LOCUS       sequence-428769-       14937 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Escherichia coli–Aspergillus spp. shuttle cosmid-based library 
            system.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14937)
  AUTHORS   Kadooka C, Oka T
  TITLE     Construction of a Cosmid-Based Ultraefficient Genomic Library System
            for Filamentous Fungi of the Genus Aspergillus.
  JOURNAL   J Fungi (Basel). 2024 Feb 29;10(3):188. doi: 10.3390/jof10030188.
  PUBMED    38535197
REFERENCE   2  (bases 1 to 14937)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Fungi 
            (Basel)."; date: "2024-02-29"; volume: "10(3):188. doi"; pages: " 
            10.3390/jof10030188"
COMMENT     Sequence Label: pTOCK1
FEATURES             Location/Qualifiers
     source          1..14937
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      950..959
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     1801..1819
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     1846..1866
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        1848..1866
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(1870..1889)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1871..1889)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4878..4898)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4883..5212
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5063..5198
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      5214..10462
                     /label=AMA1
                     /note="replication origin from Aspergillus nidulans 
                     (Aleksenko and Clutterbuck, 1996)"
     CDS             10814..11608
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10868..10887)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11478..11497
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     intron          12028..12093
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             12223..12243
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    12668..12802
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12705..12724)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12759..12778
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(13016..13033)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13187..13775)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13267..13286)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13946..14737,14738..14806))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14569..14588
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14807..14911)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tatggatctc agaacaatat accagaaaat gcgaaggtaa gtgcttctat attgatcctt
       61 agtgctttca aactgtgatg tagaagttgc tcggtagctg attaaatatt ctagacccaa
      121 gccgctgctg gaattgacat tattatggcc gatagggttg ggcttattgc tatgtccctg
      181 aaaggatatc aaaagcaggc aaaaagccag gcataatccc cgcgtggacg gtaccctaag
      241 gataggccct aatcttatct acatgtgact gcatcgatgt gtttggtcaa aatgaggcat
      301 gtggctcacc ccacaggcgg agaaacgtgt ggctagtgca tgacagtccc ctccatagat
      361 tcaatttaat ttttcgcggc aattgtcgtg cagtttgtat ctacatttca ttccatatat
      421 caagagttag tagttggaca tcctgattat tttgtctaat tactgaaaac tcgaagtact
      481 aacctactaa taagccagtt tcaaccacta agtgctcatt tatacaatat ttgcagaacc
      541 ccgcgctacc cctccatcgc caacatgtct tccaagtcgc aattgaccta cagcgcacgc
      601 gctagcaagc accccaatgc gctcgtaaag aagctcttcg aggttgccga ggccaagaaa
      661 accaatgtca ccgtttccgc cgacgtgaca accaccaaag agctgctgga tttggctgac
      721 cgtatgcgca ccggggatgc cacttacatg tgatctagta atggttaatg gtggattata
      781 taacaggact cggtccgtac attgccgtga tcaaaactca catcgatatc ctctccgatt
      841 tcagcgaaga aaccatcacc ggtctgaagg cccttgcaga gaagcacaat ttcctcatct
      901 tcgaagatcg caagttcatc gatatcggaa acacagtcca aaagcagtac catggcggca
      961 ctctgcgtat ctctgagtgg gcccacatca tcaactgcag tattctgccc ggtgagggta
     1021 tcgtcgaggc tctggcccag actgcttcgg ccgaggactt cccctacggc tccgagaggg
     1081 gccttttgat ccttgcggag atgacctcca agggatcttt ggctaccggt caatatacta
     1141 cttcttctgt tgactatgct cggaagtata agaagtttgt gatgggattc gtctcgacac
     1201 gtcaccttgg cgaggttcag tctgaagtta gctcgccttc ggaggaggaa gattttgtcg
     1261 tcttcacgac aggtgtcaac ctctcctcga agggtgacaa gctgggacag cagtaccaaa
     1321 ctcctgagtc ggctgttgga cgcggtgccg actttattat tgctggccgt ggaatttatg
     1381 ctgctcctga tcccgtggag gcggcgaagc agtaccagaa ggagggatgg gatgcatacc
     1441 tgaagcgtgt tggtgcgcaa taagtagtgg tggatacgta ctccttttat ggcagtatgt
     1501 cgcaagtatg atgcgattta taaattcagc actcgaaatg actactacta tgtgtctacg
     1561 acagataccc tctccgtacg aataagacac ctgcctcgat atatggacaa attcaaaatc
     1621 agggtcaagg gtcatgtttc aaagtcacaa caatctccaa catagacgag aatttgtacc
     1681 ggagtgtctg aaggtgcagc tggagattgg tctattttct tagagtgggg tatcactaat
     1741 gtacagtcgg tcactatcgt acactaagaa accattatta tcatgacatt aacctataaa
     1801 aataggcgta tcacgaggcc ctttcgtctt caagaattcg cggccgcaat taaccctcac
     1861 taaagggatc cctatagtga gtcgtattat gcggccgcga attctcatgt ttgaccgctt
     1921 atcatcgata agctctgctt tttgttgact tccattgttc attccacgga caaaaacaga
     1981 gaaaggaaac gacagaggcc aaaaagctcg ctttcagcac ctgtcgtttc ctttcttttc
     2041 agagggtatt ttaaataaaa acattaagtt atgacgaaga agaacggaaa cgccttaaac
     2101 cggaaaattt tcataaatag cgaaaacccg cgaggtcgcc gccccgtaac aaggcggatc
     2161 gccggaaagg acccgcaaat gataataatt atcaattgca tactatcgac ggcactgctg
     2221 ccagataaca ccaccgggga aacattccat catgatggcc gtgcggacat aggaagccag
     2281 ttcatccatc gctttcttgt ctgctgccat ttgctttgtg acatccagcg ccgcacattc
     2341 agcagcgttt ttcagcgcgt tttcgatcaa cgtttcaatg ttggtatcaa caccaggttt
     2401 aactttgaac ttatcggcac tgacggttac cttgttctgc gctggctcat cacgctggat
     2461 accaaggctg atgttgtaga tattggtcac cggctgaggt gtttcgattg ccgctgcgtg
     2521 gatagcacca tttgcgatag cggcgtcctt gatgaatgac actccattgc gaataagttc
     2581 gaaggagacg gtgtcacgaa tgcgctggtc cagctcgtcg attgcctttt gtgcagcaga
     2641 ggtatcaatc tcaacgccaa gcgtcatcga agcgcaatat tgctgctcac caaaacgcgt
     2701 attgaccagg tgttcaacgg caaatttctg cccttctgat gtcagaaagg taaagtgatt
     2761 ttctttctgg tattcagttg ctgtgtgtct ggtttcagca aaaccaagct cgcgcaattc
     2821 ggctgtgcca gatttagaag gcagatcacc agacagcaac gcgccacgga aaaacagcgc
     2881 atacagaaca tccgtcgccg cgccggacaa cgtgataatt ttatgaccca tgatttattt
     2941 ccttttagac gtgagcctgt cgcacagcaa agccgccgaa agttcctcga agctagcttc
     3001 agacgtgcac gtgtacgtct gctttttgtt gacttccatt gttcattcca cggacaaaaa
     3061 cagagaaagg aaacgacaga ggccaaaaag ctcgctttca gcacctgtcg tttcctttct
     3121 tttcagaggg tattttaaat aaaaacatta agttatgacg aagaagaacg gaaacgcctt
     3181 aaaccggaaa attttcataa atagcgaaaa cccgcgaggt cgccgccccg taacaaggcg
     3241 gatcgccgga aaggacccgc aaatgataat aattatcaat tgcatactat cgacggcact
     3301 gctgccagat aacaccaccg gggaaacatt ccatcatgat ggccgtgcgg acataggaag
     3361 ccagttcatc catcgctttc ttgtctgctg ccatttgctt tgtgacatcc agcgccgcac
     3421 attcagcagc gtttttcagc gcgttttcga tcaacgtttc aatgttggta tcaacaccag
     3481 gtttaacttt gaacttatcg gcactgacgg ttaccttgtt ctgcgctggc tcatcacgct
     3541 ggataccaag gctgatgttg tagatattgg tcaccggctg aggtgtttcg attgccgctg
     3601 cgtggatagc accatttgcg atagcggcgt ccttgatgaa tgacactcca ttgcgaataa
     3661 gttcgaagga gacggtgtca cgaatgcgct ggtccagctc gtcgattgcc ttttgtgcag
     3721 cagaggtatc aatctcaacg ccaagcgtca tcgaagcgca atattgctgc tcaccaaaac
     3781 gcgtattgac caggtgttca acggcaaatt tctgcccttc tgatgtcaga aaggtaaagt
     3841 gattttcttt ctggtattca gttgctgtgt gtctggtttc agcaaaacca agctcgcgca
     3901 attcggctgt gccagattta gaaggcagat caccagacag caacgcgcca cggaaaaaca
     3961 gcgcatacag aacatccgtc gccgcgccgg acaacgtgat aattttatga cccatgattt
     4021 atttcctttt agacgtgagc ctgtcgcaca gcaaagccgc cgaaagttcc tcgaccgatg
     4081 cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc
     4141 gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc
     4201 tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt
     4261 gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa
     4321 cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc
     4381 ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc
     4441 ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat
     4501 cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat cattggaccg
     4561 ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt
     4621 gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg
     4681 gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa
     4741 ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca
     4801 tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt
     4861 cctgcagatc cggctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4921 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4981 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     5041 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     5101 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     5161 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttat
     5221 tttttgtata ctgttttgtg atagcacgaa gtttttccac ggtatcttgt taaaaatata
     5281 tatttgtggc gggcttacct acatcaaatt aataagagac taattataaa ctaaacacac
     5341 aagcaagcta ctttagggta aaagtttata aatgcttttg acgtataaac gttgcttgta
     5401 tttattatta caattaaagg tggatagaaa acctagagac tagttagaaa ctaatctcag
     5461 gtttgcgtta aactaaatca gagcccgaga ggttaacaga acctagaagg ggactagata
     5521 tccgggtagg gaaacaaaaa aaaaaaacaa gacagccaca tattagggag actagttaga
     5581 agctagttcc aggactagga aaataaaaga caatgatacc acagtctagt tgacaactag
     5641 atagattcta gattgaggcc aaagtctctg agatccaggt tagttgcaac taatactagt
     5701 tagtatctag tctcctataa ctctgaagct agaataactt actactatta tcctcaccac
     5761 tgttcagctg cgcaaacgga gtgattgcaa ggtgttcaga gactagttat tgactagtca
     5821 gtgactagca ataactaaca aggtattaac ctaccatgtc tgccatcacc ctgcacttcc
     5881 tcgggctcag cagccttttc ctcctcattt tcatgctcat tttccttgtt taagactgtg
     5941 actagtcaaa gactagtcca gaaccacaaa ggagaaatgt cttaccactt tcttcattgc
     6001 ttgtctcttt tgcattatcc atgtctgcaa ctagttagag tctagttagt gactagtccg
     6061 acgaggactt gcttgtctcc ggattgttgg aggaactctc cagggcctca agatccacaa
     6121 cagagccttc tagaagactg gtcaataact agttggtctt tgtctgagtc tgacttacga
     6181 ggttgcatac tcgctccctt tgcctcgtca atcgatgaga aaaagcgcca aaactcgcaa
     6241 tatggctttg aaccacacgg tgctgagact agttagaatc tagtcccaaa ctagcttgga
     6301 tagcttacct ttgccctttg cgttgcgaca ggtcttgcag ggtatggttc ctttctcacc
     6361 agctgattta gctgccttgc taccctcacg gcggatctgc ataaagagtg gctagaggtt
     6421 ataaattagc actgatccta ggtacggggc tgaatgtaac ttgcctttcc tttctcatcg
     6481 cgcggcaaga caggcttgct caaattccta ccagtcacag gggtatgcac ggcgtacgga
     6541 ccacttgaac tagtcacaga ttagttagca actagtctgc attgaatggc tgtacttacg
     6601 ggccctcgcc attgtcctga tcatttccag cttcaccctc gttgctgcaa agtagttagt
     6661 gactagtcaa ggactagttg aaatgggaga agaaactcac gaattctcga cacccttagt
     6721 attgtggtcc ttggacttgg tgctgctata tattagctaa tacactagtt agactcacag
     6781 aaacttacgc agctcgcttg cgcttcttgg taggagtcgg ggttgggaga acagtgcctt
     6841 caaacaagcc ttcataccat gctacttgac tagtcaggga ctagtcacca agtaatctag
     6901 ataggacttg cctttggcct ccatcagttc cttcatagtg ggaggtccat tgtgcaatgt
     6961 aaactccatg ccgtgggagt tcttgtcctt caagtgcttg accaatatgt ttctgttggc
     7021 agagggaacc tgtcaactag ttaataacta gtcagaaact agtatagcag tagactcact
     7081 gtacgcttga ggcatccctt cactcggcag tagacttcat atggatggat atcaggcacg
     7141 ccattgtcgt cctgtggact agtcagtaac taggcttaaa gctagtcggg tcggcttact
     7201 atcttgaaat ccggcagcgt aagctccccg tccttaactg cctcgagata gtgacagtac
     7261 tctggggact ttcggagatc gttatcgcga atgctcggca tactaatcgt tgactagtct
     7321 tggactagtc ccgagcaaaa aggattggag gaggaggagg aaggtgagag tgagacaaag
     7381 agcgaaataa gagcttcaaa ggctatctct aagcagtatg aaggttaagt atctagttct
     7441 tgactagatt taaaagagat ttcgactagt tatgtacctg gagtttggat ataggaatgt
     7501 gttgtggtaa cgaaatgtaa gggggaggaa agaaaaagtc ggtcaagagg taactctaag
     7561 tcggccattc ctttttggga ggcgctaacc ataaacggca tggtcgactt agagttagct
     7621 cagggaattt agggagttat ctgcgaccac cgaggaacgg cggaatgcca aagaatcccg
     7681 atggagctct agctggcggt tgacaacccc accttttggc gtttctgcgg cgttgcaggc
     7741 gggactggat acttcgtaga accagaaagg caaggcagaa cgcgctcagc aagagtgttg
     7801 gaagtgatag catgatgtgc cttgttaact aggtcaaaat ctgcagtatg cttgatgtta
     7861 tccaaagtgt gagagaggaa ggtccaaaca tacacgattg ggagagggcc taggtataag
     7921 agtttttgag tagaacgcat gtgagcccag ccatctcgag gagattaaac acgggccggc
     7981 atttgatggc tatgttagta ccccaatgga aagcctgaga gtccagtggt cgcagataac
     8041 tccctaaatt ccctgagcta actctaagtc gaccatgccg tttatggtta gcgcctccca
     8101 aaaaggaatg gccgacttag agttacctct tgaccgactt tttctttcct cccccttaca
     8161 tttcgttacc acaacacatt cctatatcca aactccaggt acataactag tcgaaatctc
     8221 ttttaaatct agtcaagaac tagatactta accttcatac tgcttagaga tagcctttga
     8281 agctcttatt tcgctctttg tctcactctc accttcctcc tcctcctcca atcctttttg
     8341 ctcgggacta gtccaagact agtcaacgat tagtatgccg agcattcgcg ataacgatct
     8401 ccgaaagtcc ccagagtact gtcactatct cgaggcagtt aaggacgggg agcttacgct
     8461 gccggatttc aagatagtaa gccgacccga ctagctttaa gcctagttac tgactagtcc
     8521 acaggacgac aatggcgtgc ctgatatcca tccatatgaa gtctactgcc gagtgaaggg
     8581 atgcctcaag cgtacagtga gtctactgct atactagttt ctgactagtt attaactagt
     8641 tgacaggttc cctctgccaa cagaaacata ttggtcaagc acttgaagga caagaactcc
     8701 cacggcatgg agtttacatt gcacaatgga cctcccacta tgaaggaact gatggaggcc
     8761 aaaggcaagt cctatctaga ttacttggtg actagtccct gactagtcaa gtagcatggt
     8821 atgaaggctt gtttgaaggc actgttctcc caaccccgac tcctaccaag aagcgcaagc
     8881 gagctgcgta agtttctgtg agtctaacta gtgtattagc taatatatag cagcaccaag
     8941 tccaaggacc acaatactaa gggtgtcgag aattcgtgag tttcttctcc catttcaact
     9001 agtccttgac tagtcactaa ctactttgca gcaacgaggg tgaagctgga aatgatcagg
     9061 acaatggcga gggcccgtaa gtacagccat tcaatgcaga ctagttgcta actaatctgt
     9121 gactagttca agtggtccgt acgccgtgca tacccctgtg actggtagga atttgagcaa
     9181 gcctgtcttg ccgcgcgatg agaaaggaaa ggcaagttac attcagcccc gtacctagga
     9241 tcagtgctaa tttataacct ctagccactc tttatgcaga tccgccgtga gggtagcaag
     9301 gcagctaaat cagctggtga gaaaggaacc ataccctgca agacctgtcg caacgcaaag
     9361 ggcaaaggta agctatccaa gctagtttgg gactagattc taactagtct cagcaccgtg
     9421 tggttcaaag ccatattgcg agttttggcg ctttttctca tcgattgacg aggcaaaggg
     9481 agcgagtatg caacctcgta agtcagactc agacaaagac caactagtta ttgaccagtc
     9541 ttctagaagg ctctgttgtg gatcttgagg ccctggagag ttcctccaac aatccggaga
     9601 caagcaagtc ctcgtcggac tagtcactaa ctagactcta actagttgca gacatggata
     9661 atgcaaaaga gacaagcaat gaagaaagtg gtaagacatt tctcctttgt ggttctggac
     9721 tagtctttga ctagtcacag tcttaaacaa ggaaaatgag catgaaaatg aggaggaaaa
     9781 ggctgctgag cccgaggaag tgcagggtga tggcagacat ggtaggttaa taccttgtta
     9841 gttattgcta gtcactgact agtcaataac tagtctctga acaccttgca atcactccgt
     9901 ttgcgcagct gaacagtggt gaggataata gtagtaagtt attctagctt cagagttata
     9961 ggagactaga tactaactag tattagttgc aactaacctg gatctcagag actttggcct
    10021 caatctagaa tctatctagt tgtcaactag actgtggtat cattgtcttt tattttccta
    10081 gtcctggaac tagcttctaa ctagtctccc taatatgtgg ctgtcttgtt tttttttttt
    10141 gtttccctac ccggatatct agtccccttc taggttctgt taacctctcg ggctctgatt
    10201 tagtttaacg caaacctgag attagtttct aactagtctc taggttttct atccaccttt
    10261 aattgtaata ataaatacaa gcaacgttta tacgtcaaaa gcatttataa acttttaccc
    10321 taaagtagct tgcttgtgtg tttagtttat aattagtctc ttattaattt gatgtaggta
    10381 agcccgccac aaatatatat ttttaacaag ataccgtgga aaaacttcgt gctatcacaa
    10441 aacagtatac aaaaaataag cttcacgctg ccgcaagcac tcagggcgca agggctgcta
    10501 aaggaagcgg aacacgtaga aagccagtcc gcagaaacgg tgctgacccc ggatgaatgt
    10561 cagctactgg gctatctgga caagggaaaa cgcaagcgca aagagaaagc aggtagcttg
    10621 cagtgggctt acatggcgat agctagactg ggcggtttta tggacagcaa gcgaaccgga
    10681 attgccagct ggggcgccct ctggtaaggt tgggaagccc tgcaaagtaa actggatggc
    10741 tttcttgccg ccaaggatct gatggcgcag gggatcaaga tctgatcaag agacaggatg
    10801 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
    10861 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
    10921 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
    10981 cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
    11041 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
    11101 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
    11161 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
    11221 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
    11281 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
    11341 gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
    11401 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
    11461 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
    11521 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
    11581 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
    11641 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
    11701 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
    11761 ctggagttct tcgcccaccc cgggctcgat cccctcgcga gttggttcag ctgctgcctg
    11821 aggctggacg acctcgcgga gttctaccgg cagtgcaaat ccgtcggcat ccaggaaacc
    11881 agcagcggct atccgcgcat ccatgccccc gaactgcagg agtggggagg cacgatggcc
    11941 gctttggtcc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
    12001 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
    12061 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
    12121 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
    12181 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
    12241 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
    12301 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
    12361 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
    12421 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
    12481 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
    12541 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
    12601 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
    12661 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
    12721 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
    12781 tgtatcttat catgtctgga tctgacgggt gcgcatgatc gtgctcctgt cgttgaggac
    12841 ccggctaggc tggcggggtt gccttactgg ttagcagaat gaatcaccga tacgcgagcg
    12901 aacgtgaagc gactgctgct gcaaaacgtc tgcgacctga gcaacaacat gaatggtctt
    12961 cggtttccgt gtttcgtaaa gtctggaaac gcggaagtca gcgctcttcc gcttcctcgc
    13021 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    13081 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    13141 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    13201 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    13261 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    13321 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    13381 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    13441 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    13501 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    13561 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    13621 ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    13681 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    13741 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    13801 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    13861 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    13921 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    13981 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    14041 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    14101 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    14161 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    14221 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
    14281 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    14341 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    14401 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    14461 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    14521 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
    14581 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    14641 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    14701 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    14761 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    14821 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    14881 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctg
//
