LOCUS       sequence-428824-        8705 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FRET-based reporter for Ras activity, mitochondria localized.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8705)
  AUTHORS   Zhang JZ, Nguyen WH, Greenwood N, Rose JC, Ong SE, Maly DJ, Baker D
  TITLE     Computationally designed sensors detect endogenous Ras activity and 
            signaling effectors at subcellular resolution.
  JOURNAL   Nat Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w.
  PUBMED    38273065
REFERENCE   2  (bases 1 to 8705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Biotechnol. 2024 Jan 25. doi: 10.1038/s41587-023-02107-w."
COMMENT     Sequence Label: pcDNA3 Mito-Ras-LOCKR-S
FEATURES             Location/Qualifiers
     source          1..8705
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      897..906
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1002..1019
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             1026..1055
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1065..1094
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             1104..1133
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2040..2096
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             2097..2120
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2130..2153
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             2163..2186
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4296..4313)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4302..4526
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4572..5000
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4659..4678)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4869..4890
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5009..5029)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5014..5343
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5194..5329
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5256..5275
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5410..6204
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5464..5483)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6074..6093
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6378..6499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6415..6434)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6469..6488
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6548..6564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6548..6564)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6561..6583)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6572..6588
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6596..6602,6603..6620,6621..6626))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6641..6662
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6779..6796)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6950..7538)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7030..7049)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7709..8500,8501..8569))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8332..8351
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8570..8674)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttgcgcca
      901 ccatggcaat ccaattgcgt tcgctcttcc ccttggcgtt gcccggaatg ctggccctcc
      961 ttggctggtg gtggtttttc tctcgtaaaa aagggtctag ccatcaccat catcaccatt
     1021 ctagcgagca gaaactcatc tctgaagagg atctcggtgg tagtgaacaa aagttgatat
     1081 ccgaggagga tttgggtggg agcgagcaga aacttatcag tgaagaagat ttggggggct
     1141 ccatggatcc tgacgaggca aggaaagcca tagcgagggt taaaagggaa agcaaacgaa
     1201 tagtagaaga cgcggaaagg cttagtcgag aagcagcggc ggcaagtgag aaatcctccc
     1261 gagaggcgga gaggctctcc cgggaggccg ctgctgcgtc cgagaaaatt tcccgggagg
     1321 gaggaacagg ggggtcagga gggactggag gctcaatggt atctaaagga gaagagctgt
     1381 ttactggggt cgtgcctatc ctggtagagc tggacggtga cgttaatgga cataagttta
     1441 gcgtaagtgg ggaaggcgaa ggcgatgcta cttacgggaa gttgactttg aaatttatct
     1501 gtaccactgg aaagctgccg gtaccctggc ctacgttggt taccacgttg acctggggtg
     1561 tccaatgctt ctcaagatac ccagatcaca tgaagcaaca cgactttttc aaatctgcga
     1621 tgcccgaagg atatgttcaa gagagaacga tattttttaa agatgatggt aactataaaa
     1681 ctagagccga ggtcaagttc gagggggaca ctctggttaa ccggatcgaa cttaaaggca
     1741 ttgactttaa ggaggatggg aacatcctgg gccataaatt ggagtacaac tatatatcac
     1801 ataacgtcta tatcacggcg gacaagcaaa agaatgggat aaaggctaac tttaaaatac
     1861 ggcataacat tgaggatgga agcgtgcagc ttgcggacca ttatcaacaa aatacgccta
     1921 taggagacgg acccgtgctg ctgcctgata atcactatct ttccactcaa agtgcgctct
     1981 ctaaagaccc gaatgagaaa cgagaccata tggttctgtt ggagttcgta acggcagcgg
     2041 caaccaattt cagtctcctg aagcaggcag gtgatgtaga agagaatccc ggaccggatt
     2101 ataaagacga tgatgacaag ggaggtagtg attacaagga tgatgacgat aaaggaggtt
     2161 ccgattacaa agatgatgac gacaagggcg ggtctatgaa gggcgaagaa cttttcacag
     2221 gcgtggtgcc gatattggtg gaactcgatg gagacgtaaa tgggcataaa ttctcagttt
     2281 ccggggaagg ggaaggtgac gccacttatg ggaagcttac tctcaaattg ctgtgcacta
     2341 cggggaagct tccagtgcct tggcctacac ttgttaccac actggggtac ggagtgcagt
     2401 gtttcgcccg ctaccccgac cacatgaaac agcatgattt ctttaaatct gcgatgccag
     2461 aaggttatgt gcaagagaga actatttttt tcaaggatga cggcaattac aaaactaggg
     2521 cggaggttaa gttcgagggt gacacactcg tgaataggat tgagttgaag ggcatagact
     2581 ttaaagaaga tgggaatatc ttgggtcaca agcttgagta taattataac tcccataatg
     2641 tctacatcac agcagataaa cagaaaaatg gaattaaagc aaacttcaag atacgacaca
     2701 acatcgagga cggtggagtt cagttggcgg accactacca acaaaataca cctatcggtg
     2761 acgggccagt ccttctgccc gacaaccact acctctccta tcaaagcgcg cttttcaagg
     2821 accctaatga gaagagagac catatggttc ttcttgaatt tctgacagca gcgggaggta
     2881 ccggcggttc cgggggtacg ggaggcagtt caaaggaagc agcgaagaaa ctccaggacc
     2941 tgaatataga gcttgcgcgg aaactcctcg aagcgagcac gaagcttcaa agacttaaca
     3001 taaggctggc agaagctctc ttggaagcca tcgctagatt gcaggagctg aatctggagc
     3061 tcgtatacct ggctgttgag ctgacagacc ctaaacggat acgggatgag atcaaggaag
     3121 tcaaagataa gagcaaggaa attattcgcc gcgctgaaaa agaaatagat gatgctgcca
     3181 aggagtccaa aaaaattctt gaggaagcac gaaaagctat tcgagacgcc gccgaggaga
     3241 gtaggaaaat tctcgaggaa ggtagcggga gcgggtcaga tgccctggat gagttgcaaa
     3301 agttgaatct tgaacttgcg aagcttctcc ttaaagcaat tgctgaaact caagacttga
     3361 atcttagagc tgcaaaggcc tttctcgaag cggccgcaaa attgcaggaa cttaatatta
     3421 gggctgtgga acttctcgta aagctcaccg acccggcaac catacgccgg gctctggagc
     3481 atgctaagag acggtcaaag gagataatcg acgaagcgga aagggctatt cgggcagcta
     3541 agcgcgagag cgagcgaatt attgaagaag cgcggcgact catagagaag gccaaggagg
     3601 agagtgaaag gataattcga gaaggttccg gcagtggtga tcccgatatc aaaaaattgc
     3661 aggatctcaa cattgaactg gcaagagagc tgctccgggc tcatgcacag cttcagcgac
     3721 ttaatttgga gttgcttcgc gaactgcttc gagctttggc tcaattgcaa gagcttaacc
     3781 tcgacctctt gaggttggct tccgaattga ctgacccaga cgaggcgagg aaagcgatcg
     3841 ccgttacagg ctaccggctc tttgaagaaa tactggacag tgagcgattg agtcgggagg
     3901 ccgcagcagc atctgaaaag agcagccggg aggctgaacg ccttatacga gaagctgcag
     3961 cggcttctag caatactata agggtttttc tccccaacaa gcaacgcacc gtggtcaacg
     4021 tgaggaacgg gatgtcactg cacgattgcc tgatgaaggc gctgaaggtt cgaggtttgc
     4081 aacccgagtg ttgcgcggtg ttcaggcttc ttcacgagca taaggggaaa aaagcacgat
     4141 tggattggaa cactgatgca gccagtctca tcggcgagga attgcaggtg gattttttgt
     4201 gagaattctg cagatatcca tcacagaatt ctgcagatat ccagcacagt ggcggccgct
     4261 cgagtctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     4321 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     4381 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     4441 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     4501 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct ggggctctag
     4561 ggggtatccc cacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     4621 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     4681 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     4741 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     4801 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     4861 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     4921 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     4981 acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc agttagggtg tggaaagtcc
     5041 ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg
     5101 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     5161 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     5221 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     5281 tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     5341 aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac aggatgagga
     5401 tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag
     5461 aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc
     5521 cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg
     5581 aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc
     5641 gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg
     5701 ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct
     5761 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg
     5821 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat
     5881 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc
     5941 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg
     6001 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc
     6061 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct
     6121 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat
     6181 cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc gaccaagcga
     6241 cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa aggttgggct
     6301 tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat ctcatgctgg
     6361 agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     6421 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     6481 aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag agcttggcgt
     6541 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     6601 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     6661 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     6721 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     6781 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     6841 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     6901 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
     6961 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     7021 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     7081 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     7141 ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     7201 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     7261 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     7321 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     7381 acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     7441 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     7501 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     7561 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     7621 caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     7681 gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     7741 cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     7801 cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     7861 caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     7921 gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     7981 gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt
     8041 cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     8101 catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     8161 gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     8221 ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     8281 gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg gataataccg
     8341 cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     8401 tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     8461 gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     8521 atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     8581 ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     8641 gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     8701 acgtc
//
