gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcaagt
     9181 ttaccactcc ctatcagtga tagagaaaag tgaaagtcgg gctcggttcc cgggtcgaga
     9241 tccgtatcac gaggcccttt cgtcttcact cgagtttacc actccctatc agtgatagag
     9301 aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt
     9361 taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg
     9421 atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt
     9481 cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta
     9541 tcagtgatag agaaaagtga aagtcgggct cggttcccgg gtcgagatcc gtatcacgag
     9601 gccctttcgt cttcactcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
     9661 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
     9721 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
     9781 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
     9841 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
     9901 aaagtgaaag tcgggctcgg ttcccgggtc gagatcccga agaagtacgt agagaggaaa
     9961 aggcgatcgc agaaggagaa aggctcttca agggatggag aagatctctc gagaattccc
    10021 atctataaca agaaaatata tatataataa gttatcacgt aagtagaaca tgaaataaca
    10081 atataattat cgtatgagtt aaatcttaaa agtcacgtaa aagataatca tgcgtcattt
    10141 tgactcacgc ggtcgttata gttcaaaatc agtgacactt accgcattga caagcacgcc
    10201 tcacgggagc tccaagcggc gactgagatg tcctaaatgc acagcgacgg attcgcgcta
    10261 tttagaaaga gagagcaata tttcaagaat gcatgcgtca attttacgca gactatcttt
    10321 ctagggttaa ggtacccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg
    10381 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
    10441 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
    10501 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
    10561 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
    10621 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
    10681 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
    10741 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
    10801 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
    10861 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
    10921 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
    10981 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
    11041 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
    11101 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
    11161 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
    11221 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
    11281 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
    11341 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
    11401 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
    11461 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
    11521 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
    11581 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
    11641 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
    11701 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
    11761 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
    11821 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
    11881 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
    11941 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
    12001 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
    12061 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
    12121 cgtagaaaag atcaaaggat cttc
//
