LOCUS       sequence-428795-       11368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses SM(FLAG)-nonoptimal Firefly luciferase-optimal GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Barrington CL, Galindo G, Koch AL, Horton ER, Morrison EJ, Tisa S, 
            Stasevich TJ, Rissland OS
  TITLE     Synonymous codon usage regulates translation initiation.
  JOURNAL   Cell Rep. 2023 Dec 26;42(12):113413. doi: 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12.
  PUBMED    38096059
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2023-12-26"; volume: "42(12):113413. doi"; pages: " 
            10.1016/j.celrep.2023.113413. Epub 2023 Dec 12"
COMMENT     Sequence Label: pUB_SM-FLuc0-GFPOPT
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(113..132)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      926..1514
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1415..1434
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1668..1685
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1925..1946
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1961..1966,1967..1984,1985..1991)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1999..2015
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2004..2026
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2023..2039
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2023..2039
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2089..2108
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             2300..2933
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    2980..3105
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3602..3835
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4020..4064
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    4357..4474
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4517..4915
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     regulatory      4921..4930
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4930..4953
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4957..4980
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             4984..5007
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5512..5535
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5542..5565
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5578..5601
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5608..5631
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5824..5847
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5851..5874
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             5878..5901
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    10057..10645
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10110..10130)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10528..10539)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             10720..10953
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(11072..11090)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        11158..11262
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11263..11331,11332..11368,1..755)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
       61 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      121 gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc atacactatt
      181 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      241 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
      301 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
      361 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
      421 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
      481 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
      541 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
      601 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
      661 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
      721 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
      781 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
      841 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
      901 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
      961 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1021 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1081 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1141 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1201 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1261 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1321 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1381 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1441 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1501 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1561 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1621 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     1681 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     1741 attaatgcag caagctcatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     1801 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg
     1861 caaaaagctc cccgtggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     1921 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     1981 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca
     2041 tgattacgaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     2101 aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc
     2161 atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac
     2221 tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat
     2281 gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt
     2341 gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg
     2401 ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca
     2461 gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg
     2521 catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca
     2581 tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct
     2641 tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg
     2701 gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct
     2761 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     2821 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     2881 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     2941 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     3001 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     3061 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     3121 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     3181 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     3241 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     3301 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     3361 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     3421 agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata
     3481 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     3541 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     3601 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     3661 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     3721 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     3781 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     3841 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     3901 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     3961 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     4021 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     4081 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     4141 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     4201 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     4261 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     4321 atcgccgaat tcacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     4381 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     4441 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     4501 tccagtttgg actagtggcc tccgcgccgg gttttggcgc ctcccgcggg cgcccccctc
     4561 ctcacggcga gcgctgccac gtcagacgaa gggcgcagcg agcgtcctga tccttccgcc
     4621 cggacgctca ggacagcggc ccgctgctca taagactcgg ccttagaacc ccagtatcag
     4681 cagaaggaca ttttaggacg ggacttgggt gactctaggg cactggtttt ctttccagag
     4741 agcggaacag gcgaggaaaa gtagtccctt ctcggcgatt ctgcggaggg atctccgtgg
     4801 ggcggtgaac gccgatgatt atataaggac gcgccgggtg tggcacagct agttccgtcg
     4861 cagccgggat ttgggtcgcg gttcttgttt gtggatcgct gtgatcgtca cttggcggcc
     4921 gccaccatgg actacaagga cgacgacgac aaaggtgact acaaagatga tgacgataaa
     4981 ggcgactata aggacgatga cgacaagggc ggaaactcac tgatcaagga aaacatgcgg
     5041 atgaaggtgg tgatggaggg ctccgtgaat ggtcaccagt tcaagtgcac cggagaggga
     5101 gagggaaacc cgtacatggg aactcagacc atgcgcatta aggtcatcga aggaggtccg
     5161 ctgccgttcg ctttcgatat cctggccact tcgttcggag gagggtcgcg cacgttcatc
     5221 aagtacccga agggaatccc ggacttcttt aagcagtcat tcccggaagg attcacttgg
     5281 gaacgggtga cccggtatga agatggaggt gtggtgactg tcatgcaaga tacttcgctg
     5341 gaggatgggt gcctcgtgta ccacgtccaa gtccgcggag tgaatttccc gtccaacgga
     5401 ccagtgatgc agaaaaagac gaagggttgg gaacctaata ctgaaatgat gtaccccgca
     5461 gacggagggc tgaggggcta cacccacatg gcgctgaagg tcgacggagg agattacaag
     5521 gatgacgacg ataagcaaca agattacaaa gacgatgatg acaagggcca gcagggcgac
     5581 tacaaggacg acgacgacaa gcagcaggac tacaaagatg acgatgataa aggaggagga
     5641 catctgtcct gttcgttcgt gaccacctac agatcaaaga aaaccgtggg aaacatcaag
     5701 atgccgggca ttcatgccgt cgaccaccgc ctggagcggc tcgaagaatc agacaatgag
     5761 atgttcgtcg tgcaaagaga acatgccgtg gccaagttcg cgggactggg aggcggtgga
     5821 ggcgattaca aagacgatga tgacaagggt gactataaag acgacgatga caaaggggat
     5881 tacaaggatg atgatgataa gggaggcggt ggatcaggtg gaggaggttc actgcaggaa
     5941 gatgcgaaga atataaagaa gggacccgcg cctttttatc ccctagaaga tggaaccgcg
     6001 ggagaacaac tacataaggc aatgaagaga tatgcactag tacctggaac aatagcattt
     6061 acagatgcac atatagaagt cgatataacg tatgcggaat attttgaaat gtccgtccgt
     6121 ctcgcagaag cgatgaagag atatgggcta aatacaaatc atagaatagt cgtatgtagt
     6181 gaaaattctc ttcaattttt tatgcctgtc ctcggggcgc tatttatagg agtcgcagtc
     6241 gcgcccgcga atgatatata taatgaacgt gaactcctca atagtatggg aatatcgcaa
     6301 cctaccgtag tatttgtatc caagaagggg ctccaaaaga tactcaatgt ccaaaagaag
     6361 ctccccataa tccaaaagat cataataatg gattctaaga cggattatca aggatttcaa
     6421 tcgatgtata cgtttgtcac atctcatcta cctcccggat ttaatgaata tgattttgtc
     6481 cctgaatcct ttgataggga taagacaata gcactaataa tgaattcctc tggatctacc
     6541 ggacttccta agggggtcgc acttcctcat agaaccgcat gtgtcagatt ttcgcatgcg
     6601 agagatccta tatttggcaa tcaaataata cctgatacag cgatacttag tgtcgtccct
     6661 tttcatcatg ggtttggaat gtttactaca ctcggatatt tcatatgtgg atttcgcgtc
     6721 gtcctcatgt atagatttga agaagaactc tttctaaggt cccttcaaga ttataagata
     6781 caaagtgcgc tcctcgtccc taccctattc tccttttttg caaagagtac ccttatagat
     6841 aagtatgatc tttctaatct acatgaaata gcatctgggg gagcgcccct ctctaaggaa
     6901 gtcggggaag cggtagcaaa gaggtttcat ctacctggga taaggcaagg atatgggctc
     6961 acagaaacca caagtgcgat actaataaca cccgaagggg atgataagcc tggagcggtc
     7021 gggaaggtag tacccttttt tgaagcgaag gtcgtagatc ttgataccgg gaagacgctt
     7081 ggagtcaatc aaagagggga actctgtgtc agaggaccta tgataatgtc cggatatgta
     7141 aataatcccg aagcgaccaa tgcgctaata gataaggatg gatggctaca ttctggagat
     7201 atagcgtatt gggatgaaga tgaacatttt tttatagttg accgcctaaa gtctctcata
     7261 aagtataagg ggtatcaagt agcacccgca gaacttgaat ccatactact ccaacatccc
     7321 aatatatttg atgcaggggt cgcaggactt cccgatgatg atgcggggga acttcccgca
     7381 gcggtcgtcg tcctcgaaca tggaaagacg atgacggaaa aggaaatagt agattatgtc
     7441 gcaagtcaag taacaaccgc gaagaagctt cgcggaggag tcgtatttgt cgacgaagta
     7501 cccaaggggc ttaccggaaa gctcgatgca agaaagataa gagaaatact cataaaggca
     7561 aagaaggggg gaaagatagc agtaggtacc tatacgcgtg tgagtaaagg cgaggagctg
     7621 ttcactggcg tggttccaat tctggttgag ctggacggtg acgtgaacgg tcacaaattc
     7681 tcagttagcg gcgagggtga gggcgacgct acctacggta aactgacctt gaaattcatt
     7741 tgcactactg gtaaattacc agttccatgg ccaactttag ttactacttt gacctacggt
     7801 gttcagtgct tcagtcggta cccggaccac atgaaacagc acgacttctt caaatcagct
     7861 atgccagagg gctacgtgca ggagcgtacc attttcttca aagacgacgg taactacaaa
     7921 acccgagctg aggttaaatt cgagggcgac accttggtta accgtattga gttgaaaggt
     7981 attgacttca aagaggacgg caacattttg ggccacaaat tagagtacaa ctacaacagc
     8041 cacaacgttt acattatggc tgacaaacag aaaaacggca ttaaagtgaa cttcaaaatt
     8101 cggcacaaca ttgaggacgg cagcgttcag ctggccgacc actaccagca gaacacccca
     8161 attggtgacg gtccagtgct gttgccggac aaccactacc tgagcactca gagcgcctta
     8221 agtaaagacc caaacgagaa acgtgaccac atggtgttat tggagttcgt gactgctgct
     8281 ggcattaccc tgggcatgga cgagtaaatg catcagccgc tagctaggcg gactgttact
     8341 gagctgcgtt ttacaccctt tctttgacaa aacctaactt gcgcagaaaa aaaaaaaata
     8401 agagacaaca ttggcatggc tttgtttttt taaatttttt ttaaagtttt tttttttttt
     8461 tttttttttt aagttttttt gttttgtttt ggcgcttttg actcaggatt taaaaactgg
     8521 aacggtgaag gcgacagcag ttggttggag caaacatccc ccaaagttct acaaatgtgg
     8581 ctgaggactt tgtacattgt tttgtttttt ttttttttgg ttttgtcttt ttttaatagt
     8641 cattccaagt atccatgaaa taagtggtta caggaagtcc ctcaccctcc caaaagccac
     8701 ccccactcct aagaggagga tggtcgcgtc catgccctga gtccaccccg gggaaggtga
     8761 caaccggtaa cctacaaacg ggtggaggat caccccaccc gacacttcac aatcaagggg
     8821 tacaatacac aagggtggag gaacacccca ccctccagac acattacaca gaaatccaat
     8881 caaacagaag caccatcagg gcttctgcta ccaaatttat ctcaaaaaac tacaacaagg
     8941 aatcaccatc agggattccc tgtgcaatat acgtcaaacg agggccacga cgggaggacg
     9001 atcacgcctc ccgaatatcg gcatgtctgg ctttcgaatt cagtgcgtgg agcatcagcc
     9061 cacgcagcca atcagagtcg aatacaagtc gactttcgcg aagagcatca gccttcgcgc
     9121 cattcttaca caaaccacac tctcccctac aggaacagca tcagcgttcc tgcccagtac
     9181 ccaactcaag aaaatttatg tccccatgca gcatcagcgc atgggcccca agaatacatc
     9241 cccaacaaaa tcacatccga gcaccaacag ggctcggagt gttgtttctt gtccaactgg
     9301 acaaaccctc catggaccat caggccatgg actctcacca acaagacaaa aactactctt
     9361 ctcgaagcag catcagcgct tcgaaacact cgagcataca ttgtgcctat ttcttgggtg
     9421 gacgatcacg ccacccatgc tctcacgaat ttcaaaacac ggacaaggac gagcaccacc
     9481 agggctcgtc gttccacgtc caatacgatt acttaccttt cgggatcacg atcacggatc
     9541 ccgcagctac atcacttcca ctcaggacat tcaagcatgc acgatcacgg catgctccac
     9601 aagtctcaac cacagaaact accaaatggg ttcagcacca gcgaacccac tcctacctca
     9661 aacctcttcc cacaaaactg gcaagcagga tcaccgcttg cccattccaa cataccaaat
     9721 caaaaacaat tactggtaca gcatcagcgt accagcccac atctctcact actatcaaaa
     9781 accaaaccgt tcagcaacag cgaacggtac acacggaaaa atcaactggt ttacaaatac
     9841 gaaagacgat cacgctttcg tccagcgcaa actattacga aaaacatccg acgggaagag
     9901 caacagcctt cccgcggcgg aaaacctcac aaaaacacga caaacggatg cacgaacacg
     9961 gcatccgccg acaacccaca aacttacaac caggcaaacg gtgcaggatc accgcaccgt
    10021 acatcaaaca cctcagatct catatcgata gatcctaatc aacctctgga ttacaaaatt
    10081 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
    10141 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
    10201 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
    10261 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
    10321 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
    10381 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
    10441 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
    10501 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
    10561 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
    10621 atctcccttt gggccgcctc cccgcctgag atcctttaag accaatgact tacaaggcag
    10681 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
    10741 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
    10801 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
    10861 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
    10921 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
    10981 agtatttata acttgcaaag aaatgaatat cagagagtga gaggcccggg ttaattaagg
    11041 aaagggctag atcattcttg aagacgaaag ggcctcgtga tacgcctatt tttataggtt
    11101 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
    11161 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
    11221 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
    11281 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
    11341 acgctggtga aagtaaaaga tgctgaag
//
