LOCUS       sequence-428714-       10243 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an 
            inducible lentiviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10243)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: pLVX-TetOne-FFSS-Cyclin D1 (T286A)
FEATURES             Location/Qualifiers
     source          1..10243
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     polyA_signal    2187..2321
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     regulatory      2791..2800
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(3417..3440)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(3477..3500)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(3516..3539)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(3555..3578)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(3591..3958)
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    3716..3734
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3752..3770
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3788..3806
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3824..3842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3860..3878
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3896..3914
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    3932..3950
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        3975..4485
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     4404..4422
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             4504..5250
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        5261..5590
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5441..5576
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5599..6198
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5599..5618)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6095..6115
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6212..6800
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6265..6285)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6683..6694)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7007..7640
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7768..7784)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7768..7784)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7781..7803)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7792..7808
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7816..7822,7823..7840,7841..7846))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7861..7882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7999..8016)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8170..8758)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8250..8269)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8929..9720,9721..9789))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9552..9571
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9790..9894)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    9942..10076
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgacttaact tgtttattgc agcttataat ggttacaaat
     2221 aaggcaatag catcacaaat ttcacaaata aggcattttt ttcactgcat tctagttttg
     2281 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctcaaatccc tcggaagctg
     2341 cgcctgtctt aggttggagt gatacatttt tatcactttt acccgtcttt ggattaggca
     2401 gtagctctga cggccctcct gtcttaggtt agtgaaaaat gtcactctct tacccgtcat
     2461 tggctgtcca gcttagctcg caggggaggt ggtctggatc cgccggcacc ggttcagatg
     2521 tccacgtccc gcacgtcggt gggtgcgcaa gccaggtcca cctcctcctc ctcctcttcc
     2581 tcctcctcgg cggccttggg gtccatgttc tgctgggcct ggcgcaggct tgactccagc
     2641 agggcttcga tctgctcctg gcaggcccgg aggcagtctg ggtcacactt gatcactctg
     2701 gagaggaagc gtgtgaggcg gtagtaggac aggaagttgt tggggctcct caggttcagg
     2761 ccttgcactg cggccaccac gctccccgct gccaccatgg agggcggatt ggaaatgaac
     2821 ttcacatctg tggcacagag ggcaacgaag gtctgcgcgt gtttgcggat gatctgtttg
     2881 ttctcctccg cctctggcat tttggagagg aagtgttcaa tgaaatcgtg cggggtcatt
     2941 gcggccaggt tccacttgag cttgttcacc aggagcagct ccatttgcag cagctcctcg
     3001 ggccggatgg agttgtcggt gtagatgcac agcttctcgg ccgtcagggg gatggtctcc
     3061 ttcatcttag aggccacgaa catgcaagtg gcccccagca gctgcaggcg gctctttttc
     3121 acgggctcca gcgacaggaa gcggtccagg tagttcatgg ccagcgggaa gacctcctcc
     3181 tcgcacttct gttcctcgca gacctccagc atccaggtgg cgacgatctt ccgcatggac
     3241 ggcaggacct ccttctgcac acatttgaag taggacaccg agggcgcgca ggtctcctcc
     3301 gccttcagca tggcccgcag cacccggtcg ttgaggaggt tggcatcggg gtacgcgcgg
     3361 cggatggttt ccacttcgca gcacaggagc tggtgttcgc tgctggatcc agctcctttc
     3421 tcgaactgcg ggtggctcca gcttcctcct ccgctgcctc cgccgcttcc tcctcccttc
     3481 tcgaactgag ggtggctcca gctggcggcc gaccctttat catcatcatc tttataatcc
     3541 ccagatcctg gtaccttgtc atcgtcgtcc ttgtagtcca tggtgaattc tttacgaggg
     3601 taggaagtgg tacggaaagt tggtataaga caaaagtgtt gtggaattga agtttactca
     3661 aaaaatcagc actcttttat aggcgccctg gtttacataa gcaaagctta tacgttctct
     3721 atcactgata gggagtaaac tggatatacg ttctctatca ctgataggga gtaaactgta
     3781 gatacgttct ctatcactga tagggagtaa actggtcata cgttctctat cactgatagg
     3841 gagtaaactc cttatacgtt ctctatcact gatagggagt aaagtctgca tacgttctct
     3901 atcactgata gggagtaaac tcttcatacg ttctctatca ctgataggga gtaaactcga
     3961 ggtgataatt ccacggggtt ggggttgcgc cttttccaag gcagccctgg gtttgcgcag
     4021 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     4081 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     4141 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     4201 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     4261 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     4321 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     4381 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     4441 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccaggggga tcatcgaatt
     4501 accatgtcta gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga
     4561 gtcggtatcg aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct
     4621 accctgtact ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg
     4681 ctggacaggc atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg
     4741 cggaacaacg ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg
     4801 catctcggca cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc
     4861 ctgtgtcagc aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac
     4921 tttacactgg gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag
     4981 acacctacca ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg
     5041 cagggagccg aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa
     5101 cagctaaagt gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg
     5161 ctcccagccg atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac
     5221 gattttgacc ttgacatgct ccccgggtaa acgcgcgaat gtgtgtcagt tagggtgtgg
     5281 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5341 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5401 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5461 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5521 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5581 cttttgcaaa acgcgaccat gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac
     5641 gacgtccccc gggccgtacg caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc
     5701 cacaccgtcg acccggaccg ccacatcgag cgggtcaccg agctgcaaga actcttcctc
     5761 acgcgcgtcg ggctcgacat cggcaaggtg tgggtcgcgg acgacggcgc cgcggtggcg
     5821 gtctggacca cgccggagag cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc
     5881 atggccgagt tgagcggttc ccggctggcc gcgcagcaac agatggaagg cctcctggcg
     5941 ccgcaccggc ccaaggagcc cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac
     6001 cagggcaagg gtctgggcag cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc
     6061 ggggtgcccg ccttcctgga gacctccgcg ccccgcaacc tccccttcta cgagcggctc
     6121 ggcttcaccg tcaccgccga cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc
     6181 cgcaagcccg gtgcctgaac gcgtctggaa caatcaacct ctggattaca aaatttgtga
     6241 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     6301 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     6361 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     6421 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     6481 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     6541 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     6601 ggggaagctg acgtcctttc catggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     6661 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     6721 gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc
     6781 cctttgggcc gcctccccgc ctggaattaa ttctgcagtc gagacctaga aaaacatgga
     6841 gcaatcacaa gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa
     6901 gaggaggagg aggtgggttt tccagtcaca cctcaggtac ctttaagacc aatgacttac
     6961 aaggcagctg tagatcttag ccacttttta aaagaaaaga ggggactgga agggctaatt
     7021 cactcccaac gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc
     7081 cctgattagc agaactacac accagggcca ggggtcagat atccactgac ctttggatgg
     7141 tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac
     7201 accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta
     7261 gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag
     7321 tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca
     7381 gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca
     7441 gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc
     7501 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     7561 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     7621 agtgtggaaa atctctagca gtagtagttc atgtcatctt attattcagt atttataact
     7681 tgcaaagaaa tgaatatcag agagtgagag gccttgacat tgctagcgtt taccgtcgac
     7741 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     7801 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     7861 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     7921 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     7981 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8041 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8101 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8161 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8221 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     8281 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     8341 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     8401 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     8461 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     8521 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     8581 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     8641 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     8701 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     8761 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     8821 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     8881 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     8941 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     9001 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     9061 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     9121 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     9181 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     9241 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     9301 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     9361 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     9421 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     9481 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     9541 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     9601 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     9661 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     9721 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     9781 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     9841 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     9901 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat caacttgttt attgcagctt
     9961 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
    10021 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggatcaact
    10081 ggataactca agctaaccaa aatcatccca aacttcccac cccataccct attaccactg
    10141 ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta ttttcatttt
    10201 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
