LOCUS       sequence-428752-       10623 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses mCherry-PARP1 in mammalian cells from a retroviral 
            vector..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10623)
  AUTHORS   Rona G, Miwatani-Minter B, Zhang Q, Goldberg HV, Kerzhnerman MA, 
            Howard JB, Simoneschi D, Lane E, Hobbs JW, Sassani E, Wang AA, 
            Keegan S, Laverty DJ, Piett CG, Pongor LS, Xu ML, Andrade J, Thomas 
            A, Sicinski P, Askenazi M, Ueberheide B, Fenyo D, Nagel ZD, Pagano M
  TITLE     CDK-independent role of D-type cyclins in regulating DNA mismatch 
            repair.
  JOURNAL   Mol Cell. 2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010.
  PUBMED    38458201
REFERENCE   2  (bases 1 to 10623)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell. 
            2024 Feb 29:S1097-2765(24)00129-1. doi: 
            10.1016/j.molcel.2024.02.010."
COMMENT     Sequence Label: mCherry-PARP1
FEATURES             Location/Qualifiers
     source          1..10623
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        309..512
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             513..688
                     /label=5' LTR (truncated)
                     /note="truncated long terminal repeat from Moloney murine 
                     sarcoma virus"
     misc_feature    751..950
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1151..1567
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1497..1519
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1535..1551
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     enhancer        1642..1945
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1946..2149
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     regulatory      2188..2197
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2194..2901
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(2339..2357)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     2607..2626
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     promoter        6018..6517
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6053..6076)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     6450..6469
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             6538..7137
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6538..6557)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7034..7054
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     LTR             7322..7754
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     Moloney murine leukemia virus"
     primer_bind     complement(7857..7879)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8015..8035)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8020..8349
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8200..8335
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8371..8390
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8471..8488)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8642..9230)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8722..8741)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9401..10192,10193..10261))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10024..10043
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10262..10366)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10434..10452
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     enhancer        join(10552..10623,1..308)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
       61 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      121 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      181 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      241 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      301 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      361 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      421 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      481 gtgtacggtg ggaggtctat ataagcagag ctcaataaaa gagcccacaa cccctcactc
      541 ggcgcgccag tcttccgata gactgcgtcg cccgggtacc cgtattccca ataaagcctc
      601 ttgctgtttg catccgaatc gtggtctcgc tgttccttgg gagggtctcc tctgagtgat
      661 tgactaccca cgacgggggt ctttcatttg ggggctcgtc cgggatttgg agacccctgc
      721 ccagggacca ccgacccacc accgggaggt aagctggcca gcaacttatc tgtgtctgtc
      781 cgattgtcta gtgtctatgt ttgatgttat gcgcctgcgt ctgtactagt tagctaacta
      841 gctctgtatc tggcggaccc gtggtggaac tgacgagttc tgaacacccg gccgcaaccc
      901 tgggagacgt cccagggact ttgggggccg tttttgtggc ccgacctgag gaagggagtc
      961 gatgtggaat ccgaccccgt caggatatgt ggttctggta ggagacgaga acctaaaaca
     1021 gttcccgcct ccgtctgaat ttttgctttc ggtttggaac cgaagccgcg cgtcttgtct
     1081 gctgcagcgc tgcagcatcg ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc
     1141 tgaaaattag ggccagactg ttaccactcc cttaagtttg accttaggtc actggaaaga
     1201 tgtcgagcgg atcgctcaca accagtcggt agatgtcaag aagagacgtt gggttacctt
     1261 ctgctctgca gaatggccaa cctttaacgt cggatggccg cgagacggca cctttaaccg
     1321 agacctcatc acccaggtta agatcaaggt cttttcacct ggcccgcatg gacacccaga
     1381 ccaggtcccc tacatcgtga cctgggaagc cttggctttt gacccccctc cctgggtcaa
     1441 gccctttgta caccctaagc ctccgcctcc tcttcctcca tccgccccgt ctctccccct
     1501 tgaacctcct cgttcgaccc cgcctcgatc ctccctttat ccagccctca ctccttctct
     1561 aggcgccgga attgaagatc atagttatta atagtaatca attacggggt cattagttca
     1621 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
     1681 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
     1741 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
     1801 acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg gtaaatggcc
     1861 cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc agtacatcta
     1921 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca atgggcgtgg
     1981 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
     2041 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg ccccattgac
     2101 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctg gtttagtgaa
     2161 ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga ggaggataac
     2221 atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc cgtgaacggc
     2281 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
     2341 aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct gtcccctcag
     2401 ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga ctacttgaag
     2461 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
     2521 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
     2581 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
     2641 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
     2701 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
     2761 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
     2821 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
     2881 ggcatggacg agctgtacaa gtccggactc agatctgcgg agtcttcgga taagctctat
     2941 cgagtcgagt acgccaagag cgggcgcgcc tcttgcaaga aatgcagcga gagcatcccc
     3001 aaggactcgc tccggatggc catcatggtg cagtcgccca tgtttgatgg aaaagtccca
     3061 cactggtacc acttctcctg cttctggaag gtgggccact ccatccggca ccctgacgtt
     3121 gaggtggatg ggttctctga gcttcggtgg gatgatcagc agaaagtcaa gaagacagcg
     3181 gaagctggag gagtgacagg caaaggccag gatggaattg gtagcaaggc agagaagact
     3241 ctgggtgact ttgcagcaga gtatgccaag tccaacagaa gtacgtgcaa ggggtgtatg
     3301 gagaagatag aaaagggcca ggtgcgcctg tccaagaaga tggtggaccc ggagaagcca
     3361 cagctaggca tgattgaccg ctggtaccat ccaggctgct ttgtcaagaa cagggaggag
     3421 ctgggtttcc ggcccgagta cagtgcgagt cagctcaagg gcttcagcct ccttgctaca
     3481 gaggataaag aagccctgaa gaagcagctc ccaggagtca agagtgaagg aaagagaaaa
     3541 ggcgatgagg tggatggagt ggatgaagtg gcgaagaaga aatctaaaaa agaaaaagac
     3601 aaggatagta agcttgaaaa agccctaaag gctcagaacg acctgatctg gaacatcaag
     3661 gacgagctaa agaaagtgtg ttcaactaat gacctgaagg agctactcat cttcaacaag
     3721 cagcaagtgc cttctgggga gtcggcgatc ttggaccgag tagccgatgg catggtgttc
     3781 ggtgccctcc ttccctgcga ggaatgctcg ggtcagctgg tcttcaagag cgatgcctat
     3841 tactgcactg gggacgtcac tgcctggacc aagtgtatgg tcaagacaca gacacccaac
     3901 cggaaggagt gggtaacccc aaaggaattc cgagaaatct cttacctcaa gaaattgaag
     3961 gttaaaaaac aggaccgtat attcccccca gaaaccagcg cctccgtggc ggccacgcct
     4021 ccgccctcca cagcctcggc tcctgctgct gtgaactcct ctgcttcagc agataagcca
     4081 ttatccaaca tgaagatcct gactctcggg aagctgtccc ggaacaagga tgaagtgaag
     4141 gccatgattg agaaactcgg ggggaagttg acggggacgg ccaacaaggc ttccctgtgc
     4201 atcagcacca aaaaggaggt ggaaaagatg aataagaaga tggaggaagt aaaggaagcc
     4261 aacatccgag ttgtgtctga ggacttcctc caggacgtct ccgcctccac caagagcctt
     4321 caggagttgt tcttagcgca catcttgtcc ccttgggggg cagaggtgaa ggcagagcct
     4381 gttgaagttg tggccccaag agggaagtca ggggctgcgc tctccaaaaa aagcaagggc
     4441 caggtcaagg aggaaggtat caacaaatct gaaaagagaa tgaaattaac tcttaaagga
     4501 ggagcagctg tggatcctga ttctggactg gaacactctg cgcatgtcct ggagaaaggt
     4561 gggaaggtct tcagtgccac ccttggcctg gtggacatcg ttaaaggaac caactcctac
     4621 tacaagctgc agcttctgga ggacgacaag gaaaacaggt attggatatt caggtcctgg
     4681 ggccgtgtgg gtacggtgat cggtagcaac aaactggaac agatgccgtc caaggaggat
     4741 gccattgagc acttcatgaa attatatgaa gaaaaaaccg ggaacgcttg gcactccaaa
     4801 aatttcacga agtatcccaa aaagttctac cccctggaga ttgactatgg ccaggatgaa
     4861 gaggcagtga agaagctgac agtaaatcct ggcaccaagt ccaagctccc caagccagtt
     4921 caggacctca tcaagatgat ctttgatgtg gaaagtatga agaaagccat ggtggagtat
     4981 gagatcgacc ttcagaagat gcccttgggg aagctgagca aaaggcagat ccaggccgca
     5041 tactccatcc tcagtgaggt ccagcaggcg gtgtctcagg gcagcagcga ctctcagatc
     5101 ctggatctct caaatcgctt ttacaccctg atcccccacg actttgggat gaagaagcct
     5161 ccgctcctga acaatgcaga cagtgtgcag gccaaggcgg aaatgcttga caacctgctg
     5221 gacatcgagg tggcctacag tctgctcagg ggagggtctg atgatagcag caaggatccc
     5281 atcgatgtca actatgagaa gctcaaaact gacattaagg tggttgacag agattctgaa
     5341 gaagccgaga tcatcaggaa gtatgttaag aacactcatg caaccacaca caatgcgtat
     5401 gacttggaag tcatcgatat ctttaagata gagcgtgaag gcgaatgcca gcgttacaag
     5461 ccctttaagc agcttcataa ccgaagattg ctgtggcacg ggtccaggac caccaacttt
     5521 gctgggatcc tgtcccaggg tcttcggata gccccgcctg aagcgcccgt gacaggctac
     5581 atgtttggta aagggatcta tttcgctgac atggtctcca agagtgccaa ctactgccat
     5641 acgtctcagg gagacccaat aggcttaatc ctgttgggag aagttgccct tggaaacatg
     5701 tatgaactga agcacgcttc acatatcagc aagttaccca agggcaagca cagtgtcaaa
     5761 ggtttgggca aaactacccc tgatccttca gctaacatta gtctggatgg tgtagacgtt
     5821 cctcttggga ccgggatttc atctggtgtg aatgacacct ctctactata taacgagtac
     5881 attgtctatg atattgctca ggtaaatctg aagtatctgc tgaaactgaa attcaatttt
     5941 aagacctccc tgtggtaact cgacggtacc gcgggcccgg gatccaccgg atctagataa
     6001 ctgatcataa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
     6061 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
     6121 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
     6181 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
     6241 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
     6301 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
     6361 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
     6421 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
     6481 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagcccaa gcttaccatg
     6541 accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc
     6601 accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga tccggaccgc
     6661 cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc
     6721 ggcaaggtgt gggtcgcgga cgacggcgcc gcggtggcgg tctggaccac gccggagagc
     6781 gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt gagcggttcc
     6841 cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc caaggagccc
     6901 gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg tctgggcagc
     6961 gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag
     7021 acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt caccgccgac
     7081 gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc
     7141 ccgccccacg acccgcagcg cccgaccgaa aggagcgcac gaccccatgc atcggcgtct
     7201 cgatcgagat atcagtggtc caggctctag ttttgactca acaatatcac cagctgaagc
     7261 ctatagagta cgagccatag ataaaataaa agattttatt tagtctccag aaaaaggggg
     7321 gaatgaaaga ccccacctgt aggtttggca agctactagc ttaagtaacg ccattttgca
     7381 aggcatggaa aaatacataa ctgagaatag agaagttcag atcaaggtca ggaacagatg
     7441 gaacagggtc gatcgaccct agagaaccat cagatgtttc cagggtgccc caaggacctg
     7501 aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt ctgttcgcgc
     7561 gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcgggg cgccagtcct
     7621 ccgattgact gagtcgcccg ggtacccgtg tatccaataa accctcttgc agttgcatcc
     7681 gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact acccgtcagc
     7741 gggggtcttt catttggggg ctcgtccggg atcgggagac ccctgcccag ggaccaccga
     7801 cccaccaccg ggaggtaagc tggctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     7861 tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     7921 caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     7981 tcacgtagcg atagcggagt gtagatccgg ctgtggaatg tgtgtcagtt agggtgtgga
     8041 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8101 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     8161 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     8221 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     8281 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     8341 ttttgcaaaa agcttactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     8401 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgct
     8461 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     8521 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     8581 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     8641 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     8701 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     8761 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     8821 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     8881 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     8941 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     9001 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     9061 ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg aagccagtta
     9121 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     9181 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     9241 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     9301 tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta
     9361 aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg
     9421 aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg
     9481 tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc
     9541 gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg
     9601 agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg
     9661 aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctgcag
     9721 gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat
     9781 caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc
     9841 cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc
     9901 ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa
     9961 ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaacac
    10021 gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt
    10081 cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc
    10141 gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa
    10201 caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca
    10261 tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat
    10321 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
    10381 aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat aaaaataggc
    10441 gtatcacgag gccctttcgt cttcaagaat tagcttggcc attgcatacg ttgtatccat
    10501 atcataatat gtacatttat attggctcat gtccaacatt accgccatgt tgacattgat
    10561 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
    10621 agt
//
