LOCUS       sequence-428716-        9855 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses AcGFP-MLH1 in mammalian cells from a retroviral vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9855)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 9855)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: AcGFP-MLH1
FEATURES             Location/Qualifiers
     source          1..9855
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2910
                     /codon_start=1
                     /product="Aequorea coerulescens GFP"
                     /label=AcGFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGAELFTGIVPILIELNGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSYGVQCFSRYPDHMKQHDFFKSAMPEGYIQERTIFFEDD
                     GNYKSRAEVKFEGDTLVNRIELTGTDFKEDGNILGNKMEYNYNAHNVYIMTDKAKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMIYF
                     GFVTAAAITHGMDELYK"
     promoter        5250..5749
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(5285..5308)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     5682..5701
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             5770..6369
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(5770..5789)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6266..6286
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             6554..6986
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7089..7111)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7247..7267)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7252..7581
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7432..7567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7603..7622
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(7703..7720)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7874..8462)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7954..7973)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8633..9424,9425..9493))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9256..9275
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9494..9598)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9666..9684
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(9784..9855,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcgc cgagctgttc
     2221 accggcatcg tgcccatcct gatcgagctg aatggcgatg tgaatggcca caagttcagc
     2281 gtgagcggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
     2341 accaccggca agctgcctgt gccctggccc accctggtga ccaccctgag ctacggcgtg
     2401 cagtgcttct cacgctaccc cgatcacatg aagcagcacg acttcttcaa gagcgccatg
     2461 cctgagggct acatccagga gcgcaccatc ttcttcgagg atgacggcaa ctacaagtcg
     2521 cgcgccgagg tgaagttcga gggcgatacc ctggtgaatc gcatcgagct gaccggcacc
     2581 gatttcaagg aggatggcaa catcctgggc aataagatgg agtacaacta caacgcccac
     2641 aatgtgtaca tcatgaccga caaggccaag aatggcatca aggtgaactt caagatccgc
     2701 cacaacatcg aggatggcag cgtgcagctg gccgaccact accagcagaa tacccccatc
     2761 ggcgatggcc ctgtgctgct gcccgataac cactacctgt ccacccagag cgccctgtcc
     2821 aaggacccca acgagaagcg cgatcacatg atctacttcg gcttcgtgac cgccgccgcc
     2881 atcacccacg gcatggatga gctgtacaag tccggactca gatctcgagg aggcggagga
     2941 agctcgttcg tggcaggggt tattcggcgg ctggacgaga cagtggtgaa ccgcatcgcg
     3001 gcgggggaag ttatccagcg gccagctaat gctatcaaag agatgattga gaactgttta
     3061 gatgcaaaat ccacaagtat tcaagtgatt gttaaagagg gaggcctgaa gttgattcag
     3121 atccaagaca atggcaccgg gatcaggaaa gaagatctgg atattgtatg tgaaaggttc
     3181 actactagta aactgcagtc ctttgaggat ttagccagta tttctaccta tggctttcga
     3241 ggtgaggctt tggccagcat aagccatgtg gctcatgtta ctattacaac gaaaacagct
     3301 gatggaaagt gtgcatacag agcaagttac tcagatggaa aactgaaagc ccctcctaaa
     3361 ccatgtgctg gcaatcaagg gacccagatc acggtggagg acctttttta caacatagcc
     3421 acgaggagaa aagctttaaa aaatccaagt gaagaatatg ggaaaatttt ggaagttgtt
     3481 ggcaggtatt cagtacacaa tgcaggcatt agtttctcag ttaaaaaaca aggagagaca
     3541 gtagctgatg ttaggacact acccaatgcc tcaaccgtgg acaatattcg ctccatcttt
     3601 ggaaatgctg ttagtcgaga actgatagaa attggatgtg aggataaaac cctagccttc
     3661 aaaatgagtg gttacatatc caatgcaaac tactcagtga agaagtgcat cttcttactc
     3721 ttcatcaacc atcgtctggt agaatcaact tccttgagaa aagccataga aacagtgtat
     3781 gcagcctatt tgcccaaaaa cacacaccca ttcctgtacc tcagtttaga aatcagtccc
     3841 cagaatgtgg atgttaatgt gcaccccaca aagcatgaag ttcacttcct gcacgaggag
     3901 agcatcctgg agcgggtgca gcagcacatc gagagcaagc tcctgggctc caattcctcc
     3961 aggatgtact tcacccagac tttgctacca ggacttgctg gcccctctgg ggagatggtt
     4021 aaatccacaa caagtctgac ctcgtcttct acttctggaa gtagtgataa ggtctatgcc
     4081 caccagatgg ttcgtacaga ttcccgggaa cagaagcttg atgcatttct gcagcctctg
     4141 agcaaacccc tgtccagtca gccccaggcc attgtcacag aggataagac agatatttct
     4201 agtggcaggg ctaggcagca agatgaggag atgcttgaac tcccagcccc tgctgaagtg
     4261 gctgccaaaa atcagagctt ggagggggat acaacaaagg ggacttcaga aatgtcagag
     4321 aagagaggac ctacttccag caaccccaga aagagacatc gggaagattc tgatgtggaa
     4381 atggtggaag atgattcccg aaaggaaatg actgcagctt gtaccccccg gagaaggatc
     4441 attaacctca ctagtgtttt gagtctccag gaagaaatta atgagcaggg acatgaggtt
     4501 ctccgggaga tgttgcataa ccactccttc gtgggctgtg tgaatcctca gtgggccttg
     4561 gcacagcatc aaaccaagtt ataccttctc aacaccacca agcttagtga agaactgttc
     4621 taccagatac tcatttatga ttttgccaat tttggtgttc tcaggttatc ggagccagca
     4681 ccgctctttg accttgccat gcttgcctta gatagtccag agagtggctg gacagaggaa
     4741 gatggtccca aagaaggact tgctgaatac attgttgagt ttctgaagaa gaaggctgag
     4801 atgcttgcag actatttctc tttggaaatt gatgaggaag ggaacctgat tggattaccc
     4861 cttctgattg acaactatgt gccccctttg gagggactgc ctatcttcat tcttcgacta
     4921 gccactgagg tgaattggga cgaagaaaag gaatgttttg aaagcctcag taaagaatgc
     4981 gctatgttct attccatccg gaagcagtac atatctgagg agtcgaccct ctcaggccag
     5041 cagagtgaag tgcctggctc cattccaaac tcctggaagt ggactgtgga acacattgtc
     5101 tataaagcct tgcgctcaca cattctgcct cctaaacatt tcacagaaga tggaaatatc
     5161 ctgcagcttg ctaacctgcc tgatctatac aaagtctttg agaggtgcta aggatccacc
     5221 ggatctagat aactgatcat aattctaccg ggtaggggag gcgcttttcc caaggcagtc
     5281 tggagcatgc gctttagcag ccccgctggg cacttggcgc tacacaagtg gcctctggcc
     5341 tcgcacacat tccacatcca ccggtaggcg ccaaccggct ccgttctttg gtggcccctt
     5401 cgcgccacct tctactcctc ccctagtcag gaagttcccc cccgccccgc agctcgcgtc
     5461 gtgcaggacg tgacaaatgg aagtagcacg tctcactagt ctcgtgcaga tggacagcac
     5521 cgctgagcaa tggaagcggg taggcctttg gggcagcggc caatagcagc tttgctcctt
     5581 cgctttctgg gctcagaggc tgggaagggg tgggtccggg ggcgggctca ggggcgggct
     5641 caggggcggg gcgggcgccc gaaggtcctc cggaggcccg gcattctgca cgcttcaaaa
     5701 gcgcacgtct gccgcgctgt tctcctcttc ctcatctccg ggcctttcga cctgcagccc
     5761 aagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga cgacgtcccc
     5821 agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg ccacaccgtc
     5881 gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct cacgcgcgtc
     5941 gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc ggtctggacc
     6001 acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg catggccgag
     6061 ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc gccgcaccgg
     6121 cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca ccagggcaag
     6181 ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc cggggtgccc
     6241 gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct cggcttcacc
     6301 gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac ccgcaagccc
     6361 ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc acgaccccat
     6421 gcatcggcgt ctcgatcgag atatcagtgg tccaggctct agttttgact caacaatatc
     6481 accagctgaa gcctatagag tacgagccat agataaaata aaagatttta tttagtctcc
     6541 agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg caagctacta gcttaagtaa
     6601 cgccattttg caaggcatgg aaaaatacat aactgagaat agagaagttc agatcaaggt
     6661 caggaacaga tggaacaggg tcgatcgacc ctagagaacc atcagatgtt tccagggtgc
     6721 cccaaggacc tgaaatgacc ctgtgcctta tttgaactaa ccaatcagtt cgcttctcgc
     6781 ttctgttcgc gcgcttctgc tccccgagct caataaaaga gcccacaacc cctcactcgg
     6841 ggcgccagtc ctccgattga ctgagtcgcc cgggtacccg tgtatccaat aaaccctctt
     6901 gcagttgcat ccgacttgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga
     6961 ctacccgtca gcgggggtct ttcatttggg ggctcgtccg ggatcgggag acccctgccc
     7021 agggaccacc gacccaccac cgggaggtaa gctggctgcc tcgcgcgttt cggtgatgac
     7081 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat
     7141 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca
     7201 gccatgaccc agtcacgtag cgatagcgga gtgtagatcc ggctgtggaa tgtgtgtcag
     7261 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     7321 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
     7381 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
     7441 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     7501 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     7561 ggaggcctag gcttttgcaa aaagcttact ggcttaacta tgcggcatca gagcagattg
     7621 tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc
     7681 gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     7741 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     7801 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7861 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7921 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7981 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     8041 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     8101 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8161 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8221 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8281 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     8341 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8401 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8461 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8521 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8581 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8641 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8701 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8761 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8821 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8881 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8941 ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     9001 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     9061 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9121 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9181 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9241 cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9301 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9361 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9421 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9481 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9541 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9601 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     9661 ataaaaatag gcgtatcacg aggccctttc gtcttcaaga attagcttgg ccattgcata
     9721 cgttgtatcc atatcataat atgtacattt atattggctc atgtccaaca ttaccgccat
     9781 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
     9841 gcccatatat ggagt
//
