LOCUS       sequence-428835-        8904 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses melanoma differentiation-associated protein 5 lacking its 
            2 CARD domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8904)
  TITLE     MDA5 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pET50_MDA5∆2CARD
FEATURES             Location/Qualifiers
     source          1..8904
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(21..68)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     64..82
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(163..207)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(208..225)
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             complement(2479..2502)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(2512..2529)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(2545..4029)
                     /codon_start=1
                     /gene="nusA"
                     /product="transcription elongation protein (N utilization 
                     substance protein A)"
                     /label=NusA
                     /note="highly soluble in E. coli"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEIDV
                     RVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTFDR
                     ITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEAVIL
                     REDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGEEVIE
                     IKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLWDDNPA
                     QFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGWELNVMT
                     VDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKELLEIEGLD
                     EPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAARGVCTLED
                     LAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     CDS             complement(4036..4053)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             4073..4095
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    4110..4134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(4134..4153)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4135..4153)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4241..4260)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     promoter        4466..4543
                     /gene="lacI"
                     /label=lacI promoter
     CDS             4544..5626
                     /codon_start=1
                     /gene="lacI"
                     /product="lac repressor"
                     /label=lacI
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
                     NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
                     EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
                     EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
                     MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
                     YIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
                     ALADSLMQLARQVSRLESGQ"
     primer_bind     complement(4563..4582)
                     /label=LacI-R
                     /note="LacI, reverse primer"
     CDS             6198..6389
                     /codon_start=1
                     /gene="rop"
                     /product="Rop protein, which maintains plasmids at low copy
                     number"
                     /label=rop
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
                     DELYRSCLARFGDDGENL"
     primer_bind     complement(6383..6405)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    6491..6630
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     6546..6565
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(6645..6662)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6816..7404)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6896..6915)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     complement(7515..7534)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             7526..8341
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             7526..8341
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(7599..7618)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      complement(8433..8888)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8570..8591)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8782..8801
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gatatagttc ctcctttcag caaaaaaccc ctcaagaccc gtttagaggc cccaaggggt
       61 tatgctagtt attgctcagc ggtggcagca gccaactcag cttcctttcg ggctttgttt
      121 agcagcctag gttaattaag cctcgagagc agcagaagta gagctgtcca tgtgctggcg
      181 ttcgaattta gcagcagcgg tttctttact accgcgtggc accagagcga gctctgcggc
      241 cgcaagcttg tcgacctaat cctcatcact aaataaacag cattctgaat agtcaagatt
      301 gggaaatgtg ataggtaatt ctacccactt tttgtattgt ttctttgttg aattattttt
      361 gaaaaccact acaaaattcc ttattttgag acaaggcaaa tctaagcctt tgtgcaccat
      421 cattgttccc caagcctggc cacatttgca gatgatttca ccatttattt gatagtcggc
      481 acacttcttt tgcagtgttt tgttttctct tacaatgtaa agttccttga attctggggt
      541 catattgacg tgatgcattt tctcaattac atggatatct tccccagaac aggctagcac
      601 actgcagttt ttgcaaagga aagttattag tgatgggtta ttcttgtaat gcttggcaat
      661 atttctcttg gttttcattt tcttttccat tatactttgc atctgtaatt ccaaaatctt
      721 atgagcatac tcctctggtt tcatattttg aacacaatgt atagctttat acatcatctt
      781 ctctcggaaa tcattaactg tctcacgttc gataactcct gaaccactgt gagcaaccag
      841 gacgtaggtg ctctcatcag ctctggctcg accacgggcc tggaccatgg ctatttcatt
      901 ggtgacgaga ccataacgga taacaatgtt acattcttta atatccagac cttcttctgc
      961 cactgtggta gcgataagca gatttatttt tccagtgcga aatttactaa tgacttcttt
     1021 ttgttcattc tgtgtcatgg gtttgaactc actgctgtgt ccagctccaa tcagatggtg
     1081 ggctttgact cctacttcag caaatttttc attttcagta atccactggg aaagcgcata
     1141 tgcactctgt cgtgtttttg taaagattat tcctcgtgct gattcctcag tcctagtata
     1201 ttgctccatt atggtatttc ttaatttggt cagcttttca ttttcatatt ctgggttttc
     1261 agccagcctt ttcaacattt tattgttttc aaaaaataaa gtcatgagaa atctatctgt
     1321 ttcatccagt ttcaaaggtt tctttaaatc atcctcatct tcatcaccat cacaatactc
     1381 atcatcacca ccctcatcac tatcatcttc tatgactgca aacttcttat ctttctcttc
     1441 attatagaaa gtttcaagat gagtatacgc atctatcatt cgaattgtgt cattaatttg
     1501 tagggcctca ttgtacttcc tcaaatgttc tgcacaaaca cgttctttgc gatttccttc
     1561 ttttgcagct tttttttcca tttgaatggc ccattgttca tagggttgag ttccaaaatc
     1621 tgacattgga ctcatttgac aataagtttg aatccttgtc attatttcta gaagtttctc
     1681 tttaaatgga tcttctctgg ttgcatctgc aatggcaaac ttcttgcatg gctcctgtat
     1741 ttggtttttc agttgatcaa ggttttcttt aacagtttta atagtaaatg catcaagatt
     1801 ggcacatagt tttaaaatgt gttcttcagc tttggcttgc ttcgtggccc ctccaacacc
     1861 aggtgaagct gttagtccca gtatctgagg aaggggaatc actggtttgt tttctttctt
     1921 gagtctattg tttttcaact tctgcatcaa ataatgcctc atgatgttat tatacactgc
     1981 ttctttgttg gtgtgatgac attcatcaat gataatgagg gaaaagtctg acaattgaac
     2041 accagcatct tctccatttt ccaagtttaa gagggagttt tcaaggattt gagctgtact
     2101 gataataata tcacaggact tgacaacttc tggaaatgat attttcagtt gggtatcacc
     2161 acttaatcca ataacacgat accatttctt caaaaatggt tggaactcct tgcggaagag
     2221 ctgttcaact agcagtacct tattgacaag aactataact tttccaggct cagatgcttt
     2281 tttcttcttg tctaagtgat ccttggcaat gtaaacagcc actctggttt ttccactccc
     2341 tgtagggagg cagatgatga tattcttccc ttccaaggct ggctgggcaa cttccatttg
     2401 gtaaggcctg agctggagtt ctggctccgg ggatgctctt gctgccacat tctcttcatc
     2461 tgaatcactt cccatcccgg gtccctgaaa gaggacttca agagccgcgg agtgatggtg
     2521 atggtgatga ccagaaccac tagtcgcttc gtcaccgaac cagcaaatat tacgggcagc
     2581 cataatcagt gctccggctt tttcgtcggt caacccttcg atatcagcca gatcatcaat
     2641 gccctgttcg gcgagatctt ccagcgtaca aacgccacgg gcggccagtt tgaatgccaa
     2701 atcacgatct accccttcaa ggttcagcag atcgtcagcc ggtttgttat caccgaggct
     2761 ttcttcctgg gcctgtgcaa tggtggccag tgcattttta gcacgctcgc gcagtgcttc
     2821 aacggtcggc tcatcaaggc cttcgatttc caacagctct ttcatcggca cataggccaa
     2881 ttcttccagc gtcgagaagc cttcttctac cagaacagtc gcgaagtctt cgtcgatgtc
     2941 gagatatttg gtgaaggtgt cgatcgctgc gtgcgcttcc gcctgatgct tagcttgcag
     3001 gtcgtcaacg gtcatcacgt tgagttccca accgctcagt tgcgaagcca gacgcacgtt
     3061 ctgaccgtta cggccaatcg cctgcgccag attaccggct tcaacggcga tgtccatggt
     3121 gtgtttatct tcatccacca cgatagaagc aacgtctgcc ggtgccattg cgttaatcac
     3181 gaactgcgcc gggttatcat cccacaggac gatatcgata cgctcgccac ccagttcagt
     3241 agacaccgcc tgaacacgcg cgccacgcat acctacgcaa gcacctaccg gatcgatacg
     3301 tttatcgttg gttttcaccg cgattttcgc acgagaaccc ggatcgcgag ccgctgcttt
     3361 aatttcaatc acttcttcgc cgatttctgg cacttcaata cggaacagtt cgatcagcat
     3421 ttccggcttg gaacgagtga cgaacagttg cgcgccacgc gcttccgggc gaacggaata
     3481 gagcacgcca cgaacgcggt cgccagggcg gaagttttca cgcggcagca tatcttcgcg
     3541 caggatcacg gcttcagcgt tgttgcccag atccagagag atgttgtcgc ggtttacttt
     3601 tttcaccacg ccggtgatga tttcaccttc gtgttcacgg aactgatcaa ccaccatcgc
     3661 acgttcggct tcacgcactt tctgcacgat aacctgtttt gccgtctggg tagtgatacg
     3721 gtcaaaggta acagactcaa tctgatcttc aacgtaatcg cccaggttca ggctttcatc
     3781 ttcataacgt gcggcttcaa gggtgatttc cttggtcggc tgggtgactt catcaacaac
     3841 taaccagcga cggaaagtgt caaaatcacc gcttttgcga tcgatctgta cgcggacgtc
     3901 gatctcttgt tcatattttt tctttgttgc tgtcgccagc gcgctttcca atgcttcgaa
     3961 aatcttctcg cgaggtagcg ccttttcatt ggatacggct tcaactacag ccaaaatttc
     4021 tttgttcata ctagaatggt gatggtggtg atggctgctg cccatatgta tatctccttc
     4081 ttaaagttaa acaaaattat ttctagaggg gaattgttat ccgctcacaa ttcccctata
     4141 gtgagtcgta ttaatttcgc gggatcgaga tcgatctcga tcctctacgc cggacgcatc
     4201 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     4261 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     4321 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     4381 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     4441 cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct ttcgcggtat
     4501 ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac cagtaacgtt
     4561 atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg tggtgaacca
     4621 ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg cggagctgaa
     4681 ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc tgattggcgt
     4741 tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga ttaaatctcg
     4801 cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg gcgtcgaagc
     4861 ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga tcattaacta
     4921 tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg ttccggcgtt
     4981 atttcttgat gtctctgacc agacacccat caacagtatt attttctccc atgaagacgg
     5041 tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg cgctgttagc
     5101 gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata aatatctcac
     5161 tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca tgtccggttt
     5221 tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc tggttgccaa
     5281 cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc gcgttggtgc
     5341 ggacatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata tcccgccgtt
     5401 aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc gcttgctgca
     5461 actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac tggtgaaaag
     5521 aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     5581 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     5641 atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg agagccttca
     5701 acccagtcag ctccttccgg tgggcgcggg gcatgactag catgatcgtg ctcctgtcgt
     5761 tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa tcaccgatac
     5821 gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca acaacatgaa
     5881 tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca
     5941 ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca cctacatctg
     6001 tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc cgcatccata
     6061 ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca tcagtaaccc
     6121 gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac agaaatcccc
     6181 cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg gcccgcttta
     6241 tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg gatgaacagg
     6301 cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc
     6361 gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg gtcacagctt
     6421 gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg
     6481 ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat actggcttaa
     6541 ctatgcggca tcagagcaga ttgtactgag agtgcaccat tgcggtgtga aataccgcac
     6601 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     6661 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     6721 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     6781 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     6841 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     6901 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     6961 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     7021 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     7081 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     7141 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     7201 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     7261 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     7321 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     7381 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     7441 cagtggaacg aaaactcacg ttaagggatt ttggtcatga acaataaaac tgtctgctta
     7501 cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt cttgctctag
     7561 gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg ctcgcgataa
     7621 tgtcgggcaa tcaggtgcga caatctatcg attgtatggg aagcccgatg cgccagagtt
     7681 gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga tggtcagact
     7741 aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc gtactcctga
     7801 tgatgcatgg ttactcacca ctgcgatccc cggcaaaaca gcattccagg tattagaaga
     7861 atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc gccggttgca
     7921 ttcgattcct gtttgtaatt gtccttttaa cagtgatcgc gtatttcgtc tcgctcaggc
     7981 gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg agcgtaatgg
     8041 ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct caccggattc
     8101 agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg ggaaattaat
     8161 aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc ttgccatcct
     8221 atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc aaaaatatgg
     8281 tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg agtttttcta
     8341 agaattaatt catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     8401 ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata ttttgttaaa
     8461 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     8521 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     8581 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     8641 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     8701 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     8761 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     8821 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     8881 gggcgcgtcc cattcgccaa tccg
//
