LOCUS       sequence-428745-       10899 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry-POLD1 in mammalian cells from a retroviral 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10899)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: mCherry-POLD1
FEATURES             Location/Qualifiers
     source          1..10899
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2901
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2339..2357)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2607..2626
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(3727..3738)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     promoter        6294..6793
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6329..6352)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6726..6745
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6814..7413
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6814..6833)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7310..7330
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7598..8030
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(8133..8155)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8291..8311)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8296..8625
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8476..8611
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8647..8666
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8747..8764)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8918..9506)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8998..9017)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9677..10468,10469..10537))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10300..10319
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10538..10642)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10710..10728
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10828..10899,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
     2221 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2281 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2341 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2401 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2461 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2521 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2581 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2641 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2701 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2761 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2821 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2881 ggcatggacg agctgtacaa gtccggactc agatctcgag gaggcggagg aagcgatggc
     2941 aagcggcggc caggcccagg gcccggggtg cccccaaagc gggcccgtgg gggcctctgg
     3001 gatgatgatg atgcacctcg gccatcccaa ttcgaggagg acctggcact gatggaggag
     3061 atggaggcag aacacaggct gcaggagcag gaggaggagg agctgcagtc agtcctggag
     3121 ggggttgcag acgggcaggt cccaccatca gccatagatc ctcgctggct tcggcccaca
     3181 ccaccagcgc tggaccccca gacagagccc ctcatcttcc aacagttgga gattgaccat
     3241 tatgtgggcc cagcacagcc tgtgcctggg gggcccccac catcccacgg ctccgtgcct
     3301 gtgctccgcg ccttcggggt caccgatgag gggttctctg tctgctgcca catccacggc
     3361 ttcgctccct acttctacac cccagcgccc cctggtttcg ggcccgagca catgggtgac
     3421 ctgcaacggg agctgaactt ggccatcagc cgggacagtc gcggggggag ggagctgact
     3481 gggccggccg tgctggctgt ggaactgtgc tcccgagaga gcatgtttgg gtaccacggg
     3541 cacggcccct ccccgttcct gcgcatcacc gtggcgctgc cgcgcctcgt ggccccggcc
     3601 cgccgtctcc tggaacaggg catccgtgtg gcaggcctgg gcacgcccag cttcgcgccc
     3661 tacgaggcca acgtcgactt tgagatccgg ttcatggtgg acacggacat cgtcggctgc
     3721 aactggctgg agctcccagc tgggaaatac gccctgaggc tgaaggagaa ggctacgcag
     3781 tgccagctgg aggcggacgt gctgtggtct gacgtggtca gtcacccacc ggaagggcca
     3841 tggcagcgca ttgcgccctt gcgcgtgctc agcttcgata tcgagtgcgc cggccgcaaa
     3901 ggcatcttcc ctgagcctga gcgggaccct gtcatccaga tctgctcgct gggcctgcgc
     3961 tggggggagc cggagccctt cctacgcctg gcgctcaccc tgcggccctg tgcccccatc
     4021 ctgggtgcca aggtgcagag ctacgagaag gaggaggacc tgctgcaggc ctggtccacc
     4081 ttcatccgta tcatggaccc cgacgtgatc accggttaca acatccagaa cttcgacctt
     4141 ccgtacctca tctctcgggc ccagaccctc aaggtacaaa cattcccttt cctgggccgt
     4201 gtggccggcc tttgctccaa catccgggac tcttcattcc agtccaagca gacgggccgg
     4261 cgggacacca aggttgtcag catggtgggc cgcgtgcaga tggacatgct gcaggtgctg
     4321 ctgcgggagt acaagctccg ctcctacacg ctcaatgccg tgagcttcca cttcctgggc
     4381 gagcagaagg aggacgtgca gcacagcatc atcaccgacc tgcagaatgg gaacgaccag
     4441 acccgccgcc gcctggctgt gtactgcctg aaggatgcct acctgccact gcggctgctg
     4501 gagcggctca tggtgctggt gaacgccgtg gagatggcga gggtcactgg cgtgcccctc
     4561 agctacctgc tcagtcgtgg ccagcaggtc aaggtcgtat cccagctgtt gcggcaggcc
     4621 atgcacgagg ggctgctgat gcccgtggtg aagtcagagg gcggcgagga ctacacggga
     4681 gccactgtca tcgagcccct caaagggtac tacgacgtcc ccatcgccac cctggacttc
     4741 tcctcgctgt acccgtccat catgatggcc cacaacctgt gttacaccac gctccttcgg
     4801 cccgggactg cacagaaact gggcctgact gaggatcagt tcatcaggac ccccaccggg
     4861 gacgagtttg tgaagacctc agtgcggaag gggctgctgc cccagatcct ggagaacctg
     4921 ctcagtgccc ggaagagggc caaggccgag ctggccaagg agacagaccc cctccggcgc
     4981 caggtcctgg atggacggca gctggcgctg aaggtgagcg ccaactccgt atacggcttc
     5041 actggcgccc aggtgggcaa gttgccgtgc ctggagatct cacagagcgt cacggggttc
     5101 ggacgtcaga tgatcgagaa aaccaagcag ctggtggagt ctaagtacac agtggagaat
     5161 ggctacagca ccagtgccaa ggtggtgtat ggtgacactg actccgtcat gtgccgattc
     5221 ggcgtgtcct cggtggctga ggcgatggcc ctggggcggg aggccgcgga ctgggtgtca
     5281 ggtcacttcc cgtcgcccat ccggctggag tttgagaagg tctacttccc atacctgctt
     5341 atcagcaaga agcgctacgc gggcctgctc ttctcctccc ggcccgacgc ccacgaccgc
     5401 atggactgca agggcctgga ggccgtgcgc agggacaact gccccctcgt ggccaacctg
     5461 gtcactgcct cactgcgccg cctgctcatc gaccgagacc ctgagggcgc ggtggctcac
     5521 gcacaggacg tcatctcgga cctgctgtgc aaccgcatcg atatctccca gctggtcatc
     5581 accaaggagc tgacccgcgc ggcctccgac tatgccggca agcaggccca cgtggagctg
     5641 gccgagagga tgaggaagcg ggaccccggg agtgcgccca gcctgggcga ccgcgtcccc
     5701 tacgtgatca tcagtgccgc caagggtgtg gccgcctaca tgaagtcgga ggacccgctg
     5761 ttcgtgctgg agcacagcct gcccattgac acgcagtact acctggagca gcagctggcc
     5821 aagcccctcc tgcgcatctt cgagcccatc ctgggcgagg gccgtgccga ggctgtgcta
     5881 ctgcgggggg accacacgcg ctgcaagacg gtgctcacgg gcaaggtggg cggcctcctg
     5941 gccttcgcca aacgccgcaa ctgctgcatt ggctgccgca cagtgctcag ccaccaggga
     6001 gccgtgtgtg agttctgcca gccccgggag tctgagctgt atcagaagga ggtatcccat
     6061 ctgaatgccc tggaggagcg cttctcgcgc ctctggacgc agtgccagcg ctgccagggc
     6121 agcctgcacg aggacgtcat ctgcaccagc cgggactgcc ccatcttcta catgcgcaag
     6181 aaggtgcgga aggacctgga agaccaggag cagctcctgc ggcgcttcgg accccctgga
     6241 cctgaggcct ggtgaggatc caccggatct agataactga tcataattct accgggtagg
     6301 ggaggcgctt ttcccaaggc agtctggagc atgcgcttta gcagccccgc tgggcacttg
     6361 gcgctacaca agtggcctct ggcctcgcac acattccaca tccaccggta ggcgccaacc
     6421 ggctccgttc tttggtggcc ccttcgcgcc accttctact cctcccctag tcaggaagtt
     6481 cccccccgcc ccgcagctcg cgtcgtgcag gacgtgacaa atggaagtag cacgtctcac
     6541 tagtctcgtg cagatggaca gcaccgctga gcaatggaag cgggtaggcc tttggggcag
     6601 cggccaatag cagctttgct ccttcgcttt ctgggctcag aggctgggaa ggggtgggtc
     6661 cgggggcggg ctcaggggcg ggctcagggg cggggcgggc gcccgaaggt cctccggagg
     6721 cccggcattc tgcacgcttc aaaagcgcac gtctgccgcg ctgttctcct cttcctcatc
     6781 tccgggcctt tcgacctgca gcccaagctt accatgaccg agtacaagcc cacggtgcgc
     6841 ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac
     6901 taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt caccgagctg
     6961 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac
     7021 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc
     7081 gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg
     7141 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc
     7201 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag
     7261 gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc
     7321 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc
     7381 acctggtgca tgacccgcaa gcccggtgcc tgacgcccgc cccacgaccc gcagcgcccg
     7441 accgaaagga gcgcacgacc ccatgcatcg gcgtctcgat cgagatatca gtggtccagg
     7501 ctctagtttt gactcaacaa tatcaccagc tgaagcctat agagtacgag ccatagataa
     7561 aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc acctgtaggt
     7621 ttggcaagct actagcttaa gtaacgccat tttgcaaggc atggaaaaat acataactga
     7681 gaatagagaa gttcagatca aggtcaggaa cagatggaac agggtcgatc gaccctagag
     7741 aaccatcaga tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa
     7801 ctaaccaatc agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa
     7861 aagagcccac aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta
     7921 cccgtgtatc caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg
     7981 ggagggtctc ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg
     8041 tccgggatcg ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc
     8101 tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     8161 gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     8221 ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtag
     8281 atccggctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     8341 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     8401 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     8461 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     8521 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     8581 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct tactggctta
     8641 actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt gaaataccgc
     8701 acagatgcgt aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact
     8761 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8821 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8881 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8941 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     9001 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9061 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9121 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9181 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9241 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9301 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9361 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9421 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9481 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9541 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9601 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9661 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9721 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9781 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9841 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9901 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9961 ttaatagttt gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt
    10021 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10081 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10141 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10201 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10261 tgcggcgacc gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca
    10321 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10381 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10441 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10501 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10561 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10621 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa
    10681 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc
    10741 aagaattagc ttggccattg catacgttgt atccatatca taatatgtac atttatattg
    10801 gctcatgtcc aacattaccg ccatgttgac attgattatt gactagttat taatagtaat
    10861 caattacggg gtcattagtt catagcccat atatggagt
//
